Photosynthetica 2020, 58(SI):460-467 | DOI: 10.32615/ps.2019.178

Special issue in honour of Prof. Reto J. Strasser – Chlorophyll fluorescence of Nicotiana tabacum expressing the green fluorescent protein

N. KEUTGEN1, M. TOMASZEWSKA-SOWA2, A.J. KEUTGEN1
1 BOKU - University of Natural Resources and Life Sciences, Institute of Vegetables and Ornamentals at the Department of Crop Sciences, Gregor Mendel Str. 33, 1180 Vienna, Austria
2 UTP University of Science and Technology, Department of Agricultural Biotechnology, Laboratory of Genetics and Plant Physiology, Bernardyńska 6, 85-029 Bydgoszcz, Poland

Evidence that the green fluorescence protein (GFP) develops a significant toxicity in plants has not been found, but it may represent a source of free radicals as a consequence of its fluorescence. In addition, green light is known to trigger the acclimatisation of the photosynthetic system towards a shady environment. Moreover, the light-harvesting system may acclimate to an increased availability of green light. Each of these effects may be induced by the GFP. Therefore, the hypothesis was tested, whether transformation of Nicotiana tabacum cv. Bursan to express the GFP could affect chlorophyll fluorescence parameters. The analysis revealed a significantly lower absorption of energy per excited cross section in GFP-transformed tobacco, a lower number of active reaction centres per excited cross section, a larger absorption and trapped energy flux leading to the reduction of the primary quinone electron acceptor of PSII per reaction centre, and a lower variable fluorescence.

Additional key words: low-light stress; OJIP transient; steady-state procedure; toxicity of GFP.

Received: July 14, 2019; Revised: December 9, 2019; Accepted: December 18, 2019; Prepublished online: February 13, 2020; Published: May 28, 2020  Show citation

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KEUTGEN, N., TOMASZEWSKA-SOWA, M., & KEUTGEN, A.J. (2020). Special issue in honour of Prof. Reto J. Strasser – Chlorophyll fluorescence of Nicotiana tabacum expressing the green fluorescent protein. Photosynthetica, 58(SPECIAL ISSUE), 460-467. doi: 10.32615/ps.2019.178
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