Salmonella bongori 48:z35:– in Migratory Birds, Italy

To the Editor: Serovars of Salmonella, other than subspecies enterica, are primarily associated with cold-blooded animals and infrequently colonize the intestines of warm-blooded animals. Strains of S. bongori, in particular, are rarely isolated from human patients (1,2). However, during 1984–2004, 27 strains of S. bongori with the antigenic formula 48:z35:– were identified from 2 epidemic clusters in Italy (Messina, October 1984–May 1985, and Palermo, July–October 1998), and from sporadic cases of acute enteritis that occurred in several cities in Sicily. Almost all cases involved children 1 month to 3 years of age (3). By 2006, 8 additional isolates of the same serovar had been collected in southern Italy from the following sources: 1) a healthy human carrier; 2) 3 warm-blooded animals (2 apparently healthy pigeons and a dog with diarrhea); 3) 2 food products (soft cheese and the shell of a hen’s egg); and 4) urban wastewater (3).

Until now, the rare S. bongori 48:z 35 :-, an apparent epidemiologic peculiarity of Sicily, could not have been traced to a well-defi ned source. Indeed, in past years, many infrequently isolated and new serovars of Salmonella have been identifi ed in Sicily from wild reptiles, but S. bongori 48:z 35 :-has never been isolated from these animals (5). Moreover, whether pigeons that live in urban areas have epidemiologic importance as sources of infection is questionable, because of the apparently exclusive infection of infants in the fi rst months of life. The role of migratory birds has not previously been assessed.
To determine the prevalence of birdborne pathogens in the migratory bird fauna of the Mediterranean basin, we conducted a study in October 2006, during ringing (banding) activity at the station of the University of Palermo on Ustica, a small island in the Tyrrhenian Sea (38°42′N, 13°11′E) near the northern coast of Sicily. Apparently healthy birds trapped by mist nets during active migration were sampled. Fresh fecal samples or cloacal swabs from 239 birds belonging to the orders Passeriformes, Gruiformes, and Caprimulgiformes were analyzed to determine whether birds were colonized with Salmonella. Routine procedures for isolation of Salmonella spp. were used. Colonies with morphologic characteristics of Salmonella spp. were fully identifi ed by standard biochemical and serologic testing.
Two isolates, from 2 blackcaps (Sylvia atricapilla), were identifi ed as Salmonella spp. Morphologic features of the external fl ight apparatus and weight clearly indicated that the 2 individuals belonged to the migratory subspecies of blackcap (6).
The Salmonella isolates were characterized by serotyping at the Centre for Enteric Pathogens of Southern Italy, University of Palermo, as S. bongori 48:z35:-. Molecular typing by PFGE after digestion of DNA by XbaI showed a banding pattern similar to that of all previously identifi ed S. bongori 48:z35:-. In particular, the DNA restriction pattern proved to be indistinguishable from patterns of the human isolates belonging to the epidemic clusters and of the pigeon isolates (3).
Previous studies have documented that carriage of Salmonella spp. by apparently healthy migrating birds is infrequent, although some isolates have been recovered (7,8). Prevalence has been shown to be higher during breeding season and during wintering in some urban-associated bird species, especially in those that feed on refuse, such as corvids and gulls (9). However, to our knowledge, S. bongori in migratory birds has not previously been reported.
Our fi ndings suggest that passerine migratory birds may play a role in the introduction or persistence of S. bongori 48:z35 in southern Italy. The bacteria also have the potential for gaining access to the food chain, as confi rmed by their presence in the shell of hen eggs. Moreover, a birdenvironment-food network could perpetuate a reservoir of S. bongori 48:z35:-. Most small passerine migratory birds, including blackcaps, do not share a niche with humans and are most likely to be found in rural habitats (10). However, some species are developing an increasing ability to live in urban and suburban environments, especially where winter feeding by humans attracts birds, forcing changes in the species balance. Because of their ability to fl y through long distances during annual migrations, wild birds could also play a role in the epidemiology of zoonoses. Thus, risk assessment of Salmonella carriage in wild birds warrants further investigation.

Comet Sign (and Other) in Pyemotes Dermatitis
To the Editor: Recently, Pascal Del Giudice et al. published an interesting article (1) about dermatitis in France caused by Pyemotes ventricosus in which they highlight the presence of the comet sign in a number of their patients. It is, they assert, a sign that because of its peculiarity could be useful for diagnosing this type of dermatitis in outbreaks and sporadic cases.
Some years ago, we studied 3 outbreaks (with >100 cases) of dermatitis caused by P. ventricosus parasitic mites in Castellón, Spain, produced by different infected materials (2). When we published the results, we concentrated on the epidemiologic characteristics and the discovery of the mite; perhaps we paid too little attention to the appearance of the lesions, of which we did not provide images. Nevertheless, we also observed the descriptions by Del Giudice et al., which we now show in the Figure