Kigelia africana (Lam.) Benth: Sausage of Medicinal Properties

Nature had been serving humanity from ages by providing with the food and resources of our well-being. It was serving with medicines and herbs that are used to heal many disorders.  There were numerous lead molecules that had been isolated from natural sources, especially plants. Kigelia africana (Lam.) Benth is a widely distributed plant in Africa.  Savage tree or the cucumber plant is the common name of the plant. It belongs to the family Bignoniaceae. The plant is a native plant of Africa which is confined to the south, central and West African regions. Preliminary phytochemical screening was performed for the plant revealed the presence of alkaloids, tannins, saponins, flavonoids, carbohydrates and sapogenetic glycosides. The plant has been reported to contain flavonoids, aldehyde iridioids, coumarins like dihydroisocoumarin, naphthoquinones like pinnatal, isopnnatal, kigelinole and isokigelinole. It had been investigated and proven to possess various chemicals that treat inflammations, cancers, psoriasis, dysentery and bacterial infections.


INTRODUCTION
Nature had been serving humanity from ages by providing with the food and resources of our well being. It was serving with medicines and herbs that are used to heal many disorders. There were numerous lead molecules that had been isolated from natural sources, especially plants (Cordell, 2000). In this paper, one of such most noted plants of Africa is being reviewed for its traditional claims, chemical constituents that had been isolated till date and the pharmacological activities that had been performed on the plant till now.
Kigelia africana (Lam.) Benth is a widely distributed plant in Africa. The plant is a native plant of Africa which is con ined to the south, central and West African regions. Savage tree or the cucumber plant is the common name of the plant. It belongs to the family Bignoniaceae. Its fruits are huge and grow to about 60cm long and weigh about 4-5 kg. In Hindi, it is called a balm kheera plant and is wide distributed in India and most commonly in West Bengal. The plant grows about 10m high and prefers a moist climate to grow (Sofowora, 1980) (Figure 1).

Traditional claims
Traditionally this plant has claimed that the application of crushed or pounded plant topically cures abscess and wounds. The bark of the plant is used in the treatment of many venereal diseases and dysentery (Agyare et al., 2013b). The plant is claimed to treat diseases due to tapeworm, malaria and postpartum hemorrhage (Zofou et al., 2013).
Especially, roots and bark of the plant are isolated for Lapachol, ferulic acid, sterols like stigmasterols, methoxymellein and other two phenols. Kigelin is the major active principle that is isolated from the plant along with methoxymellein (Agyare et al., 2013a). Preliminary phytochemical screening was performed for the plant revealed the presence of alkaloids, tannins, saponins, lavoniods, carbohydrates and sapogenetic glycosides (Agyare et al., 2013b).
A compound named Kojic acid has been isolated from the plant and was con irmed by XRD studies. This was believed to be the metabolite of the shikimic acid pathway (Eyong et al., 2013). Three fatty acid derivatives had been isolated from the ethyl acetate extract of the fruits of K. Africana. Fractionation was done using bioassay guidance, and palmitic acid was extracted from the fruits of the plant (Grace et al., 2002).
Natural ibres are extracted from the plant using sodium hydroxide. They are bleached and characterized. The produced ibres are rough and soft. The thermal stability and crystallinity were established. They contained a majority of hemicellulose content in the ibres. The ibres also exhibited antibacterial properties and hydrophobic nature. All the other characters are similar to that of cotton and many conventional ibres (Ilangovan et al., 2020).
The plant was isolated for caffeic acid glycosides, specioside and verbascoside, including minescoside (Costa et al., 2017). Verminoside and methyl esters of caffeic acid were also isolated from Africana (Picerno et al., 2005). The plant also contains sterols like norviburtinal and isopinnatal, which is evident from a study (Jackson et al., 2000). Apart from caffeic acid, it also was elucidated for chlorogenic acid and furanone derivatives like ajugol (Gouda et al., 2003). Compounds like iridiods, phenylpropanoid derivative and eucommiol derivatives were isolated from the plant (Khan et al., 2017).

Antimalarial activity
The plant has been investigated for anti-malarial activity against the chloroquine-sensitive strain of the plasmodium and reported that the methylene chloride leaf extract of the plant showed better activity against the organism. The stem bark also was investigated for the anti-malarial activity and showed 3 activity compounds, Atranorin, Specicoside and hydroxyl cinnamic acid which are potent against Plasmodium falciparum (Zofou et al., 2011(Zofou et al., , 2013. The synergistic effect was investigated by combining the compound, Atronorin with Withaquinine and Artemether, which were potent anti-malarial drugs. Contrarily, there was an antagonistic effect of the above compounds when used in combination with P-hydroxy cinnamic acid and Specicoside (Zofou et al., 2013).

Anti-Bacterial activity
Kigelia africana, along with Strophanthus hispidus, were investigated for the antimicrobial activity. The stem bark and leaves of K. Africana was extracted with methanol and root and leaves of S. Hispidus were extracted with Methanol. These extracts were combined and tested for the antibacterial property against two gram-positive and negative bacteria. Disc diffusion method and Micro dilution methods were used to investigate for the activity. The extracts of K.africana showed a better activity with MIC higher than that of S.hispidus (Agyare et al., 2013b).
The antibacterial activity of the stem bark and fruits was tested by extracting with water, ethanol or ethyl acetate in another study. This resulted in proving the potent antibacterial activity against grampositive and negative bacteria. The method used was microtitre plate bioassay. The fruit extracts and stem bark showed similar activity in both types of bacteria (Grace et al., 2002) The antibacterial activity of the stem bark of the plant was investigated in a study conducted by Omonkhelin. They tested the activity using Staphylococccus aureus and the aqueous extract showed no activity, but there was s signi icant activity with the ethanol extract. The zone of inhibition was found to be 15.05. The extract was compared with the standard drugs like cipro loxacin, Ceftriaxime and ampicillin, including gentamicin and it produced a similar activity compared to the standard drug, amoxicillin (Owolabi et al., 2007).

Anti leishmanial activity
The plant was extracted with various solvents and tested for the antimicrobial properties on various organisms. The hexane derivatives were effective against Cryptococcus neoformans Pinh. Chloroform derivatives were active against Leishmania donovani which was elucidated for Verminoside and showed a slight inhibition of CB1, CB2. Both the extracts were active against Trypanosoma brucei. The ethyl acetate fraction of the extracts showed similar activity against all the organisms (Khan et al., 2017).

Antioxidant activity
The antioxidant activity was investigated using the DPPH free radical scavenging method. The stem bark and leaves were tested for the activity. This was compared with the root and leaves of the plant S. Hispidus. The IC 50 of stem bark and leaves extract of K. africana were determined as 56.9 and 13.7 mcg/mL, respectively and root and leaf extracts of S. hispidus were determined 49.8 and 45.1 mcg/mL, respectively (Agyare et al., 2013b).

Wound healing activity
K. africana stem bark and leaf extracts were elucidated at 7.5% w/w and were investigated for the wound healing activity and the activity was very rapid. The complete closure was seen in 9 days (Agyare et al., 2013b).

Antifungal activity
The antifungal activity of the stem bark of the plant was investigated in a study conducted by Omonkhelin. They tested the activity using candida albicans and the aqueous extract showed no activity but there was s signi icant activity with the ethanol extract. The zone of inhibition was found to be 20.75. The extract was compared with the standard drugs, including gentamicin and it produced a similar activity compared to the standard drug, amoxicillin (Owolabi et al., 2007).

Quorum Sensing (QS) inhibition
The antivirulence properties of the plant were investigated using Quorum Sensing (QS) inhibition. Four extracts, Dichloromethane, Ethyl acetate, Hexane and methanol were tested for the activity against biosensor systems of Agrobacterium tumefaciens and Chromobacterium violaceum. The inhibition of the Quorum sensing controlled production of violacein was assayed and measured using the agar diffusion assay method. The order of the activity is as follows in ascending order: methanol<ethyl acetate<dichloromethane<hexane. Both the LuxI and Lux R were investigated, which targeted the QS signal and QS receptor (Chenia, 2013).

Antiprotozoal activity
The antiprotozoal activity of the fruits of the plant was investigated using the modi ied disc diffusion method and MIC method. The activity was tested against Giardia duodenalis. Methanol, water and ethyl acetate were used as solvent of extraction. The methanol and water extracts showed potent activity against the organisms. The methanol, water, and ethyl acetate extracts also inhibited between 55 and 70 % of the growth of the gastrointestinal parasite Giardia duodenalis (Arkhipov et al., 2014).

Anticancer activity
The anti-cancer activity of the fruits of the plant was tested against the HeLa cell lines and CacO2 induced cancers in the colorimetric proliferation of cells. The proliferation of the cell lines was inhibited in the CaCO2 model by 50% and the HeLa cell lines were inhibited from the growth in the cervical cell growth using the methanol extract. Other extracts, chloro-form and hexane stimulated HeLa cell growth and were found to show proliferative effects (Arkhipov et al., 2014).

Polycystic Ovary Syndrome (PCOS)
The fruit powder of the plant was used for the treatment of PCOS in patients at a dose of one ounce twice daily. The patients were tested for Amenorrhoea and hirsutism. The herbal preparation of the fruits was used to restore the menstrual low in both the patients leading to the reduction in the acne and there was no signi icant reduction in the acnes. Overall the fruits of the plant were proven bene icial in treating the PCOS (Oyelami et al., 2012)

CNS stimulant
The stem bark of the plant was extracted with ethanol and was tested for the stimulation activity of the central nervous system. It was tested in the mice induced with sleep with the help of barbiturates in the rotarod method. The extract was able to successfully recover the mice faster from the barbiturate when compared with the standard (Owolabi et al., 2008).

CONCLUSIONS
Plants are the sources of many drugs and the sole nature of the plants of lack of side effects is the advantage of using herbs as medicines. They are the sources of numerous lead molecules that treat many dreadful diseases. K. africana is one of those plants that grow in Africa with a potency of generating various leads and competing with synthetic drugs. It had been investigated and proven to possess various chemicals that treat in lammations, cancers, psoriasis, dysentery and bacterial infections. This demands further in-depth research to isolate those leads that are used to treat the above-stated diseases and establish a prominent mechanism of action.