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Molecular-Genetic Identification of Darevskia unisexualis × D. valentini Triploid Hybrids from Sympatric Populations of Armenia

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Abstract

Seven putative triploid hybrid Darevskia lizards were genotyped on the basis of the Du215 microsatellite locus. The alleles at this locus were identified and sequenced in these individuals. We found that five out of seven individuals are triploid hybrids of Darevskia unisexualis × D. valentini, each containing two alleles of the D. unisexualis parthenospecies and one allele of D. valentini. The other two individuals were heterozygous at this locus and contained alleles only of the bisexual species D. valentini.

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ACKNOWLEDGMENTS

We are grateful to V.E. Spangenberg and E.A. Galoyan for providing the biosamples of lizards. The work assessing qualitative and quantitative parameters of DNA was performed using the equipment of the specialized complex of the Institute of Gene Biology, Russian Academy of Sciences.

Funding

This research was supported by the Russian Science Foundation, project no. 19-14-00083.

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Authors and Affiliations

  1. Institute of Gene Biology Russian Academy of Sciences, 119334, Moscow, Russia

    A. E. Girnyk, A. A. Vergun & A. P. Ryskov

  2. Moscow Pedagogical State University, 119991, Moscow, Russia

    A. A. Vergun

  3. Yerevan State University, 0025, Yerevan, Armenia

    M. S. Arakelyan

Authors
  1. A. E. Girnyk
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  2. A. A. Vergun
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  3. M. S. Arakelyan
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  4. A. P. Ryskov
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Corresponding author

Correspondence to A. E. Girnyk.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. The study used fragments of lizard tails. Work with animals was performed in compliance with the standards developed by the Ministry of Nature Protection of Armenia (permit code 5/22.1/51043). The study was approved by the Ethics Committee of Moscow State University (permit no. 24-01) and was conducted in strict accordance with ethical principles and scientific standards.

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Translated by A. Lisenkova

ADDITIONAL INFORMATION

The present work is based on the materials of the IX International School of Young Scientists in Molecular Genetics (March 2021).

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Girnyk, A.E., Vergun, A.A., Arakelyan, M.S. et al. Molecular-Genetic Identification of Darevskia unisexualis × D. valentini Triploid Hybrids from Sympatric Populations of Armenia. Mol. Genet. Microbiol. Virol. 36, 201–203 (2021). https://doi.org/10.3103/S0891416821040078

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  • DOI: https://doi.org/10.3103/S0891416821040078

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