biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 65:212-220, 2021 | DOI: 10.32615/bp.2021.012

Transcriptome comparison of physiological divergence between two ecotypes of Portulaca oleracea

M. WU1, 2, S. FU1, 2, 3, W. JIN1, 2, W.Z. XIANG1, 2, W.C. ZHANG1, 2, L. CHEN1, 2, 3, *
1 Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, Huangshi 435002, P.R. China
2 The College of Life Science, Hubei Normal University, Huangshi 435002, P.R. China
3 Hubei Engineering Research Center of Special Wild Vegetables Breeding and Comprehensive Utilization Technology, Huangshi 435002, P.R.China

According to the survey, wild purslane (Portulaca oleracea L.) has two different ecotypes, one with the red stem living in an arid environment and one with the green stem living in a humid environment. In order to explore the physiological response strategies of plants to environmental changes, these two ecotypes of purslane were selected as experimental materials. Physiological indices were determined and transcriptome analysis was carried out to screen the differentially expressed genes (DEGs) from two ecotypes of purslane. The results showed that the content of soluble sugars, proline, anthocyanins, and chlorophylls was significantly different, and most DEGs belonged to the WRKY and NAC families. Finally, the results of transcriptome analysis were verified by real-time qPCR. Therefore, it can be inferred that the transcription factor (TF) families may play an important role in physiological response strategies by regulating the changes of anthocyanins and osmotic regulators (soluble sugars, proline, etc.) through the abscisic acid signalling pathway. This will accelerate the study of purslane at the molecular level, provide corresponding theoretical support for its artificial domestication, and drought breeding.

Keywords: anthocyanin, chlorophyll, differential gene expression, Portulaca oleracea, proline, purslane ecotypes, stress tolerance, sugars.

Received: November 19, 2020; Revised: February 15, 2021; Accepted: February 19, 2021; Published online: July 29, 2021  Show citation

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WU, M., FU, S., JIN, W., XIANG, W.Z., ZHANG, W.C., & CHEN, L. (2021). Transcriptome comparison of physiological divergence between two ecotypes of Portulaca oleracea. Biologia plantarum, 65, Article 212-220. https://doi.org/10.32615/bp.2021.012
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