Abstract
Results of the study on the frequency of micronuclei (MN) in the meristem of Pinus pallasiana D. Don and Picea abies (L.) Karst. seedlings from natural populations and plantings of technogenically polluted territories are presented. The level of cells with MN was 0.95 ± 0.13% in the control P. pallasiana population. An increase in the level of cells with MN was observed under introduction- and technogenic-pollution conditions (2.41–3.38%). Similar results were shown for P. abies. The pathological cell contained a different amount of MN (from one to two for P. pallasiana and from one to six for P. abies). The size of MN varied from 0.13 to 14.93% of the diameter of the main nuclei in P. pallasiana cells and from 0.07 to 61.87% in P. abies cells. Multinucleated cells and cells with an irregular-shaped nucleus were observed. Correlation dependence between cytogenetic disorders and MN frequency (r = 0.98) was established. The use of MN of seedlings of studied species for the assessment of the genotoxicity of technogenically polluted areas was proposed.
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REFERENCES
Yemets, A.I., Blume, R.Ya., and Sorochinsky, B.V., Adaptation of the gymnosperms to the conditions of irradiation in the Chernobyl zone: from morphological abnormalities to the molecular genetic consequences, Cytol. Genet., 2016, vol. 50, no. 6, pp. 415–419. https://doi.org/10.3103/S0095452716060086
Kurinny, D.A. and Kostikov, I.Yu., Co-cultivation of unicellular green algae (Chlorophyta, Chlorophyceae) and lymphocytes of peripheral blood of humans as a test system for radiobiological studies, Int. J. Algae, 2017, vol. 19, no. 2, pp. 163–172. https://doi.org/10.1615/InterJAlgae.v19.i2.60
Decordier, I., Papine, A., Vande-Loock, K., Plas, G., Soussaline, F., and Kirsch-Volders, M., Automated image analysis of micronuclei by IMSTAR for biomonitoring, Mutagenesis, 2011, vol. 26, no. 1, pp. 163–168. https://doi.org/10.1093/mutage/geq063
Hussain, B., Sultana, T., Sultana, S., Al-Ghanim, K.A., Masood, Sh., Ali, M., and Mahboob, Sh., Microelectrophoretic study of environmentally induced DNA damage in fish and its use for early toxicity screening of freshwater bodies, Environ. Monit. Assess., 2017, vol. 189, pp. 115–126. https://doi.org/10.1007/s10661-017-5813-x
Rocha, C.A., Cunha, L.A., Pinheiro, R.H., Bahia, M.O., and Burbano, R.M., Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury, Genet. Mol. Biol., 2011, vol. 34, no. 4, pp. 694–697. https://doi.org/10.1590/S1415-47572011000400024
Chang, P., Li, Ya., and Li, D., Micronuclei levels in peripheral blood lymphocytes as a potential biomarker for pancreatic cancer risk, Carcinogenesis, vol. 32, no. 2, pp. 210–215. https://doi.org/10.1093/carcin/bgq247
Güez, C.M., Waczuk, E.P., Pereira, K.B., Querol, M.V., Rocha, J.B., and Oliveira, L.F., In vivo and in vitro genotoxicity studies of aqueous extract of Xanthium spinosum, Brazil. J. Pharmaceut. Sci., 2012, vol. 48, no. 3, pp. 461–467. https://doi.org/10.1590/S1984-82502012000300013
Braham, R.P., Blazer, V.S., Shaw, C.H., and Mazik, P.M., Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA, Environ. Mol. Mutagen., 2017, vol. 58, no. 8, pp. 570–81. https://doi.org/10.1002/em.22123
Kanev, M., Özdemir, K., and Gökalp, F., Genotoxic evaluation of the Ergene River, Turkey, on mosquito fish, Gambussia affinis (Baird and Girard, 1853) using the piscine micronucleus assay, Int. J. Aquat. Biol., 2016, vol. 4, pp. 330–339. https://doi.org/10.22034/ijab.v4i5.171
Kumar, M.K., Avelyno, S., and Shyama, K., Genotoxic Biomarkers As Indicators of Marine Pollution, Marine Pollution and Microbial Remediation, Singapore: Springer, 2017, pp. 263–270. https://doi.org/10.1007/978-981-10-1044-6_17
Baršienė, J., Andreikėnaitė, L., and Bjornstad, A., Induction of micronuclei and other nuclear abnormalities in blue mussels Mytilus edulis after 1-, 2-, 4- and 8-day treatment with crude oil from the North Sea, Ekologija, 2010, vol. 56, nos. 3–4, pp. 124–131. https://doi.org/10.2478/v10055-010-0018-4
Fenech, M., Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells, Mutagenesis, 2011, vol. 26, pp. 125–132. https://doi.org/10.1093/mutage/geq052
Andrade, V.M., Silva, J.D., Silva, F.R., Heuser, V.D., Dias, J.F., Yoneama, M.L., and Freitas, T.R., Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test, Environ. Mol. Mutagen., 2004, vol. 44, no. 5, pp. 459–468. https://doi.org/10.1002/em.20070
Boriollo, M.F., Resende, M.R., Silva, T.A., Publio, J.Yo., Souza, L.S., Dias, C.T., de Mello Silva Oliveira, N., and Fiorini, J.E., Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay, Genet. Mol. Biol., 2014, vol. 37, no. 2, pp. 428–438.
Saleh, K. and Sarhan, M.A., Clastogenic analysis of chicken farms using micronucleus test in peripheral blood, J. Appl. Sci. Res., 2007, vol. 3, no. 12, pp. 1646–1649.
Martínez-Haro, M., Balderas-Plata, M.A., Pereda-Solís, M.E., Arellano-Aguilar, O., Hernández-Millán, C.L., Mundo-Hernández, V., and Torres-Bugarín, O., Anthropogenic influence on blood biomarkers of stress and genotoxicity of the burrowing owl (Athene cunicularia), J. Biodivers. Endanger. Species, 2017, vol. 5, no. 3, pp. 196–199. https://doi.org/10.4172/2332-2543.1000196
Wang, Q.L., Zhang, L.T., Zou, J.H., Liu, D.H., and Yue, J.Y., Effects of cadmium on root growth, cell division and micronuclei formation in root tip cells of Allium cepa var. agrogarum L., Fyton, 2014, vol. 83, pp. 291–298.
Kalaev, V., Artyukhov, V., and Nechaeva, M., Micronucleus test of human oral buccal epithelium: problems, progress and prospects, Cytol. Genet., 2014, vol. 48, no. 6, pp. 62–80. https://doi.org/10.3103/S0095452714060061
Belousov, M.V., Mashkina, O.S., and Popov, V.N., Cytogenetic response of Scots pine (Pinus sylvestris Linnaeus, 1753) (Pinaceae) to heavy metals, Comp. Cytogenet., 2012, vol. 6, no. 1, pp. 93–106. https://doi.org/10.3897/CompCytogen.v6i1
Gökalp, F.D. and Güner, U., Induction of micronuclei and nuclear abnormalities in erythrocytes of mosquito fish following exposure to the pyrethroid insecticide lambda-cyhalothrin, Mutat. Res./Genet. Toxicol. Environ. Mutagenesis, 2011, vol. 726, pp. 104–108. https://doi.org/10.1016/j.mrgentox.2011.05.004
Zhuleva, L.Yu. and Dubinin, N.P., Use of the micronucleus test for assessing the ecological situation in regions of the Astrakhan’ district, Genetika, 1994, vol. 30, no. 7, pp. 999–1004.
Korshikov, I.I., Tkacheva, Yu.A., and Privalikhin, S.N., Cytogenetic abnormalities in Norway spruce (Picea abies (L.) Karst.) seedlings from natural populations and an introduction plantation, Cytol. Genet., 2012, vol. 46, no. 5, pp. 280–284. https://doi.org/10.3103/S0095452712050064
Goryachkina, O.V. and Sizikh, O.A., Cytogenetical reactions of conifer trees in antropogenous disturbed regions of Krasnoyarsk and its environs, Khvoinye Boreal. Zony, 2012, vol. 30, nos. 1–2, pp. 46–51.
Belousov, M.V. and Mashkina, O.S., Cytogenetic response of Scots pine (Pinus sylvestris L.) to cadmium and nickel, Tsitologiia, 2015, vol. 57, no. 6, pp. 459–464.
Dubrovna, O.V., Cytogenetic effect of NaCl and Na2SO4 on the fodder beet callus culture, Fiziol. Biokhim. Kul’t. Rast., 2005, vol. 37, no. 1, pp. 30–39.
Kunakh, V.A., Mechanisms and some regularities to somaclonal variability of the plants, Visn. Ukr. Tovar. Henet. Selekts., 2003, vol. 1, no. 1, pp. 101–106.
Bonassi, S., Coskun, E., Ceppi, M., Lando, C., Bolognesi, C., Burgaz, S., and Fenech, M., The Human MicroNucleus project on exfoliated buccal cells: The role of lifestyle, host factors, occupational exposures, health status, and assay protocol, Mutat. Res./Rev. Mutat. Res., 2011, vol. 728, pp. 88–97. https://doi.org/10.1016/j.mrrev.2011.06.005
Torres-Bugarín, O., Pacheco-Gutiérrez, A.G., Vázquez-Valls, E., Ramos-Ibarra, M.L., and Torres-Mendoza, B.M., Micronuclei and nuclear abnormalities in buccal mucosa cells in patients with anorexia and bulimia nervosa, Mutagenesis, 2014, vol. 29, no. 6, pp. 427–431. https://doi.org/10.1093/MUTAGE/GEU044
Vergolyas, M.R., Lutsenko, T.V., and Goncharuk, V.V., Cytotoxic effect of chlorophenols on cells of the root meristem of Welsh onion (Allium fistulosum L.) seeds, Cytol. Genet., 2013, vol. 47, no. 1, pp. 34–38. https://doi.org/10.3103/S009545271
Fenech, M., Kirsch-Volders, M., Natarajan, A.T., Surralles, J., Crott, J.W., Parry, J., Norppa, H., Eastmond, D.A., Tucker, J.D., and Thomas, P., Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells, Mutagenesis, 2011, vol. 26, no. 1, pp. 125–132. https://doi.org/10.1093/mutage/geq052
Luzhna, L., Kathiria, P., and Kovalchuk, O., Micronuclei in genotoxicity assessment: from genetics to epigenetics and beyond, Front. Genet., 2013, vol. 4, pp. 131–148. https://doi.org/10.3389/fgene.2013.00131
Korshikov, I.I., Lapteva, H.V., and Belonozhko, Yu.A., Pollen quality and cytogenetic changes of Scots pine as indicators of the effect of technogenic environmental pollution of Krivoy Rog, Contemp. Probl. Ecol., 2015, vol. 8, no. 2, pp. 250–255. https://doi.org/10.1134/S1995425515020109
Bajpai, A. and Singh, A.K., Meiotic Behavior of Carica papaya L.: spontaneous chromosome instability and elimination in important cvs. in North Indian conditions, Cytologia, 2006, vol. 71, no. 2, pp. 131–136. https://doi.org/10.1508/cytologia.71.131
Kalashnik, N.A., Chromosome aberrations as indicator of technogenic impact on conifer stands, Russ. J. Ecol., 2008, vol. 39, no. 4, pp. 261–271. https://doi.org/10.1134/S106741360804005X
Kovalchuk, O., Burke, P., Arkhipov, A., Kuchma, N., James, S.J., Kovalchuk, I., and Pogribny, I., Genome hypermethylation in Pinus silvestris of Chernobyla mechanism for radiation adaptation?, Mutat. Res., 2003, vol. 529, nos. 1–2, pp. 13–20. https://doi.org/10.1016/S0027-5107(03)00103-9
Korshikov, I.I., Lapteva, Ye.V., and Tkachova, Yu.A., Variation in quantitative dimensional characteristics of nucleoli and nuclei in seed cells of Pinus pallasiana D. Don (protected and human disturbed areas in the steppe zone of Ukraine), Ukr. Bot. J., 2013, vol. 70, no. 6, pp. 805–812.
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Korshikov, I.I., Belonozhko, Y.A. & Lapteva, E.V. The Use of a Micronucleus Test in Pinus pallasiana D. Don and Picea abies (L.) Karst. for the Assessment of Technogenic Pollution’s Influence. Cytol. Genet. 53, 106–112 (2019). https://doi.org/10.3103/S0095452719020051
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DOI: https://doi.org/10.3103/S0095452719020051