Dengue Outbreak Response during COVID-19 Pandemic, Key Largo, Florida, USA, 2020

We report a dengue outbreak in Key Largo, Florida, USA, from February through August 2020, during the COVID-19 pandemic. Successful community engagement resulted in 61% of case-patients self-reporting. We also describe COVID-19 pandemic effects on the dengue outbreak investigation and the need to increase clinician awareness of dengue testing recommendations.

provider and community outreach, collected serum samples for DENV testing, and promptly provided updates to FKMCD and local media.Persons with suspected dengue were asked to provide contact information for other persons who shared mosquito exposure risks, such as persons from the same household, workplace, or outdoor events.FDOH reached out to contacts and offered DENV testing if they reported a recent unexplained febrile illness.Ethics approval was not required because the activities conducted were part of standard public health outbreak surveillance and response.
FDOH also conducted syndromic surveillance for chief complaint and discharge diagnosis records from local hospitals.FDOH reviewed all syndromic surveillance records in the primary hospital serving the outbreak area and countywide, prioritizing chief complaints and discharge diagnoses mentioning dengue or fever and any combination of thrombocytopenia, rash, or arthralgia.FDOH requested medical records for patient visits with no alternative diagnosis.If only dengue serology had been ordered, FDOH requested that specimens be forwarded to the state laboratory for reverse transcription PCR (RT-PCR) testing.If no alternative diagnosis had been made and no DENV testing previously ordered, FDOH offered testing for persons with suspected cases.
Consistent with Centers for Disease Control and Prevention (CDC) guidelines, FDOH tested acute specimens collected within 7 days after symptom onset by using DENV RT-PCR and IgM tests.We only routinely performed antibody testing on convalescent samples collected >7 days after symptom onset.Specimens with positive or equivocal DENV test results at commercial laboratories were forwarded to FDOH and similarly retested.CDC assisted with serologic confirmation, serotyping RT-PCR-positive samples, and provided RT-PCR testing for mosquito pools collected by FKMCD.We identified 72 locally acquired dengue cases associated with Key Largo.Cases were primarily among female (51%) and non-Hispanic (83%) persons (Table ).Self-reporting, including via contact outreach, drove initial case identification (61%), followed by commercial laboratory reporting (22%), and syndromic surveillance (7%); only 1 case was first identified through direct healthcare provider reporting.No case-patients had traveled outside the continental United States during the incubation period.
Overall, 31 cases were RT-PCR-positive and 41 were IgM-positive (Table ).All RT-PCR-positive cases were DENV-1.Retrospective case finding and testing identified IgM-positive cases with reported symptom onset as early as January (Figure 1).Some persons identified through retrospective case finding reported a febrile illness several months prior.We presumed those febrile illnesses were dengue, but asymptomatic infections are common, and IgM is generally detectable for 3 months, making definitive confirmation of the timing of DENV infection difficult.
Among 96 Ae. aegypti mosquito pools collected during June 18-September 21, three tested positive for DENV-1 (Figure 1).We sequenced 15 positive samples, 12 from dengue cases and 3 from mosquito pools.Phylogenetic analysis showed grouping within the Caribbean lineage of DENV-1 genotype V (Figure 2).Sequences from mosquito pools and humans were almost identical.We published sequence data in Gen-Bank (accession nos.OM831209-18, OM833055-59, and OM909246-47).Sequencing definitively differentiated this outbreak of Caribbean lineage DENV from the 2009-2010 Key West outbreak of Central American lineage.
Among case-patients, 43 (60%) visited a healthcare provider during the acute illness, within 1 week after symptom onset (Table ).Providers considered COVID-19 as a potential diagnosis, which is evidenced by COVID-19 test orders for 38 (88%) of the acute dengue cases.Dengue was considered a potential diagnosis in only 16 acute cases, 13 of which had testing for both dengue and COVID-19.Providers primarily (75%) ordered dengue antibody testing when evaluating acute cases, which is inconsistent with CDC recommendations to use RT-PCR or DENV nonstructural protein 1 (NS1) test (10), an alternative to RT-PCR, in addition to IgM testing during the acute phase.No acute samples were tested using the DENV NS1 test.Among acute samples, 26 had comprehensive testing (both RT-PCR and IgM) performed at a reference laboratory at FDOH or CDC.Eight were positive for both assays, 14 were only DENV RT-PCR-positive, and 4 were only DENV IgM-positive.Ultimately, 54% of acute cases tested with only an IgM assay would have been missed if not for additional RT-PCR testing performed at a reference laboratory, compared with just 15% missed by using RT-PCR testing alone.

Conclusions
This investigation confirmed an ongoing dengue outbreak in the Key Largo area of Florida, USA, during January-August 2020.During that same timeframe, 1,692 COVID-19 cases were reported in Monroe County.We suspect the COVID-19 pandemic negatively affected dengue surveillance because of reluctance to seek medical care, competing demands on healthcare providers during a rapidly evolving pandemic, and similar clinical presentations between COVID-19 and dengue.The focus on COVID-19 was further evidenced by providers primarily ordering COVID-19 tests among patients with acute dengue seeking medical care.The use of multiple case-finding methods, including aggressive community engagement, helped mitigate some of those effects, as did pandemic-related travel restrictions in the county.
In conclusion, CDC recommends using either commercial DENV RT-PCR or NS1 tests in combination with serologic testing for samples collected during the acute phase of dengue illness (10).This outbreak highlights that those tests were underused.Improving clinician awareness of CDC recommendations could improve case detection in the future, especially for nonendemic areas at increased risk for DENV introduction.
Emerging Infectious Diseases • www.cdc.gov/eid• Vol. 29, No. 8, August 2023 Table.Characteristics of case-patients in a dengue outbreak during the COVID-19 pandemic, Key Largo, Florida, USA, 2020* *DENV, dengue virus; RT-PCR, reverse transcription PCR.†Samples for PCR collected 1-12 days after symptom onset; samples for IgM collected 5-213 days after symptom onset.‡Acute cases were tested within 7 days after symptom onset.Data include only persons medically evaluated by a healthcare provider in the United States.Information on care-seeking behavior was determined through case interview and laboratory records.Negative COVID-19 results were required to be reported to Florida Department of Health, but negative dengue laboratory results are not, which might have resulted in underreporting of commercial dengue testing among persons who sought care.