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Morphofunctional Characteristics of Fibroblasts of the Papillary and Reticular Layers of Human Skin Dermis

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Abstract—

Dermal fibroblasts are a heterogeneous population consisting of several subpopulations that differ in their origin, anatomical regional specificity, and location inside the dermis. Certain phenotypic and functional features of fibroblasts of the papillary and reticular layers of human skin dermis were investigated. It has been found that the introduction of reticular fibroblasts into the primary culture with an oxygen level reduced to 5% increases the efficiency of obtaining this population of fibroblasts. Morphometric analysis of cultured fibroblasts of the studied populations, comparison of the strength and rate of collagen gel contraction, and data on the expression levels of CD90 and CD73 obtained by flow cytometry has shown significant differences in fibroblasts of the papillary and reticular layers of the human dermis in the temporal region.

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Funding

The research was funded by Russian Science Foundation, project no. 21-74-30015.

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Authors and Affiliations

  1. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    O. S. Rogovaya, L. Sh. Izmailova & E. A. Vorotelyak

  2. Department of Biology, Moscow State University, 119234, Moscow, Russia

    A. O. Zupnik

Authors
  1. O. S. Rogovaya
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  2. A. O. Zupnik
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  3. L. Sh. Izmailova
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  4. E. A. Vorotelyak
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Correspondence to O. S. Rogovaya, A. O. Zupnik, L. Sh. Izmailova or E. A. Vorotelyak.

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Statement of compliance with standards of research involving humans as subjects. The research was conducted in accordance with the ethical standards of experiments involving human cell cultures and approved by the bioethical committee of Koltsov Institute of Developmental Biology.

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HUMANS AS SUBJECTS

Statement of compliance with standards of research involving humans as subjects. The research was conducted in accordance with the ethical standards of experiments involving human cell cultures and approved by the bioethical committee of Koltsov Institute of Developmental Biology.

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Translated by E. Sherstyuk

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Rogovaya, O.S., Zupnik, A.O., Izmailova, L.S. et al. Morphofunctional Characteristics of Fibroblasts of the Papillary and Reticular Layers of Human Skin Dermis. Moscow Univ. Biol.Sci. Bull. 76, 225–231 (2021). https://doi.org/10.3103/S0096392521040106

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  • DOI: https://doi.org/10.3103/S0096392521040106

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