Bordetella petrii Clinical Isolate

We describe the first clinical isolate of Bordetella petrii from a patient with mandibular osteomyelitis. The only previously documented isolation of B. petrii occurred after the initial culture of a single strain from an environmental source.

We describe the first clinical isolate of Bordetella petrii from a patient with mandibular osteomyelitis. The only previously documented isolation of B. petrii occurred after the initial culture of a single strain from an environmental source.
A 67-year-old man visited an emergency dental clinic, where he complained of toothache in the lower right mandibular quadrant. Examination showed a root-filled lower right canine tooth that was mobile and tender to percussion. The tooth was extracted uneventfully under local anesthesia. The patient returned after several days with pain at the extraction site. A localized alveolar osteitis was diagnosed, and local debridement measures were instituted. These measures proved unsuccessful, and repeat examination showed submandibular lymphadenopathy and tenderness to palpation in the buccal sulcus in the extraction site. Radiographs showed no abnormality at this stage.
A course of oral amoxicillin (250 mg, every 8 h for 5 days) followed by oral metronidazole (200 mg every 8 h for 5 days) was prescribed. Symptoms persisted, with increasing severity of pain in the affected area, and the patient was referred to a tertiary referral center. On examination there, the patient had normal full blood count, hematinics, and glucose levels. Ultrasound examination of the submental soft tissue region did not indicate any abnormal pathology. Radiographs showed radiolucencies in the bone surrounding the extraction site. During this period a 3-week course of oral co-amoxiclav (375 mg, every 8 h) was prescribed. A bone biopsy was performed under local anesthesia, and a diagnosis of mandibular osteomyelitis was made.
A portion of the bone biopsy specimen was cultured in 10 mL Fastidious Anaerobe Broth (FAB) (BioConnections, Wetherby, UK) and incubated for 48 h at 37°C in air. After 48 h, the FAB was subcultured onto 1) Fastidious Anaerobe Agar (FAA, BioConnections), incubated for 72 h at 37°C under anaerobic conditions, and 2) Columbia Blood Agar (CBA, BioConnections), incubated for 72 h at 37°C under 5% CO 2 atmospheric conditions. Culture on both FAA and CBA showed a pure growth of a facultative gram-negative bacillus that had not been identified with routine laboratory protocols. Initial susceptibility testing using disk diffusion indicated apparent susceptibility of the isolate to erythromycin, gentamicin, ceftriaxone, and piperacillin/tazobactam. The isolate was resistant to amoxicillin, co-amoxiclav, tetracycline, clindamycin, ciprofloxacin, and metronidazole. After initial sensitivity results, a 6-week course of oral clarithromycin (500 mg, 8 hourly) was begun.
At follow-up appointments 3 months and 6 months after antimicrobial drug therapy ceased, clinical and radiographic findings were not unusual, and the infected area healed successfully. Despite the successful clinical outcome, the isolate was subsequently shown to be resistant to clarithromycin in vitro (Table). Improvement of the osteomyelitis may also have been facilitated by the biopsy procedure, during which a sequestrum of bone was removed.
The gram-negative bacillus (designated strain GDH030510) was submitted to the Health Protection Agency, Centre for Infections, London, for identification. Preliminary tests results were consistent with those described for members of the genus Bordetella. Colonies had the following phenotypic characteristics: positive reaction for oxidase and negative reaction for urease production, motility using the hanging-drop method at 37°C, and slide agglutination with B. pertussis and B. parapertussis antiserum (Difco, Shannon, Ireland). The organism could be cultured on MacConkey agar and was nonhemolytic on blood agar. Genomic DNA was extracted by using the InstaGene Purification Matrix (BioRad, Hercules, CA, USA). DNA amplification of small-subunit (SSU) rRNA genes was performed by using primers 27f and 1525r (1). Amplification and sequencing of the gene for the Bordetella outer membrane protein A (ompA) and the RisA response regulator (risA) were as described by von Wintzingerode et al. (2). Reaction mixes contained the following: 2 mmol/L MgCl 2 , 10 mmol/L Tris-HCl (pH 8.  [5]). Exclusion of these 3 bases increased the similarity to 99.5% (1,468/1,476 nt). The 445 nt of the risA gene sequence of strain GDH030510 that were determined showed a maximum similarity value of 93.9% (418/445 nt) with the risA gene from the B. petrii type strain (AJ242553). The species with the next highest similarities were B. parapertussis, B. avium, B. bronchiseptica, and B. pertussis, all with similarities of 88.3% (393/445 nt). The 414 nt of the ompA gene sequence that were determined showed maximum a similarity value of 92.0% (381/414 nt) with the ompA gene from the B. petrii type strain (AJ242599). The species with the next highest similarities were B. bronchiseptica and B. parapertussis, both with similarities of 87.9% (364/414 nt).
Further susceptibility testing was undertaken, with MICs determined by agar dilution on diagnostic sensitivity test agar (Oxoid, Basingstoke, UK) supplemented with 5% lysed horse blood, with inocula equivalent to 0.5 and 2 McFarland standards, and with incubation at 37°C in air for 24 to 36 h to ensure adequate growth. No inoculum effects were noted on MICs for any antimicrobial agents. MICs are shown in the Table. Currently, the genus Bordetella comprises 8 species (2), 7 of which have been isolated from humans and a variety of warm-blooded animals. Three of these species, B. pertussis, B. parapertussis, and B. bronchiseptica, are often referred to as the classic Bordetella species (6); they are closely related phylogenetically but have distinct host ranges. B. pertussis is an obligate pathogen for humans and is the etiologic agent of pertussis. B. parapertussis causes a similar, usually milder, infection in humans, and B. parapertussis strains may also be isolated from sheep with chronic pneumonia. Human isolates of B. parapertussis are highly clonal and appear distinct from sheep isolates. B. bronchiseptica is known to infect many mammals, including humans, although human infection is rare and usually occurs in immunocompromised hosts. B. avium is pathogenic for birds, including poultry, and causes coryza or rhinotracheitis in turkeys. Three other species of Bordetella, B. hinzii, B. holmesii, and B. trematum, have subsequently been described (7)(8)(9). B. hinzii is a commensal of the respiratory tract of fowl but has some pathogenic potential in immunocompromised humans (10) and was implicated as the causative agent in a fatal case of septicemia (11). B. holmesii has been isolated from blood of young adults, and nasopharyngeal specimens (9,12,13). B. trematum has been isolated from human ear infections and wounds, but its pathogenicity remains unknown (7). The most recently recognized species, B. petrii, was described in 2001 (2) and was isolated from an anaerobic bioreactor culture enriched from river sediment. This description was based on a single isolate and, to our knowledge, no further isolates of this species have been previously reported from any source.
The source of infection of the strain described here and the pathogenic role of B. petrii are currently unknown. However, the identification and characterization of further clinical isolates should help determine the reservoir and virulence potential of this intriguing species.

Addendum
A clinical isolate of a new species of Bordetella, Bordetella ansorpii sp. nov, has recently been reported (14). Phylogenetically, it appears to be more closely related to B. petrii than to other members of the genus.