Tissue Allergy to Bone Antigen and its Role in the Diagnosis of the Initial Degree of Generalized Periodontitis with a Different Course of the Pathological Process in the Periodontal Complex

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. already described [5, 6,7]. However, the data of its influence on vascular hemostasis and coagulation is limited.
The objective of our study was to assess the influence of a carbohydraterestricted diet up to 250 g per day for 12 weeks on collage-, adenosine 5diphosphate-(ADF), ristocetin-induced platelet aggregation (PA), the concentration of von Willebrand factor (vWF) and soluble fibrin (SF).
Material and methods. Our study included 50 AH patients with increased BMI. The approval of the local ethics committee was obtained. All patients signed written consent. They were divided into 2 groups. Patients of group 1 (n=26) were treated according to the ESC guidelines 2017 for the management of AH patients [4]. Patients of group 2 (n=24) maintained the diet with carbohydrate restriction up to 250 g per day for 12 weeks as the addition to the standard antihypertensive therapy. We used carbohydrate counting tables to control daily intake of carbohydrates. However, we recommended the compensation of daily caloric intake by increase in the consuming of proteins and fats. We excluded patients with history of heart defects, arrhythmia, heart failure stage II-III, endocrine disorders (except diabetes mellitus), malignancy, myocardial infarction and stroke, surgery or trauma within recent 6 months, chronic diseases in period of exacerbation, active infections.
We defined the nutritional state of patients according to the WHO guidelines after calculation of BMI (weight in kilograms divided in meters squared). The treatment effectiveness was controlled by BP levels. We measured BP according to the standard protocol.
We took blood in patients during the first visit and after 12 weeks of treatment. Whole blood samples were collected by phlebotomy in sodium citrate (38 g/l at a final ratio of 9:1 vol/vol) with further centrifugation. We used vWF:CBA ELISA kit to measure the binding of vWF to collagen and high molecular weight multimers of vWF according to the manufacturer's instructions (Technoclone, Austria). SF in blood plasma was quantified by double-sandwich ELISA with monoclonal antibody FnI-3C as a "catch"-site [8]. We studied PA with the Born spectrophotometric method at aggregometer "Thromlite" [9]. We used ADP disodium salt in concentration 2,0·10 6 M ("Sigma-Aldrich"/"Merck", Canada), collagen in dilution 1:2, ristocetin in concentration 0,8 mg/ml as inductors of aggregation.
We performed statistical analyses with "Stata-12". Numerical variables were presented as mean±standard deviation for normal type of distribution. Nominal variables were presented in absolute values (percentage) and compared using chisquared test. We used Student t-test for comparison of independent samples and paired t-test for paired samples. P value <0,05 was considered statistically significant.
The values of hemostasis parameters and levels of BP before and after 12 weeks of treatment are presented in Table 1. Significant lowering of BP levels was registered in both groups. We revealed significant decline in the concentrations of vWF and SF in group 2. PA-ADP after treatment in both groups changed for the . better significantly. The improvement in PA-ristocetin after course of treatment was significant only in group 2. There were no significant changes in PA-collagen after 12 weeks follow-up in both groups. Such trends may be the signs of vascular hemostasis improvement and decline in blood procoagulant activity.