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Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I

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Abstract

Innate immunity functions as the first line against infection, which is mediated by series of innate immune receptors. RIG-I like receptors (RLR) recognize the cytosolic nucleic acids mainly from viruses, trigger the type I interferon (IFN) production, and thus play essential roles in the anti-viral immunity. Here we cloned the mouse RIG-I (mRIG-I) cDNA into the pENTR vector using the TOPO cloning technology, and transferred the FLAG tagged mRIG-I gene from pENTR vector to destination pLenti viral vector. The expression of mRIG-I in transfected cells was detected by immunoblotting using anti-FLAG and anti-RIG-I antibodies. The transfected mRIG-I was active capable of inducing downstream IFN stimulated gene (ISG) gene transcription as well as protein expression.

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Funding

The work was partly supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, China and National Institute of Allergy and Infectious Diseases (NIH/NIAID), US (R01 AI118896).

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Authors and Affiliations

  1. College Veterinary Medicine, Yangzhou University, 225009, Yangzhou, China

    Tangjie Zhang & Jianzhong Zhu

  2. School of Medicine, University of Pittsburgh, 15232, Pittsburgh, USA

    Saumendra N. Sarkar & Jianzhong Zhu

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  1. Tangjie Zhang
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  2. Saumendra N. Sarkar
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  3. Jianzhong Zhu
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Correspondence to Tangjie Zhang.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Tangjie Zhang, Sarkar, S.N. & Zhu, J. Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I. Cytol. Genet. 53, 325–329 (2019). https://doi.org/10.3103/S0095452719040121

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  • DOI: https://doi.org/10.3103/S0095452719040121

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