Removal of Melachite Green Dye by Using Zinc Oxide Nanoparticles Prepared by The Green Synthesis by Using Camellia Sinensis (Green Tea) Leafs Extract

Green synthesis for nanoparticles using microorganisms, enzymes, and plants leaf or plant extracts have been indicated as possible ecofriendly preferences to chemical and physical methods. In this paper, we report the synthesis of zinc oxide particles by green method. Highly stable and hexagonal zinc oxide nanoparticles were produced by using zinc nitrate and green tea leaf extract. Structural, morphological and optical properties of the synthesized nanoparticles have been characterized by using UV–Vis spectrophotometer, FTIR, SEM, and XRD analysis. The synthesis of nanoparticles of zinc oxide was observed by the color changing of the chemical solution. Powder X-ray diffraction and SEM analysis revealed the synthesis of both Zn and ZnO nanoparticles with average particle size of 60nm. Shape of zinc oxide nanoparticles was hexagonal with the range 80-120nm. UV spectra confirmed the presence of zinc oxide nanoparticles. FTIR showed the peaks of zinc oxide nanoparticles as well as the presence of other compounds. Melachite green dye has been removed by using zinc oxide nanoparticles prepared from green tea leaf extract. The green color of the dye was removed up to 70% with the use of reducing agent sodium borohydrate. Removal of melachite green dye was performed. Other applications of zinc oxide nanoparticles in the medicine, pharma, biotechnology and industries were discussed.


Introduction
Nanotechnology literally means any technology on a nanoscale. Nanotechnology encloses the yeild and application of physical, chemical, and biological systems at scales ranging from individual atoms or molecules to submicron dimensions, as well as the involvement of the nanostructures that are formed into the larger systems. Nanotechnology continue to move forward in making the fabrication of micro/nanodevices and systems possible for a variety of industrial, consumer, and biomedical applications also used in various fields like drugs and medicines, electronics, food, fuel cells, solar cells, batteries, in space, chemical censors and fabric etc [1]. The biosynthesis of nanoparticles has been proposed as a cost-effective and environmentally friendly alternative to chemical and physical methods. Plant-mediated synthesis of nanoparticles is a green chemistry approach that connects nanotechnology with plants. Green synthesis has been involved in synthesis of highly stabilized nanoparticles. Nanoparticles integrity maintenance is the challenge by green synthesis. Zinc oxide nanoparticles have been synthesized by leaf extract of green tea. It is a common name of Chinese white [2]. The green synthesis of zinc oxide nanoparticle is fast, economically feasible, and sufficient method. Phenolic content in plant extract which dissolves in water, biodegradable and catalyze synthesis of nanoparticle as capping and reducing agent [3].
Green tea was used as a medicine India as well as in Chinese medicine. Green tea ensures better health and longevity. Recent studies have also shown green tea can potentially have positive effects on everything from weight loss to liver disorders, type 2 diabetes, and Alzheimer disease. Unsweetened green tea is totally

Removal of Melachite Green Dye by Using Zinc Oxide Nanoparticles Prepared by The Green Synthesis by Using Camellia Sinensis (Green Tea) Leafs Extract
contains 0% calorie in it. The amount of caffeine in a cup of tea can changes according to the length of insinuate time and the amount of tea. Basically, green tea contains a moderately small amount of caffeine. Almost 20-45mg per 8-ounce cup is present in it. Green tea is one of the world's healthiest drinks that contains one of the greatest amounts of antioxidants of any tea. Natural chemicals such as polyphenols in tea provides its anti-inflammatory and anticarcinogenic effects. Green tea has almost 20-45% polyphenols by weight, in which 60-80% are catechins such as EGCG. Catechins are antioxidants and helps to prevent cell damage [4] (Figures 1 & 2).   Figure 3: Green tea leaf extract.

Material
Green tea leaf was collected from garden, zinc nitrate salt, distilled water, sodium borohydrate, oxalic acid, methanol.

GREEN Synthesis of ZnO NPs
zinc nitrate solution of fifty milliliter was added to 5ml of green tea leaf extract. The solution was stirred on magnetic stirrer at 120 degrees [6]. The color change was observed from light brown to blackish brown. The resultant solution was centrifuged for ten minutes at speed of 50,000rpm. After discarding suspended zinc oxide nanoparticles were dried in watch glass. Yellow colored particles were collected for characterization [7]

Color Change Observation
When the mixture of salt zinc nitrate and green tea leaf extract was added and heat the color changes appeared. Color changes Arch Nano Op Acc J

X-Rays Diffraction Studies
This technique basically tells us about the crystal density, purity and size of the metal nanoparticle prepared. Zinc oxide nanoparticles were examined by X-ray diffractometer (XPERT-PRO). Zinc oxide powder was put in cubes of XRD for calculation of intensity. The resultant pattern of synthesized zinc oxide nanoparticles was analyzed [10].

Scanning Electron Microscope (Sem)
This technique tells us about the particle size of metallic

FTIR Analysis
In this study FTIR spectrum was analyzed to confirm zinc oxide nanoparticles. Peaks were observed at 457cm -1 and 545cm -1 shows that zinc oxide nanoparticles are present. Other peaks indicate presence of other groups. OH, stretch of hydrogen group gives peak at 3215.08cm -1 . Methoxy methyl ether CH stretch gives peak at 2964.17cm -1 . Alkenyl C=C stretch is present at 1636.68cm -1 . Amine NH bend gives peak at 1541.82cm -1 . Aromatic phosphate P-O-C stretch at 1240.07cm -1 is present in Figure 9.

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of zinc oxide nanoparticles. The main advantage of this procedure is that it is easily available, non-costly and is easy to handle. As the sample used is non-toxic thus is pollution free. The nanoparticles prepared have been used in various industries of medicine, pharma, biochemistry and fabric etc [10] (Figure 10) and (Table   1).

Dye Removal by Zno Nanoparticles
1mg of zinc oxide nanoparticles were taken then using 20mg of dye solution we add those ZnO nanoparticles. 0.1mg of sodium borohydrate was added as a reducing agent. The color began to fade. Firstly, the peak of melachite green was noted in UV that was 621nm. On color changes the peaks were noted in the UV spectrophotometer with specific time intervals. The wavelength begins to decrease from 621nm. After the color has almost fade out the peak was noted that was 0nm ( Figure 11).

Melachite Green Dye
Malachite green having chemical formula C 2 3H 2 5CIN 2 (chloride) or [C 6 H 5 C(C 6 H 4 N(CH 3 ) 2 ) 2 ] Cl is normally used as a dye. It has a molar mass of 364.911g/mol. The intense green color of the dye results from a strong absorption band at 621nm in UV visible spectra. This dye is moderately toxic and extremely irritant [12].

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remove. This color removal confirms the rapid reaction of zinc ( Figure 13).

P H Effect on Dye Removal:
De-colorization of dye is strongly affected by P H of the solution. In this research we have studied the PH effect on decolorization of dye using zinc oxide nanoparticles.
Zinc oxide nanoparticles showed a maximum de-colorization on increasing the P H but after a certain limit the more increase has a negative impact. This may happen due to the formation of more positive ions. Maximum de-colorization at 70⁒ have the P H value of 3.

Concentration of Dye
The concentration of dye plays an important role in the decolorization process. A graph was drawn after the experiment on different concentrations of dye. The maximum amount taken was 10mg/l. after increasing the concentration of dye effect on 70⁒ decolorization of dye was observed.

Effect of Concentration of Zinc Oxide Nanoparticles
The amount of zinc oxide nanoparticles has a positive response to the de-colorization of dye. Firstly 1mg/l was taken and after some time interval the color was removed which prove that further increase in zinc oxide nanoparticles concentration will not affect the de-colorization of dye? (Figure 14).

Conclusion
In conclusion here we report an eco-friendly, feasible and short time preparation of zinc oxide nanoparticles by leaf extract of green tea. This method has an advantage over other reported methods as it is easily available starting materials, not costly and the procedure is easy to handle in the laboratory, no toxic reagent is used and pollution free. UV spectra of prepared particles was tested in UV spectrophotometer which was 320nm. it shows the wavelength range of nanoparticles. Highest peak shows the maximum absorbance. FTIR spectra confirmed the presence of zinc oxide nanoparticles. Also, other peaks were obtained showing presence of other groups and their stretching and bending was also defined. XRD and SEM defined the shape and size of nanoparticles which was hexagonal and the size range was about 80-120nm. XRD confirmed the presence of both zinc and zinc oxide nanoparticles.
The mineral name is zincite. Its common name is Chinese white.
The space group is 186.the calculated density was 5.68g/cm 3 . and measured density was 5.66g/cm 3 . <0.001⁒ of each Mg, Si, Ca were present. The temperature at which we collected data was 26℃.
An application of dye removal is of great importance which was performed by using malachite green dye. The green color of dye was removed using zinc oxide nanoparticles that were prepared by using green tea leaf extract. The effects of temperature, P H , concentration of both the dye and nanoparticles on de-colorization was also studied. The maximum de-colorization obtained was 70⁒.
At the end the color was removed. When the color was removed by nanoparticles the last P H noted was 3 by using P H paper.
Nanoparticles of zinc oxide synthesized can also be applicable in different field of biochemistry, medicine and industry.