Anthers at uninucleate stage of two cultivars, Tainan No. 11 (TN11) and Tainong No. 5 (TN5) cultured on MS basal medium containing 2 mg/l 2, 4-D and 0.5 mg/l kinetin with addition of 30, 60, or 90 g/l sucrose , respectively.All the three media gave successful induction of callia, and showed that, when the level of sucrose increased, the callus induction rate decreased (92.2%, 92.1% and 83.2%) in TN11, while the rate increased (76.7%, 89.3% and 94.6%) in TN5. Both TN11 and TN5 had the heightest growth index of callus in D60 medium. After thirty days, both TN11 and TN5 cultured on D60 medium obtained the 2.0% of somatic embryo induction rate. When anthers of TN11 on D60 and D90 media, or TN5 on D30, D60 or D90 media, there produced the white protrusions. Observation by parraffin section showed that the somatic embryo stage had developed to torpedo stage and the white protrusions were the differentiated roots with vascular system inside.