Rhodococcus Infection in Solid Organ and Hematopoietic Stem Cell Transplant Recipients

We conducted a case–control study of 18 US transplant recipients with Rhodococcus infection and 36 matched controls. The predominant types of infection were pneumonia and bacteremia. Diabetes mellitus and recent opportunistic infection were independently associated with disease. Outcomes were generally favorable except for 1 relapse and 1 death.

We conducted a case-control study of 18 US transplant recipients with Rhodococcus infection and 36 matched controls. The predominant types of infection were pneumonia and bacteremia. Diabetes mellitus and recent opportunistic infection were independently associated with disease. Outcomes were generally favorable except for 1 relapse and 1 death. R hodococcus, a gram-positive coccobacillus, has been isolated from water, soil, and the manure of herbivores. It is a facultative intracellular pathogen that survives in host macrophages. Immunosuppressive medications that compromise cell-mediated immunity can predispose to infection (1,2).
Our knowledge of disease characteristics among transplant recipients is limited to case reports (3)(4)(5)(6)(7)(8). With the increase in organ transplantation and improved survival of transplant recipients, the incidence of disease will likely increase in the coming years. In this study, we sought to describe characteristics, risk factors, and outcomes of Rhodococcus infection among solid organ transplant (SOT) and hematopoietic stem cell transplant (HSCT) recipients in the United States.

The Study
We conducted a case-control study at 8 US medical centers during January 2000-December 2012. The study was approved by appropriate Institutional Review Boards. Casepatients were those with clinical or radiographic features of infection and positive culture results for Rhodococcus spp. Identification of the organism was performed by using biochemical methods at microbiology laboratories of participating institutions. At the discretion of laboratory staff, identification was confirmed by using 16S rRNA sequencing.
Controls received a similar organ within 3 months before or after the index case-patient at the same center and did not show Rhodococcus infection. Each case-patient was matched with 2 controls. Allogeneic and autologous HSCT recipients were matched to recipients of the same type.
We used descriptive statistics to summarize results for the cohort. Conditional logistic regression was used to evaluate risk factors for infection. Factors associated with disease less than the 0.10 significance level for univariate analysis were included in the multivariate model. Statistical software Stata/SE version 13.1 (StataCorp LP, College Station, TX, USA) was used.
Median time to diagnosis after onset of symptoms was 20 (range 2-67) days. This median was determined mainly by the time that the patient sought medical attention and the time of clinical specimen collection. At the time of diagnosis, 3 (17%) patients were managed in outpatient settings, 12 (67%) in inpatient wards, and 3 (17%) in intensive care units.
Infected patients were matched with 36 controls. Univariate analysis showed that type of immunosuppression, augmented immunosuppression, increased levels of tacrolimus or cyclosporine, and trimethoprim/sulfamethoxazole (TMP/SMX) prophylaxis were not associated with infection. Multivariate analysis showed that diabetes mellitus (p = 0.041) and recent opportunistic infection (p = 0.045) were independently associated with infection ( Table 2).
Most patients received combination treatment with 2-3 antimicrobial drugs (Table 1). Most commonly used drugs in the initial regimen were vancomycin, a fluoroquinolone, or a carbapenem. Median duration of treatment was 1 month (range 2 weeks-7 months) for patients with cathether-associated bacteremia and 6 months (range 2-60 months) for patients with all other infections. Immunosuppression was decreased in 44% (7/16). The patient with a pacemaker pocket infection had the device removed.
Median follow-up period was 17 (range 1-84) months. One allogeneic HSCT recipient with R. equi pneumonia died of respiratory failure 13 days after diagnosis. He was receiving effective treatment with levofloxacin and TMP/ SMX. One allogeneic HSCT recipient with bacteremic cavitary pneumonia who received 6 months of antimicrobial drug treatment had disease relapse (fever, cough, and dyspnea) 9 months after initial presentation. Rhodococcus spp. were recovered from bronchoalveolar lavage fluid at relapse. The 4 patients with catheter-associated bacteremia had their catheters removed and did not show relapse.

Conclusions
Our study showed an association between Rhodococcus infection and preceding opportunistic infection. This finding suggests that affected patients have a high net state of immunosuppression. Prior cytomegalovirus infection, the most common opportunistic infection in the study, might have had an immunomodulatory effect that made patients more likely to show development of a second opportunistic infection.
Most patients were not neutropenic at diagnosis, consistent with the fact that Rhodococcus spp. affect mainly patients with impaired cell-mediated immunity. TMP/ SMX prophylaxis did not confer protection, probably because of high resistance rates. Patients did not always have a history of exposure to livestock as previously described (2). Median time to infection was shorter for HSCT recipients, probably because catheter-associated bacteremia  (2), pulmonary aspergillosis (1), and BK polyomavirusassociated hemorrhagic cystitis (1). was more common among these patients. Predominantly among SOT recipients, infection occurred late after transplant (>12 months), and none of the infections were catheter-associated (2).
For systemic infections, monotherapy might result in emergence of resistance. In a report from Taiwan, 3 of 7 R. equi isolates had inherent concomitant resistance to all β-lactams, macrolides, and rifampin (9). We did not observe this multidrug-resistance pattern. For transplant recipients with systemic infection, we recommend combination treatment with 2-3 antimicrobial drugs (vancomycin, fluoroquinolone, or carbapenem). TMP/SMX and clindamycin should be avoided in empiric treatment regimens because of variable rates of susceptibility. Macrolide antimicrobial drugs, except for azithromycin, decrease the metabolism of cyclosporine, tacrolimus, sirolimus, and everolimus. Conversely, rifampin increases the metabolism of these drugs. These interactions should be considered when treating transplant recipients. Rhodococcus spp. can also form adherent biofilms (10). Thus, removal of central catheters is imperative in the management of infected patients.
We observed only 1 death attributable to infection in an HSCT recipient. This finding differs from an attributable mortality rate of 34.3% in a multicenter study of 67 patients with AIDS (mean CD4 cell count 35/µL) (11). The higher mortality rate probably reflects the degree of immunosuppression among patients with advanced HIV infection and close clinical monitoring of transplant recipients, which enables timely management. Death and relapse rates in our series were comparable with those in a review of 30 cases (2).
Our retrospective study had inherent limitations related to collection of data. In the analysis, we included SOT and HSCT recipients who differed in underlying disease states and immunosuppression. The study was also limited by the relatively small number of cases. This limitation was also reflected in wide CIs in risk factor analysis. However, we showed that risk factors for Rhodococcus infection were diabetes mellitus and recent opportunistic infection. Outcomes were generally favorable after appropriate and timely treatment.