Normal and vitamin B6-deficient rats received an intraperi-toneal injection of 30mg/100g of body wt, and the contents of metabolites in kidney or plasma and the related enzyme activities in kidney were determined. The contents of kynurenine and 3-hydroxykynurenine in B₆-deficient rat plasma and kidney were much higher than those in normal rat. The changes of those contents in plasma were parallel to those in kidney, but not in liver. The contents of kynurenic acid and xanthurenic acid in B₆-deficient liver, plasma, and kidney were also much higher than those in normal rats. However, the changes of those contents in plasma were parallel to those in liver, but not in kidney. Xanthurenic acid and kynurenic acid accumulated to a much greater extent in kidney than in plasma and liver. Kidney kynureninase activity was very low, but kynurenine aminotransferase activities were very high. These observations indicated that the production of xanthurenic acid after tryptophan injection was favorable in B6-deficient kidney with respect to enzyme activities and substrate concentrations, and suggested that kidney took up kynurenine or 3-hydroxykynurenine from blood and after conversion of them it excreted xanthurenic or kynurenic acid into urine.