With more nutrient contents than honey, royal jelly is regarded as an especially healthy food. Separation of royal jelly from larvae is a difficult, labor intensive process. However, production of royal jelly in Taiwan still relies completely on time-consuming, manual production. The objective of this study is to develop a machine for harvesting royal jelly. During the last year, a prototype 'centrifugator' powered by an alternative motor (1/2 PS and 110V) was manufactured and tested for effectiveness in separating royal jelly. The best results were obtained with the centrifugator operating at 1750 rpm and equipped with a larva-separating screen having a mesh size of 0.608 mm. In related experiments, the percentage of royal jelly residue on screen, royal jelly residue in holders, and damaged larvae are 0.73%, 1.59% and 1.45%, respectively. The harvesting operation of royal jelly by using this centrifugator takes 9.42 minutes for one run (51 holder strips) neglecting time required for the last process of scraping-off the harvested royal jelly. When compared with the operating time of the manual harvesting operation (74.8 minutes) for the same workload, the developed centrifugator reduced the required labor tremendously.