Enterovirus D68 Infection, Chile, Spring 2014

To the Editor: Enterovirus D68 (EV-D68) is an emergent viral pathogen associated with severe respiratory illness, especially in children with asthma (1). The ongoing epidemic in the United States has expanded to 47 states; as of November 25, 2014, a total of 1,121 persons were affected (http://www.cdc.gov/non-polio-enterovirus/outbreaks/EV-D68-outbreaks.html).

FilmArray Respiratory Panel, which does not distinguish enterovirus from rhinovirus.
We sent 6 nasopharyngeal samples from the children to the US Centers for Disease Control and Prevention for detection of EV-D68 by real-time reverse transcription PCR (http://www.cdc.gov/non-polio-enterovirus/dowloads/ ev-d68-rt-pcr-protocol.pdf) and sequencing (2). Of the 6 patients, EV-D68 was confirmed for 2 patients.
Patient 1 was a boy, 7.5 years of age, who was hospitalized on September 21, 2014, with a history of asthma since 3 years of age. During the previous 3 months, his father had frequently traveled to the United States. The patient's current episode began with upper respiratory symptoms and low-grade fever (38°C) and was followed by intense vomiting. On admission, the child exhibited respiratory distress and an oxygen saturation of 88%, which led to his admission to the pediatric intensive care unit and management with noninvasive mechanical ventilation (bilevel positive airway pressure) for 48 hours. He was discharged home after 5 days of hospitalization, having required supplemental oxygen for 4 of those days.
Patient 2 was a 9-year-old boy who was hospitalized on October 1, 2014, with a history of severe asthma since 7 years of age. He had visited the emergency department multiple times for asthma crises. Both parents and his 2 brothers also had asthma. The episode reported here began 4 days before hospitalization, with a dry cough and progressive breathing difficulty, requiring 3 emergency department visits. During the third visit, low oxygen saturation (91%) led to hospitalization. The child had no fever during this entire episode. He received up to 50% fraction of inspired oxygen, intravenous methylprednisolone, intravenous magnesium sulfate, and inhaled salbutamol; he was discharged in good medical condition after 8 days of hospitalization, having received supplemental oxygen for 7 of those days.
These 2 EV-D68-positive patients had marked pulmonary hyperinsufflation and required prolonged oxygen   ‡Febrile with seizure (n = 1), fever of unknown origin (n = 1), convulsion in child with epilepsy (n = 1). §In 2014, one child was transferred to another hospital, and duration of hospital stay is unknown.
therapy. Sequence analysis of the viral protein 1 gene revealed that both of these viruses clustered with the major outbreak strain from the United States. Partial gene sequences of viral protein 1 were deposited in the GenBank database under accession nos. KP247599 and KP247600. We next used CLART PneumoVir to retest samples that had been positive for enterovirus/rhinovirus by Fil-mArray during September-October 2013 and 2014. The number of overall samples tested for respiratory viruses did not increase from 2013 to 2014 (227 and 218, respectively), but the percentage of enteroviruses detected increased strikingly (from 2.6% to 14.6%). We then compared clini-  (Table). A substantial proportion of patients hospitalized in 2014 required oxygen support and admission to the pediatric intensive care unit.
In conclusion, we report 2 confirmed cases of EV-D68 in a Southern Hemisphere country during the 2014 outbreak reported in the United States. That these cases are virologically and clinically related to those reported in the United States documents that the virus had been introduced to the Southern Hemisphere during the spring of 2014. A substantial increase in enterovirus cases displaying a notably similar clinical pattern (asthmatic crisis in children) strongly suggests that EV-D68 infections are increasingly rapidly. This virus has been previously identified in the region (3) but only sporadically. The virus could spread to other areas in Santiago and to other cities, and similar situations could occur in other Latin American countries, especially those with many residents who travel to the United States. Public health officials need to be notified of this potential, and appropriate surveillance and treatment strategies need to be implemented.