Japanese Spotted Fever, South Korea

We describe the first case of Japanese spotted fever and the first isolate of spotted fever group rickettsia from a patient in South Korea. The isolated rickettsia from the patient was identified as Rickettsia japonica by analysis of the nucleotide sequences of 16S rRNA, gltA, ompA, ompB, and sca4 genes.

mmol/L, a leukocyte count of 8 × 10 9 /L, and a platelet count of 87 × 10 9 /L. The patient was treated with oral doxycycline (200 mg/day), but the fever persisted during the treatment. On the third hospital day, petechiae developed on the trunk and extremities, including palms and soles ( Figure 1). The leukocyte count increased to 11.2 × 10 9 /L, and the platelet count decreased further to 32 × 10 9 /L. He also showed confusion, irritability, and radiographic evidence of interstitial pneumonitis. The patient was then given azithromycin (500 mg/day intravenously) instead of oral doxycycline because of the possibility that he was infected with doxycycline-resistant O. tsutsugamushi. His fever resolved during next 5 days and he was discharged.
The  (Division of Rickettsial Diseases, Department of Bacteriology, National Institute of Health, Seoul, Republic of Korea) and was reported to be positive against R. japonica with a titer of 1,024. The convalescent-phase serum sample, taken on the 30th day after admission, was positive for antibodies against the isolated strain (Inha1) with a titer of 5,120.
To isolate the pathogen, a few drops of blood taken on the day of admission were added directly to the monolayer cultures of ECV304 cells, a spontaneously transformed cell line derived from human umbilical vein endothelial cells (obtained from S.Y. Kim) (7). After incubation for 24 h, monolayers of ECV304 were maintained in M199 medium containing 10% fetal calf serum and observed daily with an inverted microscope. The infected cells exhibited few cytopathologic changes, displaying only a few rounded cells. On day 28, IFA staining was done by using the convalescent-phase serum of the patient to visualize the rickettsiae. Many intracellular bacteria were observed inside the cells; they were not seen in the staining with control serum. From these results, we tentatively identified our isolated bacterium (strain Inha1), as a member of SFG rickettsiae.
Amplification and sequencing of the 16S rRNA, gltA, ompA, ompB, and sca4 genes were as described by Lee et al. (6). Sequences were aligned by using the multiplealignment algorithm in the MegAlign software package (Windows version 3.12e; DNASTAR, Madison, WI, USA), and phylogenetic trees were constructed by the neighbor-joining method with the MEGA program (8). Sequences were compared to the sequences with those of 16 reference strains of SFG rickettsiae (9-11). The nucleotide sequence (AY743328) of the 16S rRNA gene was identical to that of R. japonica YH. Inha1 demonstrated 16S rRNA sequence similarities of 95.9%-99.7% to the other strains of SFG rickettsiae. In the phylogenetic tree, Inha1 formed a cluster with R. japonica YH, separate from the other strains of SFG rickettsiae (Figure 2). The nucleotide sequence (AY743327) of the gltA gene of Inha1 showed a high similarity (99.8%) with that of R. japonica YH. The sequence similarities of Inha1 to the other strains of SFG rickettsiae were 93.6%-99.1%.
The nucleotide sequence of the ompA, ompB, and sca4 of Inha1 strain also showed a high similarity to R. japonica YH and the sequence similarities to R. japonica YH were 100, 99.9, and 99.9%, respectively.

Conclusions
To identify the isolate at species level, we determined the sequence of 5 genes that have been used for the phylogenetic classification of rickettsiae (9)(10)(11)(12)(13)(14). The sequences are identical or highly homologous to those of R. japonica. Although the sequence similarity of the gltA gene of Inha1 strain to R. japonica YH was 99.8%, the other 4 genes show sufficient similarity to fulfill the criteria suggested by Fournier et al. (9).
R. japonica was first isolated in Japan from human patients and ticks (15). The isolation of R. japonica in South Korea is not surprising because of the geographic proximity of South Korea to Japan. Furthermore, among 4 SFG rickettsiae detected in Korean ticks, 3 strains were highly homologous to R. japonica (6). Therefore, R. japonica may be the most dominant SFG rickettsiae distributed in South Korea, and the geographic distribution of R. japonica may be more widespread than previously known. However, other SFG rickettsiae, including R. sibirica, may be present in northeastern Asia. To clarify this issue, more strains of SFG rickettsiae must be isolated from other locations within Korea.