Callus, mature embryos, immature embryos and fresh corns of Calocedrus formosana were used as explants. Multi-buds were induced from callus cultured in WPM medium supplemented with 0.05mg/L NAA and 1mg/L BA. Somatic embryos were induced in WPM medium supplemented with 0.05mg/L 2,4-D and 2mg/L BA. Callus was easier to induce organogenesis while cultured in WPM medium supplemented with 2,4-D than NAA. The higher concentration of 2,4-D with lower concentration of BA can induce soft and white callus from callus has browning. In the surface sterilization, immature embryo were decontaminated completely, the mature embryo removes the endosperm were obtained the lower pollution rate. After 8 weeks, culture the vigor of fresh corns dissolved gradually. There was no response in the embryo cultured in WPM medium supplemented with coconut milk.