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Spectrophotometric determination of flavonoids using their diazotization with (4-nitrophenyl)diazonium tetrafluoroborate

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Abstract

(4-Nitrophenyl)diazonium tetrafluoroborate (4-NPD) has been proposed as the reagent for the spectrophotometric determination of flavonoids. 4-NPD has been demonstrated to undergo diazotization reaction with quercitin, naringenin, chrysin, morin, rutin, and naringin in an alkaline medium leading to the formation of yellow-orange products. The conditions of the spectrophotometric reaction have been optimized. A procedure for the spectrophotometric determination of flavonoids with the detection limits (1.5–3.9) × 10−7 M (0.05–0.16 μg/ml) has been developed. Quercitin has been determined in pharmaceutical preparations.

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References

  1. Belikov V.G., Farmacevticheskaya khimiya [Pharmaceutical chemistry], vol. 2, Spetsialnaya farmacevticheskaya khimiya [Special pharmaceutical chemistry], Pyatigorsk, 2003, p. 437.

    Google Scholar 

  2. Ding X.-P., Qi J., Chang Y.-X., Mu L.-L., Zhu D.-N., and Yu B.-Y., J. Chromatogr. A, 2009, vol. 1216, p. 2204.

    Article  CAS  Google Scholar 

  3. Meshbah M.K., Khalifa S.I., El-Gindy A., and Tawfik K. A., Il Farmaco, 2005, vol. 60, p. 583.

    Article  Google Scholar 

  4. Davis W.B., Anal. Chem., 1947, vol. 19, p. 476.

    Article  CAS  Google Scholar 

  5. Tarola A. M. and Giannetti V., Chromatographia, 2007, vol. 65, p. 367.

    Article  CAS  Google Scholar 

  6. Wang S.-P. and Huang K.-J., J. Chromatogr. A, 2004, vol. 1032, p. 273.

    Article  CAS  Google Scholar 

  7. Molinelli A., Weiss R., and Mizaikoff B., J. Agric. Food. Chem., 2002, vol. 50, p. 1804.

    Article  CAS  Google Scholar 

  8. Jelia P., Rouseff R., Goodner K., and Widmer W., J. Agric. Food Chem., 1998, vol. 46, p. 242.

    Article  Google Scholar 

  9. Careri M., Elviri L., Mangia A., and Musci M., J. Chromatogr. A, 2000, vol. 881, p. 449.

    Article  CAS  Google Scholar 

  10. Manach C., Regerat F., Texier P., Agullo G., Demigne C., and Remesy C., Nuts. Res., 1996, vol. 16, p. 517.

    Article  CAS  Google Scholar 

  11. Wei B.L., Lu C.M., Tsao L.T., Wang J.P., and Lin C.N., Planta Med. A, 2001, vol. 67, p. 745.

    Article  CAS  Google Scholar 

  12. Kuntic V., Pejic N., Micic S., Vukojevic V., Vujuc Z., and Malesev D., J. Serb. Chem. Soc., 2005, vol. 70, p. 753.

    Article  Google Scholar 

  13. Pejic N., Kuntic V., Vujuc Z., and Micic S., Il Farmaco, 2004, vol. 59, p. 21.

    Article  CAS  Google Scholar 

  14. Hassan H.N.A., Barsou, B.N., and Habib I.H.I., J. Pharm. Biomed. Anal., 1999, vol. 20, p. 315.

    Article  CAS  Google Scholar 

  15. Korenman I.M., Fotometricheskii analiz. Metody opredeleniya organicheskikh veschestv. (Photometrical analysis. The methods of the determination of organic compounds), Moscow, 1970.

  16. Baranowska I. and Rarog D., Talanta, 2001, vol. 55, p. 209.

    Article  CAS  Google Scholar 

  17. Dowd L.E., Anal. Chem., 1959, vol. 31, p. 1184.

    Article  CAS  Google Scholar 

  18. Kaushal G.P., Sekhon B.S., and Bhatia I.S., Mikrochim Acta [Wien], 1979, vol. 1, p. 365.

    CAS  Google Scholar 

  19. Barnum D.W., Anal. Chim. Acta., 1977, vol. 89., p. 157.

    Article  Google Scholar 

  20. Kuntic V., Blagojevic S., Malesev D., Radovic Z., and Bogavac M., Monatshefre Chemie, 1998, vol. 129, p. 41.

    CAS  Google Scholar 

  21. Zel’tser L.E., Talipov Sh.T., and Morozova L.A., Zhurn. Anal. Khim., 1981, vol. 36, p. 1477.

    Google Scholar 

  22. Lim X.-Q., He J.-B., and Zha Z.-G., Sens. Act. B, 2006, vol. 119, p.608.

    Article  Google Scholar 

  23. Xiao P., Zhou Q., Xiao F., Zhao F., and Zeng B., Int. J. Electrochem. Sci., 2006., vol. 1, p. 228.

    CAS  Google Scholar 

  24. Zhang S., Dong S., Chi L., He P., Wang Q., and Fang Y., Talanta, 2008, vol. 76, p. 780.

    Article  CAS  Google Scholar 

  25. Freitas K.H.G., Mederiros R.A., and Fatibello-Filho O, Anal. Lett., 2009, vol. 42, p. 881.

    Article  Google Scholar 

  26. Pedrosa V. A., Malagurri A.R., Mazo L.H., and Avaca L.A., Anal. Lett., 2006, vol. 39, p. 2737.

    Article  CAS  Google Scholar 

  27. Adam V., Mikelova R., Hubalek J., Hanustiak P., Beklova M., Hodek P., Horna A., Trnkova L., Stiborova M., Zeman L., and Kizek R., Sensors, 2007, vol. 7, p. 2402.

    Article  CAS  Google Scholar 

  28. Kostic D.A., Mitic S.S., and Miletic G.Z., J. Serb. Chem. Soc., 2004, vol. 69, p. 477.

    Article  CAS  Google Scholar 

  29. Xu X., Ye H., Wang W., and Chen G., J. Agric. Food. Chem., 2005, vol. 53, p. 5853.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Analytical Chemistry, Moscow State University, Moscow, Russia

    V. A. Kudrinskaya, S. G. Dmitrienko & Yu. A. Zolotov

Authors
  1. V. A. Kudrinskaya
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  2. S. G. Dmitrienko
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  3. Yu. A. Zolotov
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Correspondence to S. G. Dmitrienko.

Additional information

Original Russian Text © V.A. Kudrinskaya, S.G. Dmitrienko, Yu.A. Zolotov, 2010, published in Vestnik Moskovskogo Universiteta. Khimiya, 2010, No. 4, pp. 296–301.

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Kudrinskaya, V.A., Dmitrienko, S.G. & Zolotov, Y.A. Spectrophotometric determination of flavonoids using their diazotization with (4-nitrophenyl)diazonium tetrafluoroborate. Moscow Univ. Chem. Bull. 65, 244–248 (2010). https://doi.org/10.3103/S0027131410040073

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  • DOI: https://doi.org/10.3103/S0027131410040073

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