Original paper
Identification and Characterization of Two Novel Esterases from a Metagenomic Library
2015 Volume 21 Issue 5 Pages 649-657
Details
2015 Volume 21 Issue 5 Pages 649-657
Esterases are biocatalysts in food industry aimed for nutrition improvements, formation of flavor, and food fermentation. Two esterases EstGX1 and EstGX2 were identified based on function-based screening of a soil metagenomic cosmid library. Enzyme properties including optimum pH, optimal temperature, tolerance to organic solvents and metal ions were measured, respectively. The activity of EstGX2 could maintain about 40% after incubated at 99°C for 55 min, and could be increased in presence of 15% ethanol. The unique properties of EstGX2, high thermostability and stability in the presence of several organic solvents, may make it a promising enzyme candidate in food industry.
