Limited Susceptibility of Chickens, Turkeys, and Mice to Pandemic (H1N1) 2009 Virus

To determine susceptibility of chickens, turkeys, and mice to pandemic (H1N1) 2009 virus, we conducted contact exposure and inoculation experiments. We demonstrated that chickens were refractory to infection. However, oculo-oronasally inoculated turkeys and intranasally inoculated mice seroconverted without clinical signs of infection.

A second transmission experiment was performed with the same contact exposure experimental setup, which included 8 infected pigs and 5 turkeys (4 weeks of age). In addition, 28 chickens (1 week of age) and 28 turkeys (1 week of age) were inoculated with 200 μL of virus suspension (10 6 TCID 50 ) directly into the left air sac. Six fattening turkeys (16 weeks of age) from a local fl ock were inoculated oculo-oronasally with 10 6 TCID 50 of A/Regensburg/ D6/09 (H1N1) virus. Six fattening turkeys were inoculated oculo-oronasally with 10 8 TCID 50 of the more recently isolated pandemic (H1N1) 2009 A/Bayern/74/2009 virus. Cloacal and oropharyngeal swab samples from poultry infected oculo-oronasally, through the air sac, or by contact with pigs were collected daily. Samples were tested by using a real-time reverse transcription-PCR with subtype H1N1-specifi c primers (http://offl u.net) (Table).
None of the inoculated animals became ill after infection by any tested route; the intravenous pathogenicity index of the virus for chickens was 0. All swab samples from poultry after contact with infected pigs or from poultry inoculated through the air sac were negative for virus (Table). Virus excretion by pigs was detected (3); real-time reverse transcription-PCR cycle threshold values >26 (contact experiment with chickens) or >17 (contact experiment with turkeys). Virus RNA was detected (cycle threshold values 27-39) in swab samples obtained 1-6 days postinoculation from the oropharynx of turkeys inoculated with 10 8 TCID 50 of the A/Bayern/74/2009 (H1N1) strain. Small amounts of virus RNA also were detected in the lung and left air sac from a few chicks and poults early after infection through the air sac (Table), which most likely represent residual inoculum. Although contact exposure pigs were infected (3), seroconversion was not detected in any of the tested contact exposure poultry species. Poultry inoculated intravenously or through the air sac and chickens inoculated oculo-oronasally were negative for antibodies against infl uenza A virus nucleoprotein.

Conclusions
Our results demonstrate lack of susceptibility of chickens and minor susceptibility of turkeys for infection by pandemic (H1N1) 2009 virus. Transmission of swine infl uenza viruses to poultry, particularly to turkeys, has been demonstrated (5), and experimental infection of chickens with virus subtype H3N2 resulted in a low replication rate, primarily in the alimentary tract (6). In contrast, our data indicate that pandemic (H1N1) 2009 virus cannot productively infect chickens at the ages of 1 week (air sac inoculation), 12 weeks (contact exposure, intravenous inoculation), or 15 weeks (oculo-oronasal inoculation), or turkeys at the ages of 1 week (air-sac inoculation) and 4 weeks (contact exposure) because neither virus excretion nor seroconversion was observed during the 10-day observation period. Fattening turkeys from a local fl ock seroconverted after oculo-oronasal inoculation, but virus RNA was detected in respiratory samples only in turkeys inoculated with a high dose of the A/Bayern/74/2009 (H1N1) virus (Table).
On the basis of our experiments, risk for transmission of pandemic (H1N1) 2009 virus strain to chicken and turkeys and subsequent possible reassortment with other avian infl uenza viruses should be low. However, we observed a slightly higher susceptibility of older turkeys to high doses of pandemic (H1N1) 2009 virus, which is consistent with recent reports of infected layer turkey fl ocks in Chile and Canada (7,8). Analysis of specifi c virus strains involved in these outbreaks would be useful for confi rming these observations and analyzing different strains. Host factors (age, physiologic state, stress levels, and concurrent infections) infl uencing susceptibility of turkeys should be investigated. Our data, which demonstrate absence of illness in poultry after inoculation with pandemic (H1N1) 2009 virus, are consistent with those of recent experimental infections of poultry, including turkeys (9)(10)(11). Seroconversion was detected only in turkeys by using a hemagglutinationinhibition test in 1 study (9), which might indicate higher sensitivity of competitive ELISAs used in our study.
As in other studies (12,13), characterization of pandemic (H1N1) 2009 virus strains in BALB/c mice showed differences in lethal dose and clinical signs dependent on the virus strain. None of the infected BALB/c mice in our study showed clinical signs. These fi ndings may have resulted from the fact that mice were infected intranasally without anesthesia and with a dose <10 6 TCID 50 /animal or because of a different phenotype of the virus strain used. However, replication competence of pandemic (H1N1) 2009 virus in mice without prior adaptation was indicated by seroconversion, at least for the higher infectious doses (Table). Intraperitoneal inoculation did not cause development of infl uenza virus-specifi c antibodies. This fi nding cannot be explained by receptor specifi city of pandemic (H1N1) 2009 virus because Childs et al. (14) showed that representative pandemic (H1N1) 2009 viruses bound not only to most α2-6-linked sialyl moieties, irrespective of the backbone chain length and type, but also to a considerable range of α2-3-linked sialyl residues. Although virus rep-  lication may be reduced after intraperitoneal inoculation, development of neutralizing antibodies after inoculation by this route has been reported (15).
Our results demonstrate lack of susceptibility and absence of serologic responses of chickens and turkeys to contact infection with an early human-origin isolate of pandemic (H1N1) 2009 virus. However, direct inoculation of fattening turkeys resulted in seroconversion and detection of virus RNA in oropharyngeal swab samples. Generation of new variants and reassortants caused by double infections with other infl uenza A viruses and pandemic (H1N1) 2009 virus in poultry other than turkeys appears unlikely. However, further adaptation of pandemic (H1N1) virus strains in turkeys cannot be excluded.