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Cloning and Heterologous Expression of Hepatitis B Virus Pre-Surface Antigen 1

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Abstract

Hepatitis B causes major deathly infections in developing countries. The surface antigen preS1, hepatocytes attachment site, plays a crucial role in development and progression of disease. The present study was, therefore, an effort to develop an efficient expression system for PreS1 gene in Esherishia coli. PreS1 gene was amplified and cloned in pTZ57R/T vector. Following confirmation through restriction enzyme digestion and sequencing, it was subcloned in pET22b(+). E. coli BL21 (DE3) CodonPlus cells were transformed with the recombinant plasmid. PreS1 gene was induced using IPTG, the protein was expressed and quantified with Bradford assay and analyzed on SDS-PAGE. Enzyme Linked Immunosorbent Assay and Western Blot analysis were performed for protein integrity and conformation.

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Authors and Affiliations

  1. Department of Life Sciences, School of Science, University of Management and Technology, C-II Johar Town, 54770, Lahore, Pakistan

    Rana Muhammad Mateen, Muhammad Ali & Muhammad Sohail Afzal

  2. Institute of Biochemistry and Biotechnology, University of the Punjab, 54000, Lahore, Pakistan

    Asma Tariq, Zahoor Qadir Samra & Muhammad Amin Athar

Authors
  1. Rana Muhammad Mateen
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  2. Asma Tariq
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  3. Muhammad Ali
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  4. Muhammad Sohail Afzal
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  5. Zahoor Qadir Samra
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  6. Muhammad Amin Athar
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Correspondence to Rana Muhammad Mateen.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Rana Muhammad Mateen, Tariq, A., Ali, M. et al. Cloning and Heterologous Expression of Hepatitis B Virus Pre-Surface Antigen 1. Mol. Genet. Microbiol. Virol. 35, 112–116 (2020). https://doi.org/10.3103/S089141682002007X

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  • DOI: https://doi.org/10.3103/S089141682002007X

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