Underreporting of Human Alveolar Echinococcosis, Germany

Underreporting of Human Alveolar Echinococcosis, Germany


The Study
The capture-recapture method estimates unascertained cases by comparing data from >2 different sources. It requires that persons have a correct diagnosis and equal probability of inclusion (catchability) and that the study population be closed. If only 2 sources are used, these should be independent (12).
We used 3 data sources: RKI, EER, and a pathologists' survey (PAS) conducted in June 2006 among all registered pathology laboratories in Germany (≈525). Pathologists were requested to complete a questionnaire reporting all echinococcosis cases diagnosed from 2003 through 2005 to RKI.
We defi ned confi rmed AE case-patients as persons with positive results of histopathologic examination or with liver lesion showing typical morphologic features, identifi ed by imaging techniques. Only case-patients with a fi rst diagnosis from 2003 through 2005 were included. Because reporting of AE is anonymous, we used 3 proxy matching identifi ers. Matching criteria were identical: 1) year and month of birth, 2) sex, and 3) year and month of diagnosis (±6 months to allow for time variability of different diagnostic methods). For case-patients for whom month of birth or month of diagnosis was missing in >1 source, the fi rst 3 digits of the case-patient's postal code or the referring physician's postal code had to be identical in addition to the above criteria.
The distribution of matched and unmatched observed cases by source is displayed in a Venn diagram (Figure). To predict the frequency of unascertained cases, we constructed log-linear models. Each model included a variable for each source and up to 3 possible interaction variables between sources. The saturated model included all 3 interactions, whereas the independent model assumed no interactions (13).
We selected the fi nal model using Akaike's Information Criterion (AIC), which indicates how well a model fi ts the data, considering the number of variables included. Small values of AIC correspond to a better adapted model (12). Ninety-fi ve percent goodness-of-fi t confi dence intervals (95% CI) were calculated based on the likelihood ratio, to allow asymmetric intervals and avoid underestimation of the upper and lower limits (14).
The sensitivity of RKI data was estimated by dividing the number of cases reported to RKI by the total number of cases (N) from the selected model. Analysis was performed with Stata 9.0 (StataCorp, College Station, TX, USA) (15).
A total of 60 confi rmed cases were reported to RKI; EER registered 59. The response rate for PAS was 64% (335 of 525 surveyed). Pathologists reported 49 AE cases in the survey, of which 25 were the fi rst diagnosis, 5 were previously diagnosed, and 19 had no date of fi rst diagnosis.  (Figure). Log-linear estimates for N ranged from 184 to 399 cases ( Table 2). Model 5, with the single interaction term between RKI and PAS, was selected as the best fi tting (AIC = -3.33) model. According to this model, 70 cases were missed, yielding 184 cases (95% CI 150-242) over 3 years. This corresponds to 61 cases (95% CI 50-81) annually, with an incidence rate of 0.07/100,000 persons. The lower estimate in model 5, compared with that of the independent model, suggested a negative dependence between RKI and PAS reports. Sensitivity of RKI was 33% (95% CI 25%-40%).

Conclusions
We estimated that the national surveillance system failed to detect 67% of AE cases in Germany over 3 years. Underreporting may occur for several reasons. Pathologists might be unaware of their obligation to report. Furthermore, reports almost exclusively come from microbiologic laboratories, and, consequently, case-patients who do not undergo serologic testing, or who have seronegative results, are likely to be missed. Finally, the reporting procedure is arduous because forms are detailed and must be fi rst ordered from RKI.
Capture-recapture estimates can be biased if the underlying assumptions are violated. Because case identifi cation was based on several variables, the potential for mismatching was considered small. However, the lenient criteria may have led to overmatching. Including more or fewer matching criteria had only a small effect on the estimate.
In the fi nal models, we excluded cases reported through PAS when fi rst-diagnosis status was unknown. Log-linear analysis that included these cases resulted in a higher estimate; therefore, we are confi dent that the exclusion did not overestimate the number of cases. Varying catchability can be addressed by stratifi cation. Although the sources differed with regard to geographic distribution, we considered stratifi ed analysis inappropriate due to missing postal codes for several case-patients, zero values in 1 stratum, and small numbers in general, which would increase the uncertainty around our estimate.
AE is not equally distributed in Germany, and the different geographic distribution of cases reported by PAS compared with RKI and EER indicated that PAS had missed case-patients mainly from the south. The number of histopathologically diagnosed cases was therefore likely underestimated. The importance of this for the estimated true number of AE case-patients presented here cannot be ascertained.
The negative dependence between RKI and PAS can be explained by diagnostic practices. Unpublished data from EER suggest that histopathologically diagnosed cases are less likely to have serologic test results than those without histopathologic examination. If a case-patient has had a histopathologic examination with positive results early in the diagnostic decision-making process, additional serologic testing is unnecessary, which reduces the chance of these case-patients being reported to RKI. Reporting to EER is independent of serologic testing, which could explain the greater overlap between EER and PAS than between RKI and PAS.
Despite the limitations, the study did demonstrate poor reporting of AE. To improve the national surveillance sys- Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 14, No. 6, June 2008 tem, the focus of reporting should be shifted from microbiologic laboratories and pathologists to referring physicians, who usually collate the various diagnostic results. Sustaining a surveillance system for AE in Germany is a major challenge because the disease is rare. However, a recent report on increasing human AE in neighboring Switzerland (8) underlines the importance of an effective surveillance system with adequate sensitivity to detect changes in disease incidence in order to guide strategies for prevention and control.