Photosynthetica 2020, 58(5):1116-1121 | DOI: 10.32615/ps.2020.060

Upregulation of mitochondrial alternative oxidase pathway protects photosynthetic apparatus against photodamage under chilling stress in Rumex K-1 leaves

D. CHENG1,2, H. GAO2, L. ZHANG3
1 College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong, China
2 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taiꞌan, Shandong, China
3 Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, China

This study aimed to clarify the role of mitochondrial alternative oxidase (AOX) pathway upregulation in photoprotection under chilling stress in Rumex K-1 leaves. Malate-oxaloacetate shuttleꞌs activity and AOX pathwayꞌs capacity were synchronously enhanced with light intensity increase both in control and chilling-treated leaves. Besides, the AOX pathway was upregulated more significantly when irradiated by light in chilling-treated leaves than that of control. These results suggested that excess reducing equivalents generated by photosynthesis were exported from chloroplasts and oxidized by the AOX pathway at a potentially higher rate in chilling-treated leaves than in control, which avoided the overaccumulation of excess reducing equivalents under light conditions. Also, inhibition of the AOX pathway led to more severe accumulation of hydrogen peroxide in chilling-treated leaves than that in control. Therefore, the mitochondrial AOX pathway was specifically upregulated and employed for sinking excess reducing equivalents produced by photosynthesis, which protected photosynthetic apparatus from photodamage under chilling stress in Rumex K-1 leaves.

Additional key words: abiotic stress; photosynthesis; respiratory metabolism.

Received: May 6, 2020; Revised: August 6, 2020; Accepted: August 12, 2020; Prepublished online: September 24, 2020; Published: December 8, 2020  Show citation

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CHENG, D., GAO, H., & ZHANG, L. (2020). Upregulation of mitochondrial alternative oxidase pathway protects photosynthetic apparatus against photodamage under chilling stress in Rumex K-1 leaves. Photosynthetica, 58(5), 1116-1121. doi: 10.32615/ps.2020.060
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