The infrared microspectroscopy beamline at the National Synchrotron Radiation Research Center (NSRRC) has been adapted to provide intense collimated radiation for a mid-infrared microscope. Infrared microscope with high brightness synchrotron radiation has greatly improved the spatial resolution of this technique by allowing high quality spectra to be collected from samples whose size is approaching the diffraction limit for this wavelength of light, typical 8-10μm. In this study, the infrared spectral images of colorectal tissue showed that the absorbance of amide bands for normal tissues was much lower than that of the malignant tissue. The decrease in absorbance of phosphate of DNA was found in malignant tissue.