A Fuzzy clustering Approach for Micro Aneurysm Lesion Detection in Diabetic Retinopathy

Diabetic retinopathy (DR) be the signi(cid:977)icant dif(cid:977)iculty of diabetes, and micro aneurysm (MA) is an earliest diabetic retinopathy lesion, making early detection of MA a key factor in diabetic retinopathy. DR is a direct or indirect effect on human vision caused by chronic diabetes. During its early stages DR is asymptomatic, and the late diagnosis leads to undeviating vision loss. The computer-assisted diagnosis helps with prompt and effective care, with the aid of medical photos. MA mark the beginning of DR making it a vital screening stage for this disorder. Diabetic retinopathy is a persistent infection of eye that can be the reason of blindness unless it is diagnosed and treated in due course. Early discovery with analysis of diabetic retinopathy is vital to vision preservation of patient. Precise recognition of MA be the crucial method towards early diagnosis of DR, since they occur as the (cid:977)irst symptom of the disease. The segmentation of MA is performed using the Fuzzy C algorithm, and the extraction of features is performed with Gray Level Co-occurrence Matrix ( GLCM) as the set of characteristic for KNN. This technique aims to improve classi(cid:977)ication accuracy within an ensemble. A procedure is suggested here that recognizes the (cid:977)irst DR sign called MA using images from the retinal fundus. Effective diagnosis of DR is very critical in the defense of patients’ right to see. The procedure proposed is tested using publicly available databases of retinal images and greater accuracy is achieved.


INTRODUCTION
DR is a chronic disease that based on the number and types of lesions on the retina. Therefore, these lesions need either to monitor DR or to assess its progression. MA is the most extreme and often the irst lesions to develop as a result of DR. Diabetic retinopathy is divided in 4 phases: severe non-proliferational retinopathy in the irst step. Secondly, moderate retinopathy with no proliferation. Firstly, there is severe non-proliferative retinopathy, with proliferative retinopathy in the inal stage (Yau et al., 2012). The irst of four stages of DR is non-proliferative diabetic retinopathy (NPDR) as the smallest regions of the retinal blood low have ballon-like in lammation. Blindness will be treated if DR progresses at this point. Yet blood vessels that feed the eye are blocked by progression of the disease, leading to blindness. Any blood low is then blocked, leaving the retina inadequate for blood (Frank, 2015). Proliferative Diabetic Retinopathy (PDR) is a retinal blood vessel damaging condition. This permits the emission of vascular endothelial growth factor (VEGF). The VEGF is a material that induces abnormal formation of blood vessels on the surface of the retina. These vessels be able to quick bleed and retinal friction as well as separation. Such anomalous blood vessels occasionally turn into a danger even if the individual does not know there is a problem. Such approaches to early warning signs are discussed by different diagnose techniques (Venkatesh et al., 2015). Early treatment helps to treat illnesses. In, Figure 1 the different retinal characteristics of macula, optic nerves, blood vessels, and glass are shown. Formulated neural network model distinguish the found igure as a secure image along with a DR image (Osareh et al., 2002). The DR identi ication proposal incorporates the mutual advantages of the DR morphology classi ication method (blood vessel segmentation), Watershed Transformation (Optic Disk segmentation), in addition to GLCM. Diagnosis of retinopathy requires effective microaneurysm identi ication (Benzamin and Chakraborty, 2018). Typically, the basic approach to automatic detection of microaneurysms has four stages, 1) image pre-processing 2) candidate extraction 3) feature extraction and

4) classi ication
According to the various MA shapes and colors, the most important steps are candidate extraction and feature extraction. The total expected research contribution is given below, 1. Enhanced blood vessel identi ication through morphological iterative method.
2. To create a a fuzzy C-means clustering algorithm with pre-processed image.
3. Application of GLCM with KNN classi ier for mass retinopathy screening.
Due to DR, several regions on the retina are affected, causing vision loss. Additional characteristics such as hemorrhages, microaneurysms and exudates arise in eyes due to retinal changes (Sheeba and Vasanthi, 2011). Microaneurysm identi ication is one of DR's most important steps. Microaneurysm refers to the irst clear signs of the presence of DR. If abnormal new blood vessels (neovascularization) form as part of Proliferative Diabetic Retinopathy (PDR) at the back of the eye, they may burst and bleed as well as blur. Diabetic people can develop MA as an early symptom (Agustina, 2005;Chun-Lin, 2010). Automatic detection of DR lesions such as MA may lead to early diagnosis and screening in digital fundus images (Dehghani et al., 2012;Aundal and Aasted, 1996). This paper proposes an automatic, accurate approach to classify MA.
MAs are small swellings on the surface of tiny blood vessels, as seen in Figure 2. Such tiny swellings split, causing blood to leak into surrounding tissue. In human retina color fundus photographs, MA appear as small, circular red dot from capillaries (Kociolek et al., 2001). MA occur as remote points, i.e. detached as vascular tree, since capillaries are not visible in images of color fundus. Their diameter is usually 10 to 100 micrometers, but is often less than 125 micrometers, minor than vein width (Huang et al., 2004). These tiny aneurysms cause bleeding and blood rupture. From the viewpoint of image processing, automated recognition of MA is a daunting assignment because of similar color and scale as vessels, but with variable dimension and are frequently so small that they can easily be confused with image noise (Liskowski and Krawiec, 2016). Either a red lesion is MA or a tiny dot hemorrhage is hard to tell. In angiography, luorescence is the only way to be sure. Invasive treatment involves the patient being injected with a contrast agent. This article looks on segmenting preprocessed image MA with FCM and extracting features using wavelet, segmented image GLCM Cunefare et al., 2017).
Present DIP-induced diabetic retinopathy diagnostic approaches have certain drawbacks. Most red lesions is similar to blood vessels, causing distinction complicated. MAs typically have very small size and low background variability, rendering MA identi ication a dif icult activity. The main barrier in retina to red lesion identi ication is MA segmentation in the low-contrast zone and white lesions. When one has cataract, macular degeneration, glaucoma, or further eye disease, it is hard to spot diabetic retinopathy.

EXPERIMENTAL METHODOLOGY
Health contribution data are split into two categories of medical treatment: data where illness is present and data where infection is not diagnosed. Sensitivity and consistency tests are used to determine the quality of medication. Effective DR diagnosis is crucial to preserving patient vision skills. The proposed retinopathy discovery model includes mainly three process, preprocessing, feature extraction, and classi ication.
Preprocessing is central to image processing recognition techniques include low contrast due to eye background morphology, non-illumination, opaque media within the eye, camera wide angle optics, pupil size dissimilarity, sensor array structure and eye movement when image capture. Preprocessing thus plays a signi icant function image analysis of funds. Preprocessing technique major purpose is to enhance the possibility of illness detection through visual evaluation along with computer-aided retinal image segmentation. Figure 3 demonstrates the proposed process structure. Fuzzy C is a clustering algorithm for retinal image input from segment MAs and subsequently, texture analysis is performed and the characteristics are given toward KNN, to categorize the images as regular or MA-based.

RESULTS AND DISCUSSION
DIARETDB1 (DIAbetic RETinopathy Database Calibration Level 1) and ROC (Retinal Online Challenge) are two publicly available retinal image databases. DIARETDB1 consists of 89 color fundus images, 84 of which contain moderate non-proliferative signs of diabetic retinopathy, and 5 contain no DR signs. Image was taken using a 50-degree Field-Of camera with different image settings. These refer to a logical realistic situation where images are similar and the overall ef iciency of diagnostic methods can be calculated. Image pre-processing is a prerequisite for detecting fundus image-related anomalies to improve MA visibility in the fundus image.
Variation in color and brightness of fundus photographs attributes with photography settings. Firstly, gamma correction had done to all image for these variations, given in Figure 4. As the contrast in MAs / haemorrhages / blood vessels and context as in Figure 5 is enhanced, the gamma-corrected image removes the green channel. The suggested solution further improves the contrast between green channels by transforming values using Adaptive Histogram Equalizing (AHE). AHE is a method for enhancing image spatial contrast. The adaptive approach computes histograms that are identical to ordinary histogram equalization for distinct image sections and reallocate image buoyancy values. Therefore, it is important to enhance an image 's local contrast and provide more details.
Clustering deals with the process of arranging the collection of objects in a way that similar cluster objects be closer to one another than objects in other clusters. Clusters seeking are the best centers in iterative method (van Grinsven et al., 2016). Fuzzy clustering is a muddy logic technique that enables clusters to lexibly distribute data points (Li et al., 2018). The Universe entity that it Fuzzy set by membership range ranging real values 0 to 1, depending on Fuzzy Algebra. Data points have granted limited membership by fuzzy clustering into several neighboring clusters. Membership rates re lect the degree to which data point and cluster interact. Using membership format, different clusters are allocated data points. And the key point of fuzzy clustering is not uniformly partitioning data points within a cluster collection (Kozak et al., 2014). Membership rates are accomplished by using distance metrics calculating the distance between a point and a cluster center.
Fuzzy C method is the major fuzzy clustering algorithm used by Bezdek to adjust a crisp clustering approach. Here, Fuzzy C algorithm is applied to remove candidates as a result of deleting regions without MA-like characteristics (Ginneken, 2017). The Fuzzy C-Means algorithm is typically a straightforward implementation of the K-Means clustering algorithm's fuzzy logic. As shown in Figure 6, MAs are segmented with the blood vessels by applying Fuzzy C on the preprocessed picture means clustering algorithm. Additional blood vessels and other larger sections are removed to isolate MAs (Abràmoff et al., 2016). Input data is converted into function extraction features package. When the same functionality is removed, as an alternative of full input size, the feature set is required to take out pertinent information and execute the preferred task with this abridged illustration.
Texture characteristics are extracted from DWT and irst order statistics using GLCM (Gray Level Cooccurrence Matrix). Most texture analyzes use GLCM approach. Using GLCM eliminates the image's second-order statistic texture functionality. GLCM is a tabulation of how much a picture displays various combinations of gray pixel rates. GLCM is sensitive to the texture samples being examined, and may reduce the number of gray concentrations. GLCM's most common textural characteristics are contrast, homogeneity, correlation, entropy, and strength.
Using DWT and GLCM, the segmented image draws 12 functions, and is applied in the categorization to    After extracting an item, the classi ier KNN is used for classi ication. KNN Classi ier, while de ining n neighbors as MA pixels, p = n / k determines the later likelihood of the query pixel being a MA pixel itself (Lu et al., 2018). Distance is calculated in optimized function space using Euclidean metric to locate nearest neighbors. The test process features are separated from an unknown image and provided to the classi ier for inding MAs. The irst-stage DWT results are shown in Figure 7.
The adjacent neighbor regulation tries to calculate approximately the subsequent probabilities of sample selection. A high k-value is optimal for consistent estimates. However, when every nearest k neighbors are near as much as necessary for problem collection, can the majority labels of their neighbors estimate their probability for posteriori. Therefore, a balance must be found that k's value relates to just a little portion of training samples.
The features of MAs and hemorrhages extracted for proposed DR recognition are shown in Table 1. First, color fundus images are resized to 640 x 480 pixels from different sources. Images are transformed to gray to reduce processing time. The photograph consists of three retinal fundus canals: red, green and blue. The big difference among blood vessels with background, besides the biggest difference among optic disk and tissue within the retina make the green channel of the RGB image slightly luminous and the choroid venous tissue visible before processing red channel. However, retinal vessels are transparent and show low contrast comparing to green channel. The gray image is utilized to locate optical disks. Blue channel is not used to distinguish, as it produces additional noise as well as fewer information regarding retina's morphological organization. Table 2 displays fundus images analysis and the quality of Random Forest Classi ier output images, including MAs and hemorrhages. Table 3 displays the accuracy of functions using SVM Classi ier.
The accuracy of the outcome of the proposed DR detection algorithm depends on the sensitivity and speci icity assessed. DIABET DB1 and ROC databases (Healthy and DR iles) took one hundred thirty images. Among these, 67 eye images were used as training samples, and 63 as test samples. Samples count is randomly selected in the test set and training set, giving the proposed algorithm robustness. The study samples amount is selected further than test samples, as shown in Figure 8 for better classi ication model preparation.
ROC displays a graphical plot showing a classi ier device's predictive potential as its degree of change of discrimination, as seen in Figure 9. Scheming true positive igure with false positive produces the ROC curve. Accuracy is the proportion of correct instances.
DR-affected retinal fundus picture is found to include nearly circular bright spots, including haemorrhages and MAs. However, only certain vascular residues exist in a healthy eye fundus image. The proposed DR detection system has 29, 28, 4 and 2 true positive, false positive, and false negatives. The system's reactivity and speci icity were calculated as 88% and 94% respectively. The results obtained from this method are compared to a few existing methods which claim to be better than the methods considered.

CONCLUSIONS
This article detailed an early detection method for DR from color fundus image for DR screening. The sensitivity and speci icity values obtained suggest that this system is suitable for detecting non-proliferative diabetes retinopathy. Here, a method for detecting MAs on retinal images was presented, with texture features analysis on the preprocessed image's segmented output. When considering only segmented tests for extracting and classifying features, the processed pixels count is greatly decreased. Five haemorrhage and MAs trained the DR detection model, making the proposed system robust and effective. This study has two main modules, one performing image segmentation, which is called the stage of candidate extraction, and the other performing KNN classi ication using Fuzzy C-Means (FCM) clustering algorithm. The reduction of wider blood vessels and further areas eliminates false positives. The proposed method reached 84% sensitivity at 5 false positives per picture. The technique may be extended to detect other anomalies like hemorrhage, exudates, etc. The device could also identify optical disks and blood vessels. Other classi iers will increase the method's sensitivity. Proposing a system for detecting PDR by taking abnormal blood vessels and cotton wools from color fundus images is the future work.

ACKNOWLEDGEMENT
I am thankful to all staff of Electronics & Communication/ Instrumentation Engineering department, Bharath Institute of Higher Education and Research for supporting this work.