Adjustable Vertical Vein Ligation in Supracardiac Totally Anomalous Pulmonary Venous Connection: A Clinical Report of 99 Patients

Received date: August 04, 2020; Accepted date: September 04, 2020; Published date: September 11, 2020 Citation: Ujjwal K. Chowdhury., Diplomate NB., Singh S., George N., Hasija S., et al., (2020) Adjustable Vertical Vein Ligation in Supracardiac Totally Anomalous Pulmonary Venous Connection: A Clinical Report of 99 Patients.. J, Clinical Cardiology and Cardiovascular Interventions, 3(8); Doi:10.31579/2641-0419/075 Copyright: © 2020 Ujjwal Kumar Chowdhury, This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction
Despite fine tuning of surgical techniques and pulmonary vasodilators, recurrent episodes of pulmonary hypertensive crises and low cardiac output remains a significant problem in repaired obstructive TAPVC leading to hemodynamic instability and mortality after surgery [1][2][3][4].
Studies have demonstrated that the left atrium lacks both normal compliance and reservoir function and the left ventricle (LV) is noncompliant and dysfunctional in a subset of patients with obstructive TAPVC [5][6][7][8][9][10][11]. It is postulated that a patent vertical vein may function as a temporary reservoir for pulmonary venous blood after repair of TAPVC, permit unloading of the volume in the small, non-compliant left-sided cardiac chambers until they are able to grow and adapt to increased flow demands [5,6,12,13]. Not all investigators have accepted these findings or utilized these techniques. Clinical studies on an unligated vertical vein in the setting of obstructive TAPVC are limited and insufficient to generate evidence-based guidelines.
In our previous investigation on ligation versus non ligation, we demonstrated that unligated vertical vein in repaired obstructive TAPVC reduces perioperative pulmonary artery pressure, pulmonary hypertensive crises and improves survival [14].
Subsequently, in 2007, we demonstrated that a percutaneously adjustable vertical vein ligature allows gradual tightening of the ligature under optimal physiologic conditions without reopening the sternum 15 . In the current study, we did not repeat the above analysis.
The primary objective of this study was to i) determine the short-and longterm impact of delayed vertical vein ligation using an adjustable vertical vein ligature on survival of patients with supracardiac TAPVC having moderate or severe pulmonary artery hypertension. The secondary objectives of the study were to: ii) examine whether the unligated vertical vein is serving as a temporary "pop-off" valve in the event of pulmonary hypertensive crises; iii) ascertain the timing to occlude the vertical vein; iv) evaluate the presence of iatrogenic kinking of left superior pulmonary vein and left brachiocephalic vein, if any and v) study the requirement of cardiac reoperations for residual shunts at the level of vertical vein or recurrent pulmonary venous obstruction.

Methods
This retrospective study conforms to the principles outlined in the declaration of Helsinki and was approved by the Institutional Ethics Committee. Patients were enrolled in the study protocol after obtaining informed written consent from patients/parents/guardians.

Patient selection criteria
This study evaluated short-and long-term outcomes after delayed ligation of the vertical vein in a consecutive series of patients undergoing repair of isolated supracardiac TAPVC.

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Only patients with isolated supracardiac TAPVC with a discernible ascending vertical vein were included.

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All patients with anomalous pulmonary venous drainage to the right SVC, coronary sinus or right atrium (RA), those with infracardiac and mixed TAPVC or associated complex congenital cardiac malformations and heterotaxy syndromes were excluded.
 All patients with obstructive TPAVC underwent adjustable vertical vein ligation.
 Patients with non-obstructive TAPVC with moderate to severe pulmonary arterial hypertension (PAH) and hemodynamic decompensation, requiring inotropes and ventilator support in the preoperative period underwent adjustable vertical vein ligation ( Figure 2)  In patients with non-obstructive TAPVC, without preoperative PAH or mild PAH, snaring of vertical vein was done after coming off cardiopulmonary bypass and systolic PAP (SPAP) and mean LAP (MLAP) were measured. If SPAP ≥31 mmHg, MLAP ≥18mmHg, and peak systolic right-to-left ventricular pressure ratio >1.0, adjustable vertical vein ligation was performed. Otherwise vertical vein was directly ligated and those patients were excluded from the study.
. , either isolated or in combination in the operating room or in the intensive care unit, to maintain stable hemodynamics in the absence of residual structural lesions and mechanical external compression after correction of all electrolytes or blood gas abnormalities and after adjustment of the preload to its optimal value. Low-output syndrome was also diagnosed if there was an increasing requirement of the previously mentioned inotropes along with afterload reduction with sodium nitroprusside. Patients who received less than 4 µg/(kg/min) dopamine to increase renal perfusion were not considered to have low output syndrome.
Invasive monitoring to measure cardiac output directly (thermodilution catheter, PA pressure line, and thermistors) is cumbersome and hazardous in children and generally avoided in our setup, except in complex cases. We generally limit intracardiac monitoring to right atrial, LA, and PA pressure lines. Accordingly, under the definition of low output syndrome after repaired TAPVC, an integration of relevant clinical, laboratory, and bedside echocardiographic criteria was used. The criteria for diagnosis were as follows: cold extremities, absent pedal pulses, decreased toe temperature, reduced systolic pressure, impaired renal function and oliguria (<1.0 mL/[kg/hr]), metabolic acidosis, increased serum lactate levels (>2 mmol/L for >2 hours), low mixed venous oxygen saturation (<50%), and blunted sensorium in the absence of residual anastomotic or pulmonary venous obstruction.

Postoperative studies
All survivors (n=93) were examined and studied between January 2019 and December 2019, which was the closing interval of the study. Postoperative evaluation consisted of three monthly clinical examination, electrocardiogram, chest radiograph, echocardiography and computedtomographic angiography. The functional class at follow-up was noted. A minimum of 12 months follow-up was mandatory for echocardiographic and angiocardiographic evaluation. For life table analysis, complete follow-up from the day of operation till the last follow-up was taken into consideration. Computed-tomographic angiography was performed on a third generation, 384 (2 x 192) slice, dual source CT scanner and multiplanar reformatted images and volume rendered images (SOMATOM FORCE, Siemens, Germany) were reconstructed and analysed.

Techniques of Computerised-tomographic Angiography
All scans were performed on a third generation, 384 (2 x 192) slice, dual source CT scanner (SOMATOM FORCE, Siemens, Germany). It has a rotation time of up to 0.25 seconds with a temporal resolution of up to 66 milliseconds and spatial resolution of 0.24 mm.
No form of heart rate control was required. Retrospective ECG-gated CT angiography examination was performed after injection of non-ionic iodinated contrast (1.0 to 1.5 mL/Kg body weight) was administered via peripheral intravenous line at flow rates varying from 1.0 -4.5 mL/s followed by a saline chaser injected at the same flow rate. A prospectively gated flash mode acquisition at a pitch of 3.2 (table feed 737 mm s−1), and using a tube voltage of 80 kVp with automated tube current modulation was also performed. A "manual" bolus tracking method was used with acquisition manually triggered when optimal contrast opacification within the pulmonary vessels was perceived on the monitoring sequence. Automated tube voltage selection and automated tube current modulation based on body habitus (CARE kV and CARE Dose4D, Siemens Healthcare) were enabled.
Slices were reconstructed of 0.6-mm section thickness and increment of 0.4 mm, using a medium sharp kernel (Bv40), with a model based iterative reconstruction strength level 3 (ADMIRE; Siemens Healthcare). Multiplanar reformatted images and volume rendered images were reconstructed and analysed.

Operative and Postoperative Management
The surgical techniques, intra-and postoperative management protocols were uniform throughout the study period. After weaning from bypass, upon snaring the vertical vein, the MLAP increased between 18 and 21 mmHg accompanied by an acute increase in systolic PAP to greater than 50 mmHg in 82 (82.8%) patients. Loosening of the vertical vein resulted in a decrease of pressure to a ratio of 0.6 and decrease of the MLAP to 11 mmHg with a range between 10-11mmHg in all patients. This was associated with a significant increase in mean arterial blood pressure and stable hemodynamics. The PA pressure in the remaining 17 patients was between 31-50 mmHg. The vertical vein was left open on an adjustable vertical vein ligature in all patients.
The sternum was left open in 11 (11.1%) patients. Delayed sternal closure was done 24 to 48 hours after hemodynamic stability was achieved.
Median duration of inotrope requirement was 8 days (range 6-19 days). Median duration of ventilation was 4 days (range 1-20 days). Thirty-nine (39.3%) patients received total parenteral nutrition commencing after 48 hours of ventilation. After the operation, patients were weaned from digoxin, diuretics, phenoxybenzamine and angiotensin-converting enzyme-inhibitors at varying intervals.
The mean CPB time was 56.5±11.9 minutes (range 35-120 minutes), and the mean aortic cross-clamp time was 26.2±8.3 minutes (range 17-70 minutes). Left ventricular assist devices and extracorporeal membrane oxygenation (ECMO) were not used for any patient in this study group. .

Original cohort
Patients' age at operation ranged from 1 day to 25 years (mean 24.8±55.3 months, median 4 months). Thirty-five (35.2%) patients were younger than 1 month, 10 (10.1%) were between 6 and 12 months, and 24 (24.2%) were between 12 months and 25 years. Body weight ranged from 1.8 kg to 47 kg (mean 6.3±6.2 kg, median 4 kg). Most patients in this study group were small for age and 60% weighed less than 50 th percentile of predicted weight for Indian neonates and infants. Cardiac catheterization and angiocardiography were performed in the initial 19 (19.2%) patients in whom an accurate anatomical pattern was unresolved by echocardiography and in 20 (20.2%) patients to evaluate the degree of pulmonary hypertension. Since 2009, computedtomographic (CT) angiography is used as a routine to delineate the anatomic details in doubtful cases to establish the diagnosis and has almost replaced angiocardiography.
Postoperatively, all patients underwent daily echocardiographic assessment of biventricular function and flow through the vertical vein. Serial postoperative echocardiograms showed unobstructed pulmonary venous return into LA with a distended vertical vein (Figures 5A, 5B). At this point, we decided to tighten the adjustable ligature in increments. Tightening was achieved by placing additional clips outside between the polytetrafluoroethylene pledget and the previous clip, while monitoring the LA and PA pressure and arterial blood gases. Echocardiographic assessment was performed to assess the ventricular function and the degree of tightening was assessed by reduction of left-to-right shunt through the vertical vein. All ligatures were tightened gradually over a period of 24 to 96 hours, maintaining stable hemodynamics with PA pressure at subsystemic levels and normal blood gases.

Long-term outcomes
There were 2 (2.0%) late deaths 80 and 94 months after surgery due to ventricular arrhythmia (n=1) and pulmonary sepsis (n=1) respectively. Follow-up was 100% complete (1-228 months) and yielded 1695.7 patient-years of data. At a mean follow-up of 218.8 (SE± 209.9) months, the actuarial survival was 93.7 (SE±0.02%; 95% CI: 86.5, 97.1; Figure  4). All survivors were in New York Heart Association I or II at their last follow-up. There were no reoperations during this period.

Comment
The medical literature is rife with examples of therapies that seemed likely to be beneficial, but were in fact either pointless or harmful. As far as we could establish, there have been limited studies addressing the usefulness of vertical vein interruption at the time of TAPVC repair and the fate of unligated vertical vein following repair of TAPVC. There remain several unresolved queries in the medical and surgical management of TAPVC and there is no fool proof formula in the published literature to decide the optimal surgical management for a given patient.
Our 99-patient cohort is perhaps the largest so far to be evaluated for selective vertical vein patency, adjustable vertical vein ligature, LA augmentation and atrial septal fenestration on the postoperative outcomes of repaired TAPVC The age distribution was heterogenous with 35 (35.2%) patients being ≤1 month, and 24 (24.2%) were between 12 months and 25 years. The patient's socioeconomic profiles and lack of healthcare access led to their referrals for surgery at older ages. In our earlier publications, we demonstrated that late referral and late presentation lead to the development of severe PAH, malnutrition and ultimately cardiac cachexia. These factors predispose them to pulmonary infection, sepsis, postoperative pulmonary hemorrhage, and unfavourable reactions to bypass related stresses 4 . Despite aggressive perioperative management, we reported worse outcomes in whom conventional strategy of routine ligation of a discernible ascending or descending vertical vein was performed [14]. One important finding of our investigation was the occurrence of post bypass suprasystemic PA pressure due to late presentation, cardiac cachexia and cardiac pathology. Our second finding is the occurrence of suprasystemic PA pressure and unstable hemodynamics upon snaring the vertical vein after weaning from bypass. Release of the snare resulted in improved postoperative haemodynamics, early weaning from ventilatory support, and survival. In the absence of anastomotic stricture, this undesirable effect can be explained by impaired unloading of the pulmonary venous chamber due to non-compliant left-sided cardiac chambers. The benefits of an unligated vertical vein over the traditional concept of routine vertical vein ligation at the time of TAPVC repair remains debatable [16][17][18][19][20][21][22][23]. As yet, there are no universally accepted criterions for selection of patients in whom it is advantageous to maintain the patency of the vertical vein. A number of studies with small cohort of patients have been published by various investigators with conflicting results (  [13,14]. Due to nonavailability of Amplatzer vascular plug, these six patients underwent surgical ligation of the vertical vein. Although delayed closure of the vertical vein was successful in all cases it was attended by extremely high PAP in six patients and proved a difficult postoperative challenge. These findings were suggestive of a relatively small, non-compliant dysfunctional left-sided chambers or of disease-related or CPB-related pulmonary vasoreactivity [14].
There are varying reports on left heart volume characteristics in obstructive TAPVC from normal findings to the conclusion that left-sided chambers are smaller than normal and the LA lacks both normal compliance and reservoir function [5][6][7][8][9][10][11]. Such chamber abnormalities have been attributed to large left-to-right shunt causing reduced atrial filling and decreased LV relaxation secondary to elevated right ventricular diastolic pressure or volume [5][6][7][8][9][10][11]. Published literature does not address the percentage of patients with obstructive TAPVC who have structurally smaller left-sided chambers.
In order to allow the left heart to adapt and maintain adequate cardiac output, we have used an oversized Dacron patch for interatrial septation, deviating the same towards the enlarged right atrium and have incorporated part of the vertical vein to achieve structural alignment and augmentation of the LA cavity ( Figures 7A-7D) [14]. The concept of enlarging the LA is almost similar to that described by Cooley and colleagues except that in our patients, we achieved it by excising the floor of the fossa ovalis and utilizing an oversized Dacron patch graft to close the ASD and enlarge the LA [33]. Corno and colleagues described the two-patch technique of LA enlargement 34 . Subsequently, several investigators have demonstrated increased incidence of supraventricular arrhythmias due to the use of a transverse right atrial incision and division of the supraventricular crest. We have not used this technique. There is no valid argument for or against the use of Dacron patch or pericardial patch. It may be argued that fenestration in the synthetic patch may be appropriately sized at the primary operation with minimal chances of over or undersizing. There were no residual shunts through the fenestration on any surviving patient on long-term follow-up. The driving forces behind selection of patients whose vertical vein was kept patent after surgery were to reduce the PAP in the perioperative period, postoperative pulmonary hypertensive crises and mortality (Figure 2). We aimed to achieve this by leaving the vertical vein unligated so that it may function as a temporary venous reservoir for pulmonary venous blood, volume unloading the small non-compliant leftsided cardiac chambers until they are able to grow and adapt to the requisite flow demands. Secondly, the unligated vertical vein could serve as a temporary "pop-off" valve in the event of pulmonary hypertensive crises and thereafter the same may be subjected to a gradual process of occlusion 15 . Finally, despite late presentation and long standing pulmonary hypertension, we have been successful in occluding the patent vertical vein within 72 to 96 hours. (Figures 5A, 5B, 6A-6D).
The etiopathogenesis of postoperative pulmonary hypertensive crises in obstructive TAPVC may be multifactorial [25][26][27][28][29][30][31][32]. Although the pulmonary veins/capillaries do not have any valves, there are variable reactive pulmonary arteriolar changes due to the disease process and any rise in LAP will lead to pulmonary hypertensive crisis. The postoperative course targets management of these issues and occasionally ECMO is necessary while the pulmonary vascular bed recovers and LV compliance improves [14,15,24]. The time course of this adjustment is variable. It is conjectured that there is a feedback loop whereby acute elevation of LAP results in excessive pulmonary arteriolar constriction with disproportionate pulmonary hypertensive response that further exacerbates the low cardiac output state caused by a poorly compliant LV [14]. Atrial septal fenestration in patients undergoing rechanneling of TAPVC is controversial. In this study, we performed atrial septal fenestration to permit "spill over" in the setting of postoperative RV dysfunction in patients with both obstructive and non-obstructive supracardiac TAPVC with PAH. During episodes of pulmonary hypertensive crises with limited RV output, and an elevated central venous pressure (CVP), a fenestrated atrial septal patch permitted right-to-left shunting, increasing left ventricular preload and cardiac output, albeit at the expense of some degree of systemic desaturation (Figures 5A, 5B, 6A-6D). It is noteworthy that none of the survivors exhibited any shunting in the late postoperative period and none required any interventional closure of the fenestration. Thus, the unligated vertical vein, in conjunction with a calibrated atrial septal fenestration, resulted in equalization of LAP and CVP during episodes of pulmonary hypertensive crisis, and was the automatic choice to avert a dismal outcome in the perioperative period.
Our protocol is to institute mechanical circulatory assistance, if the cardiac output cannot be sustained by the currently available medical and surgical treatment. We have not used ECMO on any of our patients in this study group.
Thus, the advantages of adjustable vertical vein ligature were:  The facility to tighten the ligature under optimal physiologic conditions, concomitant with the disappearance of disease or bypassrelated pulmonary reactivity.


The ability to gradually increase the afterload to the ventricle as tolerated by the patient without causing haemodynamic instability under optimal physiologic conditions.

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The capability to occlude the vertical vein in the event of a significant left-to-right shunt and bi-ventricular failure without re-opening the sternum or employing another thoracotomy or transcatheter vein closure.
The drawback of this technique may be the inability to loosen the ligature in the event of hemodynamic decompensation. All ligatures in this cohort were tightened gradually in increments over a period of 24-96 hours maintaining stable hemodynamics.
A persistent left-to-right shunt with bi-ventricular failure through an unligated vertical vein does not necessarily relegate a patient to a secondstage operation and does not warrant modification of our selection criteria for the unligated vertical vein. They may be candidates for adjustable vertical vein ligature or transcatheter vertical vein closure .
An initial concern about the technique was the possibility of iatrogenic distortion of the left superior pulmonary vein and left brachiocephalic vein. In order to address these concerns, we have threaded the loop ligature through a polytetrafluoroethylene felt and secured it to adventitia of the vertical vein, thus preventing its displacement, and brought the arms of the silk suture through the second left intercostal space, away from the sternotomy incision, perpendicular to the vertical vein, ensuring a vertical straight lie, and avoiding distortion or occlusion of the left upper pulmonary and brachiocephalic veins ( Figures 3A-3D). Postoperatively, we performed computed-tomographic angiography on all survivors at follow-up and discovered no untoward findings (Figures 8A-8D, 9A-9C, 10A-10D).

Figure Legends of Video Presentation
A video presentation of the surgical techniques of adjustable vertical vein ligation and left atrial augmentation in obstructive supracardiac totally anomalous pulmonary venous connection (UKC's modification).
1. Following median sternotomy, the thymus was subtotally excised taking care not to expose the brachiocephalic vein. The pericardium was opened in the midline in between stay sutures using scissors and not cautery to avoid inadvertent cautery-induced ventricular fibrillation. 2. The operation was performed with moderately hypothermic cardiopulmonary bypass through angled venous cannula into the superior SVC, straight venous cannula into the IVC, and aortic cannulation. St. Thomas-II based cold hyperkalemic blood cardioplegia (1:4) and topical hypothermia were used for myocardial preservation. 3. The persistent ductus arteriosus was ligated using No.2 ductus silk suture pulling down the superior surface of the pulmonary artery at the commencement of cardiopulmonary bypass as described by Dwight McGoon. The pump flow was temporarily lowered at the time of ligation of the ductus arteriosus. 4. During the cooling phase, the vertical vein was dissected extrapericardially and looped taking care not to snare until the rechanneling was completed. Following aortic cross-clamp and administration of cardioplegia, the right pleural cavity was widely opened. The apex of the heart was lifted cephalad and to the right and dislocated into the right pleural cavity to facilitate surgical exposure. 5. A stay suture was being placed on the under surface of the left pulmonary artery and put on traction for improvement of exposure of the common pulmonary venous chamber. The tip of the left atrial appendage was tied using 2-0 silk sutures and placed on traction. 6. Long transverse incisions were made on the anterior surface of the common pulmonary venous chamber along with the length of the common pulmonary vein and the posterior surface of the left atrial appendage and body of the left atrium in between stay sutures of 6-0 polypropylene (Johnson and Johnson Ltd., Ethicon, LLC, San Lorenzo, USA). 7. The common pulmonary venous chamber and the left atrium were anastomosed using 5-0 polypropylene suture. Precautions were taken to create a large anastomoses (2.5-3.0 cm) or as large as the calculated mitral valve orifice area. 8. The heart was relocated back into the pericardial cavity. The right atrium was opened in between stay sutures 1 cm posterior and parallel to the right atrioventricular groove. The remnant of the septal tissue within the fossa ovalis was excised; the size of the atrial septal defect was measured. 9. An oversized Dacron polyester patch little larger than the size of the atrial septal defect was sutured to close the atrial septal defect in such a way that the right side of the patch was deviated to the body of the right atrium away from the margin of the atrial septal defect. This manoeuvre enhances the capacity of the left atrium. 10. A 2mm calibrated atrial septal fenestration was being performed for decompression of the right-sided chambers in the event of pulmonary hypertensive crisis. 11. The right atrium was closed in two layers using 5-0 polypropylene.
The aortic cross-clamp was being released, thus restoring blood flow to the myocardium. 12. A right angle forcep was passed around the vertical vein between its junctions with the left upper pulmonary vein and brachiocephalic vein and is looped using No.4 SUTUPAK suture (Ivory Braided Silk, SW 218, Johnson and Johnson Inc, Somerville, NJ). 13. The right angled forceps was passed again in a similar fashion, and the suture was grasped by the tip of the forceps so that the vertical vein was doubly looped. 14. Both the ends of the thread were next passed through a 0.5 cm x 0.5 cm low porosity No.1 Bard polytetrafluoroethylene pledget (Impra Inc, A Subsidiary of CR Bard, Tempe, Ariz, USA) which was anchored to the adventia of the vertical vein with interrupted 6-0 polypropylene sutures to prevent subsequent distortion or occlusion of the left upper pulmonary vein and brachiocephalic vein. 15. Both arms of the silk suture were then brought out through the second left intercostal space away from the sternotomy incision perpendicular to the vertical vein, ensuring a vertical, nonredundant straight lie without distorting the vertical vein. 16. The two ends of the sutures were next passed through a 2 x 2 cm polytetrafluoroethylene pledget and clipped together using a big ligaclip (LT 400, Ethicon, Endosurgery Inc, Cincinati, Ohio). The chest was primarily closed in layers. 17. The percutaneously adjustable device to ligate the vertical vein allows gradual tightening or loosening of the ligature under optimal physiologic conditions, without reoperating the sternum, or having to resort to another thoracotomy once the reactive components of the pulmonary hypertension disappear.

Study limitations
This study has several limitations. In as much as this is a single-surgeon, single-center study, the results may not necessarily be applicable to all centers. Given its retrospective design, there may have been unmeasured confounding factors that influence the observed outcomes. The study institution likely has a referral bias for high-risk patients with complex congenital heart diseases, prematurity, late presentation, and referral from outside the geographical area.

Conclusions
We conclude that in obstructive and non-obstructive supracardiac TAPVC, postoperative pulmonary hypertension is common, and augmenting left atrial compliance by atrioplasty is perhaps useful. By leaving a pop-off, either an atrial septal fenestration, and/or the vertical vein in the immediate postoperative period, can be helpful to maintain cardiac output when there is an expected pulmonary hypertensive crisis. One or both of these pop-offs will at some point become redundant, with shunting only left-to-right, which slowly but surely, will lead to volume overload, and they will need to be addressed. In a setting where transcatheter solutions (plugging a vertical vein or ASD fenestration with a device) are not available or prohibitively expensive, percutaneous adjustable vertical vein ligature is a useful adjunct.
It allows gradual tightening of the ligature under optimal physiologic conditions without reoperation and without causing any distortion of the left superior pulmonary vein and left brachiocephalic vein. Knowledge of this approach should contribute to the armamentarium of cardiac surgeon faced with obstructive TAPVC with pulmonary arterial hypertension.