Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

Authors

  • B. Janarthanam Omnigreen Organic Biopark Pvt. Ltd., Plant Biotechnology Division, lwarthirunagar, Chennai- 600 087, Tamil Nadu
  • P. Rashmi Department of Microbiology, Valliammal College for Women, E-9, Anna Nagar East, Chennai - 600 102, Tamil Nadu
  • E. Sumathi Department of Biotechnology, D.G. Vaishnav College, University of Madras, Arumbakkam, Chennai-600106, Tamil Nadu

DOI:

https://doi.org/10.3329/ptcb.v22i1.11257

Keywords:

Artemisia annua, plant regeneration, nodal explant, mass propagation

Abstract

An efficient plant regeneration protocol was developed for Artemisia annua, an aromatic medicinal herb. Nodal explants inoculated on MS supplemented with 4.44 ?M BAP showed better growth response and produced 116.2 ± 0.1 micro-shoots of an average length 1.9 ± 0.3 cm after 35 days culture. The cluster of small shootlets were cultured on shoot elongation medium supplemented with 1.44 ?M GA3 and 10% coconut milk (CM) showed shoot elongation up to 4.6 ± 0.7 cm. Roots were induced after transfer to half strength MS supplemented with 2.46 ?M IBA produced 14.3 ± 0.2 roots with an average height of 4.3 ± 0.53 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 :  1 : 1).

DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11257

Plant Tissue Cult. & Biotech. 22(1): 33-39, 2012 (June)

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Published

2012-07-01

How to Cite

Janarthanam, B., Rashmi, P., & Sumathi, E. (2012). Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L. Plant Tissue Culture and Biotechnology, 22(1), 33–39. https://doi.org/10.3329/ptcb.v22i1.11257

Issue

Vol. 22 No. 1 (2012)

Section

Articles