One hundred ninety hexanic and ethanolic extracts from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 μg/ml. Fourteen extracts from 7 species showed activity (>65% mortality) against the larvae. Of these, Duguetia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 μg/ml, respectively. Annona crassiflora and Cybistax antisyphilitica showed activity at 23.06 and 27.61 μg/ml. The larvicidal properties of these species are described for the first time, and may prove to be promising in active chemical compound isolation.