Based on problem definition perspectives, there have two kinds of arguments about the framing of policy issues: evolution and social construction. The evolutionists argue that, social problems are the product of social development and environment change. Therefore, the problem will be dealt with by government while it was natural matured. On the other hand, social constructionists think social problems are rarely self-evidencely problems as such. Most policy actors have to define the reality of problems according their ideas and interests and convince the government to adopt action for it. The latter are more popular in agenda setting because of most actors believe that they can create image of problem and advocacy solutions in the process of agenda setting. However, due to nonprofit organizations becoming an important player and participant in the public policy and political processes, it is valuable to analyze what they do and how they do it. There has been much theoretical discussion about nonprofit organization advocacy. But only a few empirical studies explored the process of agenda setting and the different strategies of advocacy they taken in practice. In this article, we have tried to provide a more precise portrait of the formulation of issues within nonprofit organizations and advocacy activities in which they participate. We conducted in-depth interviews with chief of executive or senior directors in organizations to obtain more detailed information on their activities regarding the processes of agenda setting and the strategies of organization's policy advocacy. Analysis of the findings indicates that there have various types of agenda setting and a lot of strategies of policy advocacy in nonprofit organizations. Although the effectiveness of strategies used to influence government and public also depended on different field. But on formulating public policy, many respondents believe that it was seemed to be the most effective to exert pressure on elected political leaders or congressmen in most cases.