Oral mucosal lesions in children with and without cleft lip and palate: a case control study

Yashila Periyasamy1, Vignesh Ravindran*2, Subhashini V C3 1Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77, Tamil Nadu, India 2Department of Paedodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 77, Tamil Nadu, India 3Department of Public Health Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 77, Tamil Nadu, India


INTRODUCTION
Cleft lip /cleft palate is known as a congenital craniofacial anomaly with cleft lip being recorded in 1556 by Pierse Franco, and cleft lip and palate was discovered in 1691 by Hendrik Van Roonhuyzez. Although the earliest evidence. Although the earliest evidence of it in antiquity was discovered in Egyptian mummies (Murray, 2002;Agbenorku, 2013).Due to the esthetic and functional defects, they lead a poor quality of life (Cooper, 2000;Jeevanandan, 2017). Cleft lip and palate is the second most common congenital birth defect in the world. The main aim of surgical correction of orofacial clefts is to help in feeding, guide facial growth, and improvise speech and language development (Burg, 2016;Govindaraju et al., 2017c).
Orofacial clefts are one of the common congenital craniofacial birth defects. They exhibit both ethnic and geographic variations (Mossey, 2009). Orofacial clefts are caused by various factors such as infection, toxicity, poor diet, hormonal imbalance and genetic interference. Genes play a key role in the formation of clefts (Jugessur et al., 2009). Incomplete fusion of these prominence during 4th to 12th week during intrauterine life leads to cleft lip, cleft palate or combination of both (Mossey, 2009;Szabo, 2012).
Oral mucosal lesions are abnormal alteration in colour, surface aspect, loss of integrity or swelling on the oral mucosal surfaces. Oral mucosal lesions are benign and they require no active treatment and symptomatic relief is only required. It interferes with mastication, swallowing and speech. It also causes burning, irritation and pain during food consumption (Zainab and Salih, 2012;Jeevanandan and Govindaraju, 2018). The oral mucosal lesion could be due to viral, bacterial or fungal infections, local trauma or irritation and systemic diseases (Feng, 2015;Kaul, 2017). A study conducted (Chandroth, 2014;Govindaraju et al., 2017b) concluded that 28.9% of children with cleft defects had some kind of oral mucosal lesion. He also concluded that oral candidiasis was the most common, followed by traumatic lesions and erythema multiforme (Neville, 2015;Ravikumar et al., 2017). Traumatic oral soft tissue lesions due to habits that tend to cause severe injuries such as lip and cheek biting, orthodontic appliances, food burns, sharp edges of wires in space maintainers (Neville, 2015;Lakshmanan et al., 2020). There is surprisingly little information on the prevalence of oral mucosal lesions and extraoral lesions among patients with cleft lip and palate in India. Thus the aim of the present study was to assess the presence of oral mucosal lesions in children with and without cleft lip and palate.

MATERIALS AND METHODS
This retrospective study was conducted under a hospital-based university setting. Ethical approval for this study was granted by the institute's ethical committee (ethical approval number: SDC/SIHEC/2020/DIASDATA/0619-0320).
Consent to use treatment records for research purposes were obtained from patients/guardians at the time of patient entry into the university for dental needs. The retrospective data were collected by obtaining and analysing the 89000 dental case records of the university from June 2019 to March 2020. We registered our research topic in a private dental institute in Chennai. Our project was evaluated and approved by the ethical committee of the private dental institute. The sample size that we have collected from the private dental institute was 30 (20 present -cleft lip and palate and ten absent -cleft lip and palate). The inclusion criteria were children with cleft lip and palate only, children between the age of 0 -17 years, complete photographs.and written records regarding the complete intraoral examination of the patient. Age and gender-matched controls, i.e. children without cleft lip and palate were taken according to the relevant cases obtained from the inclusion criteria. The exclusion criteria were incomplete and censored dental records and the absence of photographic evidence of cleft lip and palate and clinical indings. The selected case and control group were examined by three people; one reviewer, one guide and one researcher. The patients' case sheets were reviewed thoroughly.
Cross-checking of data including digital entry and intraoral photographs was done by an additional reviewer and as a measure to minimise sampling bias, samples for the group were picked by simple random sampling. A digital entry of clinical examinations and intraoral photographs of selected subjects were assessed and this included the assessment of the oral mucosal lesion in children with cleft lip and palate as mentioned before by the examiner based on intraoral photographs and clinical examination data for each tooth. The examiner was trained to add data of malocclusion as present or absent for both case and control group by tabulation using excel software and even the type of malocclusion was noted. Data analysis was done using SPSS PC Version 23.0 (IBM;2016) software for statistics. The prevalence of malocclusion for both case and control group were compared by Mann Whitney Test.

RESULTS AND DISCUSSION
The inal study population was 20 patients with ten patients in the case group (children with cleft lip and palate) and ten patients in the control group (children without cleft lip and palate)- Figure 1 shows that Notice the equal distribution of cases for both the case group and control group. In the case group, out of the ten patients, 5 (50%) were males and 5 (50%) were females. A similar distribution was done while selecting the control group- Figure 2 shows that Notice the equal distribution of gender for both the case group and control group. There is an absence of oral mucosal lesion in all the cases in both groups (children with and without cleft lip and palate) Figure 3 shows that None of the cases in both the case group and control group had oral mucosal lesions. However, comparing these data with the Mann-Whitney U test was statistically signi icant (p-value=0.000), there were no cases in both the groups with the presence of oral mucosal lesions.
Oral mucosa acts as a protective barrier against trauma, pathogens and carcinogens (Subramanyam, 2018). Oral cavities can be considered as a gateway into the digestive system. Sometimes, oral lesions are the irst indication of systemic disease (Majorana, 2010;Ali et al., 2013). (Pinto et al., 2014) divided paediatric soft tissue oral lesions into several categories: developmental lesions, white lesions, red and/ or white lesions; brown-black lesions; soft tissue nodules-in lammatory/ reactive lesions; ulcerations and benign tumours (Pinto et al., 2014;Govindaraju, 2017). Bezerra et al. (Bezerra and Costa, 2001) found that childhood oral mucosal lesions among 104 patients ranging from 0 to 5 years children to be 2.3% by observing their dental records. According to this study, the most common oral mucosal lesions were Bohn nodules (37%) followed by candidiasis (25%) and benign migratory glossitis were the least common with 21%. Bessa et al. (Bessa et al., 2004) found that incidence of childhood mucosal lesions among 0 to 4-year-olds to be 24.9% and the most common lesions were geographic tongue (9.8%) followed by bite injuries (6.1%). The most common lesion was caused by Candida albicans.
According to Maxill (Morrill, 2005), the use of feeding bottle led to oral bacterial colonization. The bottle acts as a vector for the candida in the environment. Traumatic ulcers develop from physical, thermal or chemical injuries. Accidental biting during mastication or consumption of hot food may cause traumatic ulcerations (Panchal et al., 2019). Iatrogenic damages caused by dental treatments also cause traumatic ulceration (Anura, 2014;Gurunathan and Shanmugaavel, 2016). Frictional keratosis also called mastico buccasum are white patches caused due to traumatic tooth brushing (toothbrush keratosis) due to constant rubbing of the mucosa, frictional keratosis can occur (Shulman, 2005;Christabel, 2015).
Leukodema is a white lesion which is found on the buccal and/or the labial mucosa (Ali et al., 2013;Packiri, 2017). It can be unilateral or bilateral and is associated with smoking and local irritation. Prevalence differs from 0.96% to 58% and highest prevalence noted in African populations (Pindborg, 1965;Martin, 1973).
In our study, we found that children with a palate as well as children without cleft palate both had an absence of oral mucosal lesions with the p-value >0.05 is not signi icant. There were a few contradicting studies by (Chopra, 2014), which found that children with clefts have a higher prevalence of anterior open-bite, increased overjet and presence of oral mucosal lesions (Chopra, 2014;Ünür et al., 2015) stated that issured tongue was the most frequent lesion followed by traumatic ulcer. The consensus of our study disagreed due to the smaller sample size as well as the geographic restrictions present while carrying out this study.
The advantages of the study were that this was a case-control study with age and gender-matched control to provide better results and high internal validity. The limitations found in the study are geographic restrictions as the patients are from around the same region. Besides, there was only a single ethnicity as a group of people who are from the same ethnicity group. The unicentric study, small sample sizes and indirect clinical observation are also some of the challenges faced while conducting our study. The future scope of this study could involve studies with a larger sample size for the case group, that is not con ined to a particular geographic region.

CONCLUSIONS
Within the limitations of the study, oral mucosal lesions were not identi ied in children with and without cleft lip and palate. Despite the presence or absence of cleft, the dental practitioner should be vigilant in noticing such lesions and treat it accordingly at the earliest for the betterment of the patient.