Diagnosis of Rickettsioses from Eschar Swab Samples, Algeria

To the Editor: Tick-borne rickettsioses are zoonoses caused by intracellular bacteria belonging to the spotted fever group rickettsiae (1). The main clinical signs are high fever, maculopapular rash, and an inoculation eschar at the site of the tick bite (corresponding to the portal of entry of rickettsiae into the host). Recently, several rickettsioses were diagnosed by using swab samples from skin lesions (2,3). In an animal model, as long as eschars were present, rickettsial DNA was detected (2). Our aim was to evaluate the advantage of skin swab samples for diagnosis of rickettsial diseases in a country where rickettsioses are endemic (4).


Diagnosis of Rickettsioses from Eschar Swab Samples, Algeria
To the Editor: Tick-borne rickettsioses are zoonoses caused by intracellular bacteria belonging to the spotted fever group rickettsiae (1). The main clinical signs are high fever, maculopapular rash, and an inoculation eschar at the site of the tick bite (corresponding to the portal of entry of rickettsiae into the host). Recently, several rickettsioses were diagnosed by using swab samples from skin lesions (2,3). In an animal model, as long as eschars were present, rickettsial DNA was detected (2). Our aim was to evaluate the advantage of skin swab samples for diagnosis of rickettsial diseases in a country where rickettsioses are endemic (4).
From July 2009 through October 2010, a total of 39 patients in the infectious disease department of Oran Teaching Hospital, Algeria (27 men, 12 women; median age 46.5 years) were included in a prospective study. The mean (± SD) interval between onset of lesions and consultation was 7 ± 1.8 days. Underlying conditions were present in 13 (33%) patients: diabetes (4 patients, 10.2%), hypertension (2 patients, 5%), chronic renal failure (1 patient, 2.5%), cervical cancer (1 patient, 2.5%), bronchial cancer (1 patient, 2.5%), and tobacco consumption (6 patients, 15%). Fever and generalized maculopapular rash (also on palms and soles) were found for 38 (97.4%) patients, including 5 (12.8%) with purpuric rash. One (2.5%) patient had 2 eschars on the back. A dry sterile swab (Copan, Brescia, Italy) sample was collected from the inoculation eschar of each patient by the same person (N.M.). Two patients had 2 swab samples collected from the same eschar. Only 1 swab sample was collected from the patient with 2 eschars. Scabs were removed from eschars before swabbing. The swabs, while being rotated vigorously, were directed to the base of the eschar at a 50°-60° angle for 5-6 times. For 4 patients, an eschar biopsy sample was collected under sterile conditions. Swabs were then placed back in their tubes and stored at -20°C before transportation to Unité des Rickettsies, Marseille, France.
In the laboratory, each sample was placed in 2 mL of culture medium. DNA was extracted from or skin biopsy samples by using the QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions, with a fi nal elution volume of 100 μL. We used quantitative real-time PCR (qPCR) to determine quality of DNA extraction and level of housekeeping gene coding for β-actin (5) and to detect rickettsiae (2,5). Mean (± SD) cycle threshold (C t ) value of the β-actin gene for all swab samples was 24.9 (± 2.7). Of 41 swab samples, 26 were positive for rickettsial DNA (63.4%) by qPCR targeting the RC0338 gene (5); mean C t value was 33.99 (± 2.15). Specifi c Rickettsia conorii conorii qPCR, targeting the putative acetyltransferase gene (2), had positive results for 25 (64%) patients; C t values ranged from 31.02 to 38.63. This sensitivity is comparable to that of PCRs for detecting R. conorii, the agent of Mediterranean spotted fever, on skin biopsy samples (4,6). Mean C t value for β-actin gene amplifi cation of R. conorii conoriipositive swab samples was 23.84 (± 2.19), signifi cantly lower than that for R. conorii conorii-negative samples (26.84 ± 2.72; p = 0.0003). Of 4 patients for whom swab and skin biopsy samples were available, 3 had positive results.
Opinions of health care providers and patients were evaluated by using standard questionnaires (Table). Most health professionals preferred collecting swab samples over biopsy samples for patients and for themselves (46 vs. 5 and 57 vs. 2, respectively; p = 0.0001). Patients from France and Algeria also preferred having a swab sample taken over a skin biopsy sample (43 vs. 7; p = 0.0001). Statistical analyses were conducted by using GraphPadPrism version 2.0 (www.graphpad.com/prism/Prism. htm) to p<0.5.
Swabbing an eschar is a rapid and simple technique that can be easily performed without risk for the side effects associated with biopsy sampling. Insuffi cient material taken during swabbing, evidenced by high C t values of β-actin, results in low rickettsial load, explaining the falsenegative results. This test can be used at the bedside or in an outpatient clinic and could be useful for epidemiologic and clinical studies. Because qPCR results can be obtained <4 hours after sampling, this technique might be useful for point-of-care diagnosis. The opinions expressed by authors contributing to this journal do not necessarily refl ect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affi liated.