biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 64:454-464, 2020 | DOI: 10.32615/bp.2019.069

The rice Aux/IAA transcription factor gene OsIAA18 enhances salt and osmotic tolerance in Arabidopsis

G. LI1, Y.X. YE2, X.Q. REN2, M.Y. QI2, H.Y. ZHAO2, Q. ZHOU2, X.H. CHEN2, J. WANG2, C.Y. YUAN1,*, F.B. WANG2,*
1 Huaiyin Institute of Agricultural Sciences of Xuhuai Region, Huai'an Jiangsu 223001, P.R. China
2 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an Jiangsu 223003, P.R. China

In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators, which regulate developmental process and responses to phytohormones and stress treatments. A previous study has shown that the rice Aux/IAA transcription factor gene OsIAA18 is induced by salt and osmotic stresses. However, little is known about the regulatory functions of this gene. In this study, the OsIAA18 gene was successfully cloned from rice. Subcellular localization analysis in onion epidermal cells indicated that OsIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length OsIAA18 exhibited transcriptional activation. Heterologous expression of OsIAA18 significantly enhanced salt and osmotic tolerance in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of OsIAA18 up-regulated genes involved in abscisic acid (ABA) biosynthesis, proline biosynthesis, stress responses, and reactive oxygen species scavenging under salt and osmotic stresses. Enzymatic analyses found that the transgenic plants had higher 9-cis-epoxycarotenoid dioxygenase, pyrroline-5-carboxylate synthase, superoxide dismutase, and peroxidase activities than wild-type plants under salt and osmotic stresses. In the transgenic plants, ABA and proline content significantly increased, whereas H2O2 and malondialdehyde content significantly decreased. In addition, the transgenic plants had also a lower electrolyte leakage and water loss rate. These overall results indicate that the OsIAA18 gene is involved in enhancing salt and osmotic tolerance in transgenic Arabidopsis plants. The OsIAA18 gene has a potential to be used to enhance the tolerance to abiotic stresses in other plant species.

Keywords: abscisic acid, onion, Oryza sativa, MDA, POD, proline, ROS, SOD, yeast.

Received: December 10, 2018; Revised: April 15, 2019; Accepted: April 23, 2019; Published online: June 25, 2020  Show citation

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LI, G., YE, Y.X., REN, X.Q., QI, M.Y., ZHAO, H.Y., ZHOU, Q., ... WANG, F.B. (2020). The rice Aux/IAA transcription factor gene OsIAA18 enhances salt and osmotic tolerance in Arabidopsis. Biologia plantarum, 64, Article 454-464. https://doi.org/10.32615/bp.2019.069
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