A New Methanogenic, Hydrogenotrophic Archaeon from Spitsbergen Permafrost

V. E. Trubitsyn; Трубицын В. Э.; N. E. Suzina; Сузина Н. Е.; E. M. Rivkina; Ривкина Е. М.; V. A. Shcherbakova; Щербакова В. А.

doi:10.31857/S002636562260064X

A New Methanogenic, Hydrogenotrophic Archaeon from Spitsbergen Permafrost

Authors: Trubitsyn V.E.¹, Suzina N.E.¹, Rivkina E.M.², Shcherbakova V.A.¹
Affiliations:
1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
2. Institute of Physicochemical and Biological Problems of Agriculture, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
Issue: Vol 92, No 2 (2023)
Pages: 135-145
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://journals.rcsi.science/0026-3656/article/view/138156
DOI: https://doi.org/10.31857/S002636562260064X
EDN: https://elibrary.ru/ATFLJN
ID: 138156

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Abstract

Abstract—A new strain of methanogenic archaea, designated VT^T, was isolated from a sample of Spitsbergen permafrost. The cells were nonmotile curved rods, 2.7–5.3 × 0.3 µm. The optimal conditions for growth were 20°C, pH 6.6, and NaCl concentrations 0.03–0.05 M. The H₂/CO₂ gas mixture was the only substrate used. In the presence of H₂/CO₂, growth was stimulated by addition of yeast extract or rumen fluid. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain VT^T belonged to the genus Methanobacterium and was most closely related to M. lacus 17A1^T (97.02% similarity). Comparison of the sequenced and assembled genome of strain VT^T with the genomes of other members of this genus confirmed these results and revealed species-level differences. Our results indicate that this methanogenic isolate belongs to a new species of methanogenic archaea, for which the name Methanobacterium spitsbergensе sp. nov. was proposed, with the type strain VT^T (=VKM B-3566^T = JCM 39284^T).

Keywords

methanogenic archaea, Methanobacterium spitsbergensе, Spitsbergen, permafrost deposits, phylogenetic analysis, genome sequencing

References

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