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Food Science and Human Wellness 2024, 13(1): 299-312 https://doi.org/10.26599/FSHW.2022.9250025
Research Article | Open Access | Issue | Published: 01 June 2023

Mogroside IIE,an in vivo metabolite of sweet agent,alleviates acute lung injury via Pla2g2a-EGFR inhibition

Show Author's Information Weichao Lüa,  Guoqing Rena,  Kuniyoshi Shimizub,c,  Renshi Lia,b( ),  Chaofeng Zhanga,b,( )
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy,China Pharmaceutical University, Nanjing 211198, China
Sino-Jan Joint Lab of Natural Health Products Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan

Peer review under responsibility of Tsinghua University Press.

Keywords:
Acute lung injury, Mogroside IIE, Secreted phospholipase A2 type IIA (Pla2g2a), Epidermal growth factor receptor (EGFR)
Cite this article:
Lü W, Ren G, Shimizu K, et al. Mogroside IIE,an in vivo metabolite of sweet agent,alleviates acute lung injury via Pla2g2a-EGFR inhibition. Food Science and Human Wellness, 2024, 13(1): 299-312. https://doi.org/10.26599/FSHW.2022.9250025
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Abstract Full text Electronic supplementary material About this article

In the face of increasingly serious environmental pollution, the health of human lung tissues is also facing serious threats. Mogroside IIE (M2E) is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori. The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury (ALI). A lipopolysaccharide (LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting, co-immunoprecipitation, and quantitative real time-PCR analysis. The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA (Pla2g2a)-epidermal growth factor receptor (EGFR). The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation. In addition, M2E protected ALI induced with LPS against inflammatory and damage which w ere significantly dependent upon the downregulation of AKT and mTOR via the inhibition of Pla2g2a-EGFR. Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury, which may represent a promising strategy to treat ALI.


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About this article

Mogroside IIE,an in vivo metabolite of sweet agent,alleviates acute lung injury via Pla2g2a-EGFR inhibition

Show Author's information Weichao Lüa, Guoqing Rena, Kuniyoshi Shimizub,c, Renshi Lia,b( ), Chaofeng Zhanga,b,( )
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy,China Pharmaceutical University, Nanjing 211198, China
Sino-Jan Joint Lab of Natural Health Products Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan

Peer review under responsibility of Tsinghua University Press.

Highlights

● Mogroside ⅡE was characterized by the highest anti-lung injury ability.

● Mogroside ⅡE is a natural Pla2g2a inhibitor among the mogrosides.

● Inhibiting the interaction of Pla2g2a and EGFR significantly relieves lung injury.

● Mogroside ⅡE inhibits the activation of EGFR-AKT-mTOR by inhibiting Pla2g2a.

Abstract

In the face of increasingly serious environmental pollution, the health of human lung tissues is also facing serious threats. Mogroside IIE (M2E) is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori. The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury (ALI). A lipopolysaccharide (LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting, co-immunoprecipitation, and quantitative real time-PCR analysis. The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA (Pla2g2a)-epidermal growth factor receptor (EGFR). The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation. In addition, M2E protected ALI induced with LPS against inflammatory and damage which w ere significantly dependent upon the downregulation of AKT and mTOR via the inhibition of Pla2g2a-EGFR. Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury, which may represent a promising strategy to treat ALI.

Keywords: Acute lung injury, Mogroside IIE, Secreted phospholipase A2 type IIA (Pla2g2a), Epidermal growth factor receptor (EGFR)

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Publication history

Received: 28 February 2022
Revised: 23 April 2022
Accepted: 08 May 2022
Published: 01 June 2023
Issue date: January 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

Acknowledgements

We would like to thank the National Natural Science Foundation (81773982; 82003937), and Youth Academic leaders of the Qinglan Project in Jiangsu province for financial support. We would like to express our gratitude to EditSprings (https://www.editsprings.com/) for the expert linguistic services provided.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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