Awareness of Medical Applications of OzonatedWater Therapy Among Dental Students

Ozone (O3) happens to be a triple atomic particle that comprises three oxygen iotas with an atomic load of 47.98 g/mol. Ozone is a thermokinetic coseismal particle which, contingent upon framework conditions like temperature and weight, has a short half-life and breaks down into sub-atomic oxygen (O2). Ozone is utilized asmedication inmany conditions. The study aimed to assess the awareness of medical applications of ozonated water therapy among dental students. A cross sectional study was done with a self-administered questionnaire with 10 questions circulated among 100 dental students. The questionnaire assessed the awareness about ozonewater therapy inmedical applications, their medicinal uses, antiin lammatory activity, mechanism of action and side effects. The responses were recorded and analysed.87% of the respondents were not aware of medical uses of Ozone water therapy .73% were not aware of antiin lammatory activity of Ozone water therapy.83 % were not aware of themechanism of action of Ozonewater therapy .85%were not aware of side effects of Ozonewater therapy. The awareness about the use of Ozone water therapy in medical applications is very less among dental students. Increased awareness programs and sensitization and continuing dental education programs along with greater importance to the curricular modi ications should be incorporated to improve the awareness levels.


INTRODUCTION
Ozone (O3) happens to be a triple atomic particle that comprises three oxygen iotas with an atomic load of 47.98 g/mol. Ozone is a thermokinetic coseismal particle which, contingent upon framework conditions like temperature and weight, has a short half-life and breaks down into sub-atomic oxygen (O2). Ozone is utilized as medication in many conditions (Bocci, 2002;Burns, 1997). This O3/O2 blend displays different consequences for the invulnerable framework, for example, regulating the phagocytic movement of alveolar and peritoneal macrophages. O3 builds the movement of cancer prevention agent proteins including superoxide dismutase (SOD), catalase and glutathione peroxidase, accordingly setting up the host to confront pathophysiological conditions interceded by reactive oxygen species (ROS) (Bocci et al., 2011a,b).
Discoveries of past examinations have demonstrated that delayed inhalation of O3 gas harms the respiratory framework and other organs (Aris et al., 1993;Balmes et al., 1996). A progression of metaexaminations and assessment of geographic and occasional Ozone hazard has given proof to the relationship between O3 gas and mortality rate (Devlin et al., 1991). As O3 inhalation is joined by perilous symptoms and since O3 is a gaseous state of matter, its advantages are constrained. To build the adequacy and wellbeing of ozone treatment, ozone might be disintegrated in water (Murphy, 2009). This is pro itable as ozonated water is simpler to oversee and more secure than ozone gas. In any case, gadgets to create ozonated water, which can absolutely direct the convergence of the disintegrated ozone gas are not savvy. In this manner, ozonated water is not generally used (Harber et al., 1996;Krishna et al., 1998).

Ozonated
water possesses antimicrobial effects (Scott and Lesher, 1963). Ozmen et al. (1993) reported that ozonated saline was effective as a medical aid for treating empirical peritonitis in rat models with several bene its. Hence this study was done with an aim to assess the awareness of medical applications of ozonated water therapy among dental students.

MATERIALS AND METHODS
A cross sectional study was done with a selfadministered questionnaire with 10 questions circulated among 100 dental students. The questionnaire assessed the awareness about ozone water therapy in medical applications, their medicinal uses, anti in lammatory activity, mechanism of action and side effects. The responses were recorded and analysed.

DISCUSSION
Oxidative harm to fundamental cell structures, for example, DNA, lipids, proteins and ilms is initiated by ROS. The mitochondrial electron transport chain is a signi icant wellspring of intracellular ROS and is helpless to harm started by ROS. ROS conceivably actuates cell cancer prevention agent protection catalysts, including SOD. Already, it has been demonstrated that introduction to LPS increments coursing neutrophils prompting foundational oxidative pressure . Tissue neutrophils, monocytes and macrophages discharge in lammatory cytokines including TNF-α. Past reports demon- strate O3 has solid cancer prevention agent exercises (Lippmann, 1989). In the current examination, the serum level of TNF-α and SOD serum action were essentially diminished and expanded, separately, in the ozonated water-treated contrasted with different groups. In lammatory cytokines, including TNFα, are signi icant in lammatory intercessor in peritonitis (Krishna et al., 1998;Turrens, 2003).
Since O3 treatment incites oxidation in the body, the exercises of cancer prevention agent compounds, for example, SOD are expanded resulting to O3 treatment. In the test septic rodent model, the remedial impact of O3 treatment in the rectum was af irmed. Our outcomes show the ozonated water additionally display antioxidative and calming exercises. Past investigations demonstrate ROS was delivered following endotoxin shock-activated nuclear factor (NF)-κB and expanded the creation of in lammatory cytokines. Overexpression of SOD smothers TNF-α creation in human mammary cancer cells (Krishna et al., 1998;Schat et al., 2012). The awareness about clinical applications of ozone water is less among dental understudies. In any case, extra investigations ought to be performed to increase the comprehension of the component associated with the clinical impacts of ozonated water.

CONCLUSION
The awareness about the use of Ozone water therapy in medical applications is very less among dental students. Increased awareness programs and sensitization and continuing dental education programs along with greater importance to the curricular modi ications should be incorporated to improve the awareness levels.

Funding Support
The authors declare that they have no funding sup-port for this study.