Research Article


DOI :10.26650/IstanbulJPharm.2019.418892   IUP :10.26650/IstanbulJPharm.2019.418892    Full Text (PDF)

Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line

Alim Hüseyin DokumacıPeter Olutope FayemiMükerrem Betül Yerer Aycan

Callistemon citrinus is a member of Myrtaceae family that thrives under different ecological conditions. The leaves, flowers, stem backs and roots of the plant contain various phytochemicals that are useful in folk medicine for different remedies such as antimicrobial, anti-nociceptive, fungicide and anti-inflammatory purposes. In this study, we investigated the cytotoxic effect of Callistemon citrinus leaf and flower methanolic extracts against human Colo-205 Cell Line using real time cell analyzer device for monitoring in time-dependent manner. To determine the mechanism of cytotoxicity of the extracts, Western blotting assay was used for measuring evocation of Akt pathway. Extracts were found to exert cytotoxic effect at a dose dependent manner. IC50 values of leaves and flowers extract were 6.49 µg/mL and 5.22 µg/mL, respectively. At the early stages of the experiment, Akt pathway was triggered at high extract concentrations. Although, high extract concentrations showed proliferative effect at early stages, this effect reversed after 5 and 8 h resulting in low cell viability. Findings from this study therefore showed that extracts of leaf and flower from Callistemon citrinus demonstrated cytotoxic effect against Colo-205 but seems not to be related Akt signaling pathway.


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APA

Dokumacı, A., Fayemi, P., & Yerer Aycan, M. (2019). Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. İstanbul Journal of Pharmacy, 49(1), 25-32. https://doi.org/10.26650/IstanbulJPharm.2019.418892


AMA

Dokumacı A, Fayemi P, Yerer Aycan M. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. İstanbul Journal of Pharmacy. 2019;49(1):25-32. https://doi.org/10.26650/IstanbulJPharm.2019.418892


ABNT

Dokumacı, A.; Fayemi, P.; Yerer Aycan, M. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 1, p. 25-32, 2019.


Chicago: Author-Date Style

Dokumacı, Alim Hüseyin, and Peter Olutope Fayemi and Mükerrem Betül Yerer Aycan. 2019. “Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line.” İstanbul Journal of Pharmacy 49, no. 1: 25-32. https://doi.org/10.26650/IstanbulJPharm.2019.418892


Chicago: Humanities Style

Dokumacı, Alim Hüseyin, and Peter Olutope Fayemi and Mükerrem Betül Yerer Aycan. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line.” İstanbul Journal of Pharmacy 49, no. 1 (Apr. 2024): 25-32. https://doi.org/10.26650/IstanbulJPharm.2019.418892


Harvard: Australian Style

Dokumacı, A & Fayemi, P & Yerer Aycan, M 2019, 'Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line', İstanbul Journal of Pharmacy, vol. 49, no. 1, pp. 25-32, viewed 27 Apr. 2024, https://doi.org/10.26650/IstanbulJPharm.2019.418892


Harvard: Author-Date Style

Dokumacı, A. and Fayemi, P. and Yerer Aycan, M. (2019) ‘Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line’, İstanbul Journal of Pharmacy, 49(1), pp. 25-32. https://doi.org/10.26650/IstanbulJPharm.2019.418892 (27 Apr. 2024).


MLA

Dokumacı, Alim Hüseyin, and Peter Olutope Fayemi and Mükerrem Betül Yerer Aycan. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line.” İstanbul Journal of Pharmacy, vol. 49, no. 1, 2019, pp. 25-32. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.418892


Vancouver

Dokumacı A, Fayemi P, Yerer Aycan M. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. İstanbul Journal of Pharmacy [Internet]. 27 Apr. 2024 [cited 27 Apr. 2024];49(1):25-32. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.418892 doi: 10.26650/IstanbulJPharm.2019.418892


ISNAD

Dokumacı, Alim Hüseyin - Fayemi, Peter Olutope - Yerer Aycan, Mükerrem Betül. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line”. İstanbul Journal of Pharmacy 49/1 (Apr. 2024): 25-32. https://doi.org/10.26650/IstanbulJPharm.2019.418892



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Accepted04.10.2018

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