FTIR spectroscopy for estimation of efavirenz in raw material and tablet dosage form

Authors

  • Nareddy Preethi Reddy Department of Pharmaceutical Analysis and Quality Assurance G. Pulla Reddy College of Pharmacy Hyderabad, Telangana
  • Yenumula Padmavathi Department of Pharmaceutical Analysis and Quality Assurance G. Pulla Reddy College of Pharmacy Hyderabad, Telangana
  • Perika Mounika Department of Pharmaceutical Analysis and Quality Assurance G. Pulla Reddy College of Pharmacy Hyderabad, Telangana
  • Akari Anjali Department of Pharmaceutical Analysis and Quality Assurance G. Pulla Reddy College of Pharmacy Hyderabad, Telangana

DOI:

https://doi.org/10.3329/icpj.v4i6.23290

Keywords:

Efavirenz, FTIR, method validation, ICH guidelines

Abstract

A Fourier transform infrared (FTIR) spectrophotometric method was developed for rapid and direct measurement of efavirenz in pharmaceutical formulations. The method involves extraction of efavirenz from tablets with chloroform by sonication and the direct measurement of the absorbance in liquid phase using a reduced path length cell. In general, the spectrum was measured in transmission mode. The equipment was configured to collect a spectrum at 8 cm-1 resolution and 45 scans per sec .The spectra were collected between 4000 cm-1 and 450cm-1, the band obtained at 1750cm-1 (carbonyl group) showed intense, clear peak in the liquid phase for quantitation. The method was validated as per ICH guidelines. The method fulfilled most validation requirements in the linearity range 200-1000µg/mL. The coefficient of determination, limit of detection and quantification was found to be 0.993, 49.12?g/mL and 148.84?g/mL respectively. Results of developed FTIR method were compared with the results obtained with the existing UV method statistically by using t-test, which indicated that there is no significant difference between the methods at P=0.05. The proposed FTIR method reduces the solvent consumption and also eliminates the use of reagents. Thus the developed method offers a good alternative for the quantitative estimation of efavirenz in bulk and pharmaceutical dosage forms and also to quantify efavirenz when combined with other API in the same dosage form.

International Current Pharmaceutical Journal, May 2015, 4(6): 390-395

Downloads

Download data is not yet available.
Abstract
1211
PDF
2252

Downloads

Published

2015-05-11

How to Cite

Reddy, N. P., Padmavathi, Y., Mounika, P., & Anjali, A. (2015). FTIR spectroscopy for estimation of efavirenz in raw material and tablet dosage form. International Current Pharmaceutical Journal, 4(6), 390–395. https://doi.org/10.3329/icpj.v4i6.23290

Issue

Vol. 4 No. 6 (2015)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).