Abstract
The influence of the pH and temperature of the mobile phase on the retention and separation of 14 vitamins and vitameric forms has been studied for four different stationary phases. The chromatographic conditions allowing the separation of 12 water-soluble vitamins and vitameric forms have been proposed. It has been established that the best separation of water-soluble vitamins can be achieved by employing gradient elution. The mobile phase A, (0.6% phosphoric acid) pH 1.5–1.8; mobile phase B, acetonitrile. For the separation of nicotinic and ascorbic acid it is preferable to use stationary phases Luna C18(2) and Gemini C18, while for the separation of riboflavin and riboflavin-5-phosphate the Synergy Hydro RP and Zorbaz SB-C18 stationary phases should be used. The selected conditions have been used for the determination of vitamins in commercial samples of vitamin preparations. The results are in good accordance with the producer data.
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References
Eitenmiller, R.R., Ye, L., and Landen, W.O., Vitamin analysis Analysis for the Health and Food Sciences, Boca Raton, 2008.
Zempleni, J., Rucker, R.B., McCormick, D.B., and Suttie, J.W., Handbook of Vitamins, New York, 2007.
Gosudartvennaya Farmakopeya Rossiiskoi Federatsii (State Pharmacopoeia of Russian Federation), Moscow, 2008.
Marszaii, M.L., Lebiedzicska, A., Czarnowski, W., and Szefer, P., J. Chromatogr. A., 2005, vol. 1094, p. 91.
Amin, M. and Reusch, J., Analyst, 1987, vol. 112, p. 989.
Woolard, D.C., and Indyk, H.E., J. AOAC Int., 2002, vol. 85, p. 945.
Llorenc, M.P., Capella-Perity, M.E., Gil-Agusin M., and Esteve-Romero J., J. Chromatogr. A, 2003, vol. 984, p. 223.
Li, K., Biomed. Chromatogr., 2002, vol. 16, p. 504.
Amiduic, B., Brboric, J., Kudina, O., and Vladimirov, S., J. Serb. Chem. Soc., 2005, vol. 70, p. 1229.
Li Y. and Brown P.R., J. Liq. Chromatogr. Relat. Technol., 2003, vol. 26, p. 1769.
Li, H.B. and Chen, F., J. Sep. Sci., 2001, vol. 24, p. 271.
Heudi, O., Kilinc, T., and Fontannaz, P., J. Chromatogr. A, 2005, vol. 1070, p.49.
Moreno, P. and Salcado, P., J. Chromatogr. A., 2000, vol. 870, p. 207.
Klejdus, B., Petrlova, J., Potechil, D., Adam, V., Mikelova, R., Vacek, J., Kizek, R., and Kubam, V., Anal. Chim. Acta, 2004, vol. 520, p. 57.
Albala-Hurtado, S., Veciana-Nogues, M.T., Izquierdo-Pulido, N., and Marine-Font, A., J. Chromatogr. A, 1997, vol. 778, p. 247.
Maeda, Y., Yamamoto, M., Owada, K., Sato, S., Masui, T., and Nakazawa, H., J. AOAC Int., 1989, vol. 72, p. 244.
Ekinci, R., and Kadakal, B., Acta Chromatogr. aphica, 2005, vol. 15, p. 289.
Lebiedzicska, A., Marszaii, M.L., Kuta, J., and Szefer, P., J. Chromatogr. A, 2007, vol. 1173, p. 71.
Dawson, K.R., Unklesbay, N.F., and Hedrick, H.B., J. Agric. Food Chem., 1988, vol. 36, p. 1176.
Wehling, R.L., and Wetzel, D.L., J. Agric. Food. Chem., 1984, vol. 32, p. 1326.
Zafra-Gemez, A., Garballo, A., Morales, J.C., and Garcia-Ayuso, L.E., J. Agric. Food Chem., 2006, vol. 54., p. 4531.
Presoto, A.E.F., Rios, M.D.G., and De Almeida-Muradian, L.B., J. Braz. Chem. Soc., 2004, vol. 15, p. 136.
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Original Russian Text © A.A. Bendryshev, E.B. Pashkova, A.V. Pirogov, O.A. Shpigun, 2010, published in Vestnik Moskovskogo Universiteta. Khimiya, 2010, No. 4, pp. 315–324.
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Bendryshev, A.A., Pashkova, E.B., Pirogov, A.V. et al. Determination of water-soluble vitamins in vitamin premixes, bioacitve dietary supplements, and pharmaceutical preparations using high-efficiency liquid chromatography with gradient elution. Moscow Univ. Chem. Bull. 65, 260–268 (2010). https://doi.org/10.3103/S0027131410040103
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DOI: https://doi.org/10.3103/S0027131410040103