Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer

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Tanya Johnson^1,2,7, Allal Ouhtit^3,7, Rajiv Gaur^3,7, Augusta Fernando^3,7, Paul Schwarzenberger^4,7, Joseph Su^5,7, Mohamed F Ismail⁸, Hassan I El-Sayyad⁸, Anjali Karande⁶, Zakaria Abd Elmageed^3,7, Prakash Rao⁷, Madhwa Raj^1,2,7,*

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¹ 1 Department of Biochemistry, Louisiana State University Health Sciences Center, New Orleans, LA

² Department of ObstetricsGynecology, Louisiana State University Health Sciences Center, New Orleans, LA

³ Department of Pathology, Louisiana State University Health Sciences Center, New Orleans, LA

⁴ Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA

⁵ School of Public Health, Indian Institute of Science, Bangalore, India

⁶ Department of Biochemistry, Indian Institute of Science, Bangalore, India

⁷ Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA

⁸ Department of Zoology, Faculty of Science, Mansoura University, Egypt

Front. Biosci. (Landmark Ed) 2009, 14(10), 3634–3640; https://doi.org/10.2741/3477

Published: 1 January 2009

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Abstract

Riboflavin carrier protein (RCP) is a growth- and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was over-expressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.

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