Serologic Survey of Pandemic (H1N1) 2009 Virus, Guangxi Province, China

Suggested citation for this article: Chen H, Wang Y, Liu W, Zhang J, Dong B, Fan X, et al. Serologic survey of pandemic (H1N1) 2009 virus, Guangxi Province, China [letter]. Emerg Infect Dis [serial on the Internet]. 2009 Nov [date cited]. Available from http://www.cdc.gov/EID/content/15/11/1849.htm


Serologic Survey of Pandemic (H1N1) 2009 Virus, Guangxi Province, China
To the Editor: Since mid-April 2009, a new infl uenza A virus (H1N1), now called pandemic (H1N1) 2009 virus, has caused infl uenza outbreaks in humans in North America (1) and a worldwide pandemic (2)(3)(4). Human pandemics occur when a new virus subtype emerges that is capable of human-to-human transmission in a population with little or no neutralizing antibodies to the new virus (4).
The current outbreak presents the fi rst opportunity to directly observe this process. We used hemagglutination inhibition (HI) and virus neutralization (VN) assays to detect antibodies in 4,043 serum samples from residents (7-84 years of age) of 2 counties in Guangxi Province, People's Republic of China, collected during July-August 2008. These persons were mostly farmers who lived in rural areas. Serum samples were obtained, transported, and frozen at -80°C as described (5). No participants had a history of vaccination against seasonal infl uenza. Antibodies were also detected in another 22 persons (<40 years of age) in Shantou, Guangdong Province, who had received 3 vaccinations for seasonal infl uenza since 2006.
Infl uenza viruses used in this study were A/California/04/2009 (H1N1; CA04), A/Brisbane/59/2007 (H1N1; B59), and A/swine/Hong Kong/915/ 2004 (H1N2; Sw915). CA04 and B59 were kindly provided by the World Health Organization Collaborating Centers for Reference and Research on Infl uenza (Atlanta, GA, USA, and Parkville, Victoria, Australia). Sw915 was isolated from pigs by our laboratory. Seven of 8 genomic segments of Sw915 were located in a sister lineage to the current outbreak; this strain is the most closely related swine virus to CA04 identifi ed to date (6). All serum samples were treated with a receptordestroying enzyme and absorbed with fresh turkey erythrocytes to remove nonspecifi c inhibitors before the assays. All samples were tested by HI and VN assays according to standard protocols (5).
Screening by HI assay showed that 70 samples were positive (titers >40) for CA04 (Table). Examination by VN assay showed that of 70 HIpositive serum samples, 12 had detectable neutralizing antibodies to CA04 (positive rate 0.3%). Of these VN-positive samples, 10 had titers of 40-80 and only 2 had neutralizing antibody titers >160 (Table). The 12 persons from whom the samples were obtained were 30-60 years of age. In contrast with fi ndings from a recent serologic survey of a US population (7), our results showed that none of the 583 persons >60 years of age in our study was VN seropositive for CA04.
All 70 HI-positive samples for CA04 were also screened for neutralizing antibodies against Sw915. Thirteen samples collected from persons 40-84 years of age were VN positive (titers 40-160). Of these 13 samples, 5 were positive (VN titer >40) for CA04 and 8 were negative. However, 7 CA04 VN-positive samples were negative for Sw915. These fi ndings suggest that some cross-reactivity exists between CA04 and other Sw915-like H1 subtype viruses circulating in the pig population in southern China, and that sporadic human infection with H1 swine viruses has occurred in rural China, where exposure to pigs is common.
In contrast, screening all 4,043 serum samples with A/Brisbane/59/2007 showed that 159 (3.9%) samples had HI titers >40, of which 116 (2.9%) had neutralizing antibodies (titer >40) (Table). Only 3 serum samples from persons >60 years of age were VN positive for B59. Because the study group was not vaccinated, these results likely refl ect natural infection rates for seasonal infl uenza virus (H1N1). The LETTERS 22 serum samples from vaccinated persons had no neutralizing antibodies against CA04, but all had high seroconversion rates for B59 (Table).
Our results suggest that most persons in our study population from Guangxi, China, are seronegative for pandemic (H1N1) 2009 virus (1). Serum samples from only 0.3% of persons tested neutralized the novel CA/04 strain. This fi nding contrasts with fi ndings from the United States that serum samples from ≈11% of unvaccinated persons had antibodies against CA04 (7). Furthermore, all CA04-positive persons in our study were <60 years of age; the US study reported a 33% seropositive rate for this age group.
These differences may have been caused by the high proportion of seasonal infl uenza vaccination coverage in the United States when compared with results form our unvaccinated population from southern China. Therefore, we suggest that vaccination against seasonal infl uenza, rather than exposure to older, seasonal, infl uenza viruses (H1N1), which may be genetically and antigentically similar to pandemic (H1N1) 2009 virus, as suggested (7), might have generated partial protection against this new virus. No persons in our vaccinated control group had neutralizing antibodies against CA04.
We hypothesize that the absence of neutralizing antibodies in our con-trol group, all of whom had been vaccinated 3 times, suggests that prolonged and repeated vaccination is required for partial immunity to CA04 or that older vaccines may confer some degree of protection. If these serologic differences are indicative of increased susceptibility, we would expect higher infection attack rates in largely unvaccinated populations than in vaccinated populations in countries such as China.