mcr-1 Colistin Resistance in ESBL-Producing Klebsiella pneumoniae, France

We report intestinal carriage of an extended-spectrum β-lactamase−producing Klebsiella pneumoniae strain with high-level resistance to colistin (MIC 24 mg/L) in a patient in France who had been hospitalized for fungal meningitis. The strain had the mcr-1 plasmid gene and an inactivated mgrB gene, which are associated with colistin resistance.


mcr-1 Colistin Resistance in ESBL-Producing
We report intestinal carriage of an extended-spectrum β-lactamase−producing Klebsiella pneumoniae strain with high-level resistance to colistin (MIC 24 mg/L) in a patient in France who had been hospitalized for fungal meningitis. The strain had the mcr-1 plasmid gene and an inactivated mgrB gene, which are associated with colistin resistance. R esistance to colistin in gram-negative bacteria stems mainly from structural modifications of bacterial lipopolysaccharide. These modifications include addition of 4-amino-4-deoxy-l-arabinose or phosphoethanolamine caused by chromosomal mutations in genes encoding the 2-component systems PhoPQ and PmrAB, or mutations in the mgrB gene, a negative regulator of PhoPQ (1).
The recent discovery of a horizontally transferable plasmid-mediated mcr-1 gene encoding a phosphoethanolamine transferase is a cause for concern, but few mcr-1−positive clinical strains of Klebsiella pneumoniae have been reported so far in Europe (2). Colocalization of carbapenemases or extended-spectrum β-lactamase (ESBL) genes and the mcr-1 gene on the same plasmids is of concern because it might lead to pandrug resistance (1,3). We report mcr-1 colistin resistance in ESBL-producing K. pneumoniae isolated from a patient in France.
The patient was a 38-year-old man who had chronic granulomatous disease that was diagnosed when he was 8 months old. Since then, he has had several minor and major diseases and conditions, including primitive femoral osteitis, hepatic abscesses, disseminated candidiasis, and bacteremia, which required several treatments with antimicrobial drugs. However, the patient was never given colistin.
In April 2016, he was hospitalized for surgical removal of a thyroid abscess. Fungal cultures of the abscess grew Aspergillus fumigatus. Despite antifungal treatment with amphotericin B and flucytosine, fungal meningitis, cerebral arterial vasospasm at the Willis polygon, and hydrocephalus developed. The patient also received immunosuppressive therapy (methylprednisolone and anakinra) and empiric antimicrobial drug therapy, including cotrimoxazole, clindamycin, meropenem, and vancomycin successively.
The strain was sent to the French National Reference Center for Antibiotic Resistance in Enterobacteriaceae (Hôpital Gabriel Montpied, Clermont-Ferrand, France), which confirmed phenotypic resistance to colistin and identified the mcr-1 gene by using PCR and previously described primers (2). Whole-genome sequencing showed that the K. pneumoniae strain had genotype ST15 and confirmed the presence of the mcr-1 gene on a 33,303-kb transferable plasmid of incompatibility group IncX4 (online Technical Appendix). This plasmid differed by only 4 mutations from mcr-1.2−encoding plasmid pMCR-1.2.IT (GenBank accession no. KX236309) previously characterized in Italy (4). Conjugation of the plasmid into Escherichia coli K12 conferred colistin resistance (MIC increased from 0.25 mg/L to 4 mg/L) to the E. coli strain.
Other resistance genes were also identified (Table), including the ESBL-encoding gene bla SHV106 (online Technical Appendix). None of them were localized with the mcr-1 gene on the IncX4 plasmid. Moreover, insertion of mobile element IS5 in the mgrB gene was detected, which is also associated with colistin resistance (5). No mutations were found in the prmA, prmB, phoP, and phoQ genes.
There is currently no commercial medium to screen gram-negative bacteria harboring the mcr-1 gene. Nordmann et al. (6) described an in-house SuperPolymyxin medium composed of eosin methylene blue agar, 3.5 mg/L of colistin sulfate, 10 mg/L of daptomycin, and 5 mg/L amphotericin B, which showed excellent sensitivity and specificity. Colistin resistance can be confirmed within 2 h by using an in-house rapid polymyxin Nordmann-Poirel test (7). The mcr-1 gene can be rapidly detected by real-time PCR of DNA extracts obtained from bacterial strains or directly from stool samples (2,8,9).
We obtained subcultures of the strain from the patient on Columbia CNA agar containing 10 mg/L of colistin and 15 mg/L of nalidixic acid and 5% sheep blood (CNA + ; bio-Mérieux) but not on Thayer-Martin agar medium containing unknown concentrations of vancomycin, colistin, amphotericin B, and trimethoprim (VCA3; bioMérieux). Lack of growth on this medium might be related to a high colistin concentration or the presence of vancomycin, which can potentiate colistin activity (6). Further investigations using  The origin of the mcr-1 strain remains unknown. Nosocomial acquisition cannot be ruled out because colistinresistant strains harboring the mcr-1 gene might have been isolated in the hospital but not identified because this resistance mechanism was initially reported in February 2016. Food might also be incriminated (1); one study identified a 21% mcr-1 prevalence among ESBL-producing E. coli in calves in France (10).
Multiple antimicrobial drug therapy for this patient might have selected for this multidrug-resistant bacteria. The presence of a plasmid containing the mcr-1 and ESBL or other resistance genes in the same strain might be involved in selection of colistin-resistant strains during administration of any ineffective antimicrobial drug (3). Development of efficient tools for rapid detection of mcr-1−harboring strains should be a priority to prevent dissemination of these strains in hospital settings.
Dr. Caspar is a clinical microbiologist in the Bacteriology Laboratory, Grenoble-Alpes University Hospital, and a probationary lecturer in the Medicine Faculty, Grenoble-Alpes University, Grenoble, France. His primary research interests are bacterial resistance or treatment failures for tularemia and evaluation of novel antimicrobial compounds.
We identified rmtE1, an uncommon 16S ribosomal methyltransferase gene, in an aminoglycoside-and cephalosporin-resistant Escherichia coli sequence type 448 clinical strain co-harboring bla CMY-2 . Long-read sequencing revealed insertion of a 101,257-bp fragment carrying both resistance genes to the chromosome. Our findings underscore E. coli sequence type 448 as a potential high-risk multidrug-resistant clone.
R mtE (RmtE1 and its variant RmtE2) is an uncommon plasmid-mediated 16S rRNA methyltransferase (16S RMTase) found in gram-negative bacteria; only 4 strains have been reported to produce RmtE, all Escherichia coli, including 1 from the University of Pittsburgh Medical