16S rRNA Methylase–producing, Gram-Negative Pathogens, Japan

To investigate the exact isolation frequency of 16S rRNA methylase–producing, gram-negative pathogenic bacteria, we tested 87,626 clinical isolates from 169 hospitals. Twenty-six strains from 16 hospitals harbored 16S rRNA methylase genes, which suggests sparse but diffuse spread of pan-aminoglycoside–resistant microbes in Japan.

AF550415), and later characterized and assigned as ArmA in K. pneumoniae, E. coli, Enterobacter cloacae, Salmonella enterica, and Shigella flexneri in France, Bulgaria, and Spain (10,11). Moreover, ArmA was also identified in E. coli, K. pneumoniae, E. cloacae, C. freundii and S. marcescens in South Korea, Japan, and Taiwan (5,8,9). This enzyme has also been identified in a glucose nonfermentative Acinetobacter sp. in South Korea and Japan (6,8). Quite recently, RmtD was newly identified in the SPM-1-producing P. aeruginosa strain PA0905, which was isolated in Brazil (7). In Japan, arbekacin, a semisynthetic aminoglycoside, has been approved for treatment of methicillin-resistant Staphylococcus aureus infections, and this agent is also very efficacious for gramnegative bacteria. However, 16S rRNA methylase-producing microbes can adapt to this agent, and its prescription may well be a selective pressure on the kind of microbes in the clinical environment. Thus, this investigation was conducted to determine the exact isolation frequency of 16S rRNA methylase-producing, gram-negative pathogenic bacteria in Japanese medical facilities and assess the possibility of the future prevalence of these hazardous microbes.

The Study
From September 1 to October 31, 2004, 169 medical facilities with in-house microbiology laboratories participated in this investigation. Clinical specimens were collected from inpatients and outpatients with suspected infections. Bacterial isolates that belonged to the family Enterobacteriaceae or were nonfermentors of glucose, for example, P. aeruginosa and Acinetobacter spp., were included in this study. A total of 87,626 clinical isolates were analyzed. The results are shown in Table 1.
Pulsed-field gel electrophoresis (PFGE) was performed on 9 strains of P. aeruginosa and 3 strains of A. baumannii isolated from 4 separate hospitals where 16S 16S rRNA Methylase-producing, Gram-negative Pathogens rRNA methylase genes were isolated ( Figure 2). Genomic DNA preparations from P. aeruginosa and A. baumannii were digested with SpeI and SmaI, respectively. Clonality was inferred based on the criteria of Tenover et al. (13) Two of 3 rmtA-positive P. aeruginosa strains isolated in hospital C were estimated to be the same clone. Among 4 rmtA-positive P. aeruginosa isolates recovered in hospital D, 2 different clonal lineages were observed. This finding suggests possible conjugal transfers of rmtA-carrying plasmids among genetically different strains of P. aeruginosa. Three armA gene-harboring A. baumannii identified in hospital S were obviously the same clone. These findings imply probable nosocomial transmission of 16S rRNA methylase gene-harboring strains in hospitals C, D, and S, as well as frequent conjugal transfers of plasmids carrying 16S rRNA methylase genes among gram-negative pathogenic bacterial species.
MIC determinations were performed according to the guideline of the CLSI (formerly National Committee on Clinical Laboratory Standards). All 16S rRNA methylasepositive strains were highly resistant (MICs >1,024 mg/L) of all 4,6-disubstituted deoxystreptamine group aminoglycosides (Table 3). In contrast, resistance to streptomycin and neomycin varied. Three16S rRNA methylase genenegative P. aeruginosa strains were also highly resistant to arbekacin, but the MICs of some of the 4,6-disubstituted deoxystreptamine group aminoglycosides were relatively lower (256-512 mg/L) for these strains than those for 16S rRNA methylase gene-positive strains (>1,024 mg/L). Strains harboring 16S rRNA methylase genes tended to show resistance to oxyimino-cephalosporins such as cefotaxime and ceftazidime as well, but were susceptible to imipenem. As reported for the armA-or rmtB-bearing strains, the presence of β-lactamase genes was suggested in cefotaxime-resistant strains, and indeed the bla CTX-M-14 gene was detected in several rmtB-positive strains tested in our study (data not shown). Some of these strains also demonstrated resistance to fluoroquinolones (Table 3).

Conclusions
The overall isolation frequency of 16S rRNA methylase-gene-positive gram-negative bacilli was very low (0.03%) in Japanese medical facilities in 2004, with the highest rates seen in P. aeruginosa and Acinetobacter spp. at 0.08% and 0.13%, respectively. Twenty-six bacterial isolates carrying 1 of the four 16S rRNA methylase genes were recovered from 16 (9.5%) of 169 hospitals that participated in this nationwide investigation. Of the 169 hospitals, 162 hospitals had >200 beds, accounting for 5.9% of all Japanese hospitals of similar scale. This implies that 16S rRNA methylase-producing strains might have been present in >250 Japanese hospitals during the investigation period, which in turn suggests sparse but diffuse spread of 16S rRNA methylase producers in Japan. Since several . This distribution suggests a sparse but diffuse spread of 16S rRNA methylase-producing, gram-negative pathogenic microbes in Japan. Bacterial species and type of 16S rRNA methylase identified in each hospital are shown in Table 2. armA-or rmtB-positive strains have also been isolated in European and Asian countries, and given the potential for further dissemination, nationwide identification and ongoing surveillance of these isolates should be considered by all countries. According to PFGE typing, nosocomial transmission of 16S rRNA methylase-producing P. aeruginosa and A. baumannii was suspected in 3 hospitals (hospitals C, D, and S). The banding patterns of rmtA-harboring P. aeruginosa isolated in hospitals C, D, and F were diverse, which excluded the possibility of an epidemic P. aeruginosa strain harboring the rmtA gene. Despite the observation of 2 different PFGE profiles among the 4 P. aeruginosa strains isolated in hospital D, they might share the same plasmids carrying the rmtA gene. For further characterization of genetic relations among rmtA-harboring P. aeruginosa strains, comparative analyses of plasmids and mobile elements that carry the rmtA gene (14) should also be pursued.
Nosocomial infections caused by multidrug-resistant, gram-negative bacteria have become a serious problem in clinical facilities. P. aeruginosa and Acinetobacter spp.
have been especially efficient at developing multidrug resistance against broad-spectrum β-lactams, fluoroquinolones, and aminoglycosides (3,6,7,9). The identification of armA and rmtB genes in Europe and East Asia in both human (1-11) and livestock (15; EMBL/GenBank accession no. DQ345788) populations suggests that we must pay consistent attention to prevent further global proliferation. If 16S rRNA methylase-positive bacterial isolates disseminate widely and extensively, the high level of pan-aminoglycoside resistance will undoubtedly have an impact on illness, deaths, and costs of care in both clinical and livestock-breeding environments. supported in part by a Grant-in-Aid (no. 16790318) for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. Dr Yamane is a research scientist at the National Institute of Infectious Diseases, Japan. His research interests include infection control and the molecular mechanisms of antimicrobial resistance in nosocomial bacteria.