Clonal Spread of Streptococcus pyogenes emm44 among Homeless Persons, Rennes, France

To the Editor: Streptococcus pyogenes, or group A streptococci (GAS), are human pathogens responsible for pharyngitis as well as skin and soft tissue infections. Invasive GAS diseases, including bacteremia, cellulitis, and necrotizing fasciitis, are life-threatening, especially when associated with toxic shock syndrome. Several risk factors for GAS infections are known, such as diabetes, immunosuppression, drug use, and skin lesions (1,2).

To the Editor: Streptococcus pyogenes, or group A streptococci (GAS), are human pathogens responsible for pharyngitis as well as skin and soft tissue infections. Invasive GAS diseases, including bacteremia, cellulitis, and necrotizing fasciitis, are life-threatening, especially when associated with toxic shock syndrome. Several risk factors for GAS infections are known, such as diabetes, immunosuppression, drug use, and skin lesions (1,2).
In France in 2008, 12% of GAS strains were reported resistant to tetracycline by the national reference center. Unexpected recognition of 8 tetracycline-resistant GAS isolates in January and February 2009 at the 1,950-bed University Hospital of Rennes in western France led to further investigation. We report results of characterization of tetracycline-resistant GAS isolates collected during 2009 from hospitalized and outclinic patients.
Isolates were identifi ed as GAS on the basis of β-hemolysis, Gram staining, negative catalase test result, positive pyrrolidonyl arylamidase test result, and agglutination with Lancefi eld group A antiserum. Antimicrobial drug susceptibility to penicillin G, amoxicillin, erythromycin, lincomycin, tetracycline, rifampin, streptomycin, kanamycin, gentamicin, and vancomycin was tested by using the disk diffusion method according to the criteria of the French Society for Microbiology (www.sfm.asso.fr). Of 72 nonduplicate GAS isolates collected, 25 (17 from inpatients, 8 from outpatients) were identifi ed as tetracycline resistant; they were further characterized as described (3).
The emm types of these 25 tetracycline-resistant strains were determined by sequencing the variable 5′ end of the emm gene and comparing sequences with the Centers for Disease Control and Prevention database (www.cdc.gov/ncidod/biotech/ strep/doc.htm). Twenty-three strains were emm44 type, 1 was emm105, and 1 emm83. Pulsed-fi eld gel electrophoresis (PFGE) patterns obtained after DNA digestion by SmaI restriction enzyme were compared according to Tenover criteria (4). The epidemic clone including 22 strains was characterized by an identical PFGE pattern 44-A1, whereas PFGE pattern 44-A5 of the remaining emm44 strain differed by 4 DNA bands (Figure). Epidemic strains also shared the same biotype 3 obtained on rapid ID 32 Strep strips (bioMérieux, Marcy l'Etoile, France). T types were determined on trypsinated bacteria by slide agglutination with type-specifi c antisera. Eleven strains were type T11, 4 type T11/12, 1 type T11/3/13/B3264, and 6 non-T-typeable.
All epidemic emm44 strains were susceptible to all antibacterial agents tested except tetracycline. MICs of tetracycline, determined with Etest method (AB Biodisk, Solna, Sweden), were 24-48 mg/L. Screening of strains by PCR for tet(M), tet(O), tet(K), and tet(L) genes showed tetracycline resistance was related to tet(M) gene. A multiplex PCR for detection of speA, speB, speC, smeZ, and ssa toxin genes showed that epidemic strain possessed only speB gene.
Investigation conducted by local health authorities showed that the fi rst 5 patients with emm44 strain were drug users sharing a squat (illegally occupied housing). Although this place was shut down at the end of February after an outbreak of scabies, additional cases of infections caused by emm44 strain occurred. Medical care is diffi cult to implement for homeless persons, thus, we limited our action to swabbing symptomatic persons to treat them and to limit spread of the epidemic strain. Following recommendations from the Institute for Public Health Surveillance, in mid-April nurses at the 2 main social centers for homeless persons obtained samples from 17 persons. Eleven persons were infected with GAS, of whom 8 had not been swabbed before. All but 1 isolate was emm44.
We report clonal spread of an emm44 tetracycline-resistant GAS strain in marginal populations (drug users and homeless persons) in Rennes. This strain, characterized by PFGE pattern 44-A1, represented 22/25 tetracycline resistant GAS isolates and 30% of the 72 GAS isolates identifi ed at the hospital in Pontchaillou in 2009. Locally, emergence of the 44-A1 clone led to the dramatic increase of GAS tetracycline resistance, from 17% in 2008 to 35% in 2009. emm44 GAS strains, which share identical 5′emm sequences with previously designated M/ emm61 strains (5), have mainly been isolated in Asia from throat and skin specimens (6,7). They were rarely reported as responsible for invasive infections in France or other parts of the world (5,8). Polyclonal and emm25 and emm83 monoclonal GAS outbreaks have been recently described among drug users in Switzerland, the United Kingdom, and Spain (9,10) without robust evidence of enhanced virulence of the causative GAS strains.
In the outbreak we report, skin infections might be a leading cause of bacterial transmission between people living in poor hygienic conditions and overcrowded spaces.