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Design and Properties of Novel Proteasome Substrates Containing a Polyglutamine Sequence

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Moscow University Chemistry Bulletin Aims and scope

Abstract

Fluorogenic polyglutamine-containing peptides with five and ten glutamine residues in a row, having a FRET pair of EDANS (fluorophore) and Dabcyl (quencher), are characterized using spectral and mass spectrometric methods. The possibility of their hydrolysis by the 20S proteasome is examined. The kinetic parameters (catalytic efficiency) for these substances are determined. The presence of glycine in the substrate significantly decreases the solubility of the substrate and diminishes the efficiency of hydrolysis with the proteasome.

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References

  1. Paulson, H.L., Shakkottai, V.G., Clark, H.B., and Orr, H.T., Nat. Rev. Neurosci., 2017, vol. 18, no. 10, p.613.

    Article  CAS  PubMed  Google Scholar 

  2. Jansen, A.H.P., Reits, E.A.J., and Hol, E.M., Front. Mol. Neurosci., 2014, vol. 7, p.73.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Pratt, G. and Rechsteiner, M., J. Biol. Chem., 2008, vol. 283, no. 19, p. 12919.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Venkatraman, P., Wetzel, R., Tanaka, M., Nukina, N., and Goldberg, A.L., Mol. Cell, 2004, vol. 14, no. 1, p.95.

    Article  CAS  PubMed  Google Scholar 

  5. Jana, N.R., Zemskov, E.A., Wang, Gh., and Nukina, N., Hum. Mol. Genet., 2001, vol. 10, no. 10, p. 1049.

    Article  CAS  PubMed  Google Scholar 

  6. Juenemann, K., Schipper-Krom, S., Wiemhoefer, A., Kloss, A., Sanz Sanz, A., and Reits, E.A., J. Biol. Chem., 2013, vol. 288, no. 38, p. 27068.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Belogurov, A.A., Jr., Ponomarenko, N.A., Govorun, V.M., Gabibov, A.G., and Bacheva, A.V., Dokl. Biochem. Biophys., 2009, vol. 425, no. 1, p.68.

    Article  CAS  PubMed  Google Scholar 

  8. Bacheva, A.V., Belogurov, A.A., Ponomarenko, N.A., Knorre, V.D., Govorun, V.M., Serebryakova, M.V., and Gabibov, A.G., Acta Nat., 2009, vol. 1, no. 1, p.84.

    CAS  Google Scholar 

  9. Bacheva, A.V., Belogurov, A.A., Kuzina, E.S., Serebryakova, M.V., Ponomarenko, N.A., Knorre, V.D., Govorun, V.M., and Gabibov, A.G., Russ. J. Bioorg. Chem., 2011, vol. 37, no. 1, p.39.

    Article  CAS  Google Scholar 

  10. Belogurov, A.Jr., Kuzina, E., Kudriaeva, A., Kononikhin, A., Kovalchuk, S., Surina, Y., Smirnov, I., Lomakin, Y., Bacheva, A., Stepanov, A., Karpova, Y., Lyupina, Y., Kharybin, O., Melamed, D., Ponomarenko, N., Sharova, N., Nikolaev, E., and Gabibov, A., FASEB J., 2015, vol. 29, no. 5, p. 1901.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Kuzina, E.S., Chernolovskaya, E.L., Kudryaeva, A.A., Zenkova, M.A., Knorre, V.D., Surina, E.A., Ponomarenko, N.A., Bobik, T.V., Smirnov, I.V., Bacheva, A.V., Belogurov, A.A., Gabibov, A.G., and Vlasov, V.V., Dokl. Biochem. Biophys., 2013, vol. 453, no. 1, p.300.

    Article  CAS  PubMed  Google Scholar 

  12. Wang, G.T., Matayoshi, E., Huffaker, H.J., and Krafft, G.A., Tetrahedron Lett., 1990, vol. 31, p. 6493.

    Article  CAS  Google Scholar 

  13. Bacheva, A.V., Korobkina, O.V., Nesterova, P.S., Kryachkov, V.A., and Gabibov, A.G., Moscow Univ. Chem. Bull. (Engl. Transl.), 2016, vol. 71, no. 2, p.97.

    Article  Google Scholar 

  14. Piccinini, M., Tazartes, O., Mostert, M., Musso, A., De-Marchi, M., and Rinaudo, M.T., Mol. Brain Res., 2000, vol. 76, no. 1, p. 103.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    V. A. Kryachkov, V. N. Tashlitsky & A. V. Bacheva

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  1. V. A. Kryachkov
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  2. V. N. Tashlitsky
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  3. A. V. Bacheva
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Correspondence to A. V. Bacheva.

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Original Russian Text © V.A. Kryachkov, V.N. Tashlitsky, A.V. Bacheva, 2018, published in Vestnik Moskovskogo Universiteta, Seriya 2: Khimiya, 2018, No. 4, pp. 275–281.

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Kryachkov, V.A., Tashlitsky, V.N. & Bacheva, A.V. Design and Properties of Novel Proteasome Substrates Containing a Polyglutamine Sequence. Moscow Univ. Chem. Bull. 73, 166–172 (2018). https://doi.org/10.3103/S0027131418040053

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  • DOI: https://doi.org/10.3103/S0027131418040053

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