Knowledge and Awareness Regarding Protocol After Needle Stick Injury among Medical Students

Needle stick injuries are more prominent among health care professionals, and it can be de ined as a wound of percutaneous piercing by a needle point or other sharp instruments and objects. Needle stick injury (NSI) is a significant risk towards the transmission of pathogens and diseases. This present study was aimed to assess the knowledge, awareness and prevalence of NSI amongmedical students. This is a cross-sectional study conducted among the medical students (104 student). The questionnaire was distributed through an online survey google forms among medical students to assess their knowledge and awareness towards the NSI. SPSS software was used to analyze the collected data. The Pearson Chi-square test was done in association with the year of study of the respondents. The survey results showed that 60.7% of the students were aware of the disease caused by the NSI, 35.5% students were aware of themeasures to be takenwhenNSI occurs, 63.6%of the students said single handed technique is safe for needle recapping. The chi-square analysis showed that there is no association between the year of study and awareness on the protocol to be followed after NSI. The study revealed that knowledge about NSI and the preventive measures among medical students was inadequate. Hence, it is utmost important to make the medical students aware of the protocol for NSI and to train them on that.


INTRODUCTION
Needle stick injury (NSI) can be described as a wound of percutaneous piercing caused by a needle point, and also by other sharp instruments and objects. The health care professionals are exposed to various type of infections which are affected by microorganisms and viruses like hepatitis B and hepatitis C viruses (Gupta et al., 2019;Mp, 2016). Not only the infectious diseases, the prevalence of non-communicable diseases like metabolic disorders (Ponnulakshmi et al., 2019;Shukri et al., 2016), various types of cancers like breast cancer (Gan et al., 2019), hepatic carcinoma (Jainu et al., 2018), laryngeal cancer (Wang et al., 2019), oral cancer (Rengasamy et al., 2018;Ramya et al., 2018), and thyroid cancer (Ma et al., 2019) etc., are also increasing globally nowadays. Various procedures like recapping of the needle, injuries occurring at the operating room, suturing etc are the reasons for NSI. There are many ways that disease can be transmitted, depending on the needle and the pathogens loaded in it. Previous articles revealed that every year around 3 million individuals got injured by NSI (Gambhir et al., 2013). Maximum care and precautions should be required to handle the needle or sharp objects. Improper handling of needles can cause various health disorders. Most of the NSIs happen either during the usage or disposal of sharps. Hence the proper disposal of the sharps is very important, otherwise they might get into the garbage and can injure the health workers which can transmit various diseases (Bindra et al., 2014) At one single prick more than 20 different pathogens can be transmitted by needlestick injuries. Laboratory personnel and housekeeping are at the highest risk of incidence of NSI, but about 60% of the injuries occurred in nursing personnel (Saini, 2011).
There are some standard protocols to be followed once the NSI occur. These are the measures to be followed:the initial step to be followed is immediate washing of the injury site with soap and water. The next step is to report the incident and an exposure report sheet completed. It is important to assess the exposure (type of luid, type of needle, amount of blood on the needle, etc), the exposure source should be evaluated: 1. HIV, HBV, and HCV status of the patient, 2. If the status is unknown, consent and testing of the patient for these diseases, 3. If the patient is not available to be tested, the likelihood of infection based on the community served by the hospital. Proper management of any positive exposure is of utmost important (Penalver, 2005) The medical and dental students are more exposed to NSI due to their consequences and relative inexperience, ignorance and lack of awareness of NSI. According to WHO the annual estimate exposed to the pathogens globally is 2.6%, 5.9%,0.5% for HCV, HBV, HIV respectively. Sixty-six thousand infections were affected by sharp injury exposures worldwide. To reduce NSI, further precautions should be taken by the medical students (Firoz et al., 2018;Megnath et al., 2017).
Previously numerous studies have been conducted by our team in various ields. Studies have been conducted in herbal medicines (Menon et al., 2016;Chen et al., 2019), active constituents (Li et al., 2020;Sk and Jainu, 2014), nanoparticles (Wu et al., 2019;Ke et al., 2019) and protein characterization (Rengasamy et al., 2016). The aim of the present study is to assess knowledge and awareness of NSI among medical students.

MATERIALS AND METHODS
This is a cross-sectional questionnaire survey conducted among medical students. A random sam-pling method was used for the study. A total of 104 students have responded to the survey. Participants have been randomly enrolled in the online survey through google forms. A validated questionnaire consisted of 20 closed ended and yes/ no questions distributed to assess their knowledge, attitude and awareness on NSI. The responses have been taken from google forms and were analysed by SPSS software. The Pearson Chi-square test was done in association with the year of study of the respondents. The results were expressed as pie charts and bar diagrams.

RESULTS AND DISCUSSION
The results of the present survey are as follows: In our study (Figure 1) about 66.4% of the participants were male, and 33.6% were females. 66% of the respondents were male (Red), and 34% were female (Blue). Figure 2 shows that 59.8% of the medical students were affected by the NSI, and 40.2 % of the medical students were not affected by NSI. Figure 3 shows the year of study of the respondent where 26% are the irst year, 23% second year and 26% third year, 30% the fourth year, 69.2% of the students were aware of the protocol to be followed after NSI and 30.8% of the students were not aware of it ( Figure 4). Figure 5 shows that 32.7% students said that NSI is caused due to passing of a sharp object, 23.4% said due to recapping the needle, 14% said due to the suturing, 29.9% said all these which are mentioned above. Figure 6 60.7% of the students were aware of the disease caused by NSI 39.3% of the medical students were not aware of it. When students were asked about the disease caused by NSI 43.9% students said HIV can be transmitted through NSI, 28% students said Hepatitis B, 15% of the students said Hepatitis C, 12.1% of the students said Tuberculosis can be affected (Figure 7). Figure 8 shows that 63.6% of the students use single handed technique for needle recapping, 36.4% use double-handed technique for needle recapping. Figure 9 shows that 70% of the participants wear gloves while they are treating the patients and 30% of the participants do not. When the students were asked about the measures to be taken after NSI, (Figure 10) shows that 35% of the students wash the site with the water, 15% of the students squeeze the blood, 14% of the students wash the site with soap and water. Figure 11 shows that 64.5% of the medical students are vaccinated against Hepatitis B, 35.5% of the medical students have not been vaccinated.
Association of the study year of participants and regarding the awareness of protocol of NSI and        (Figures 12, 13, 14, 15 and 16). The statistical analysis showed that there is no association between the awareness and study year of the respondents. Figure 12 shows the majority of the 4th year students (19 participants) were aware of the universal precaution guidelines, which is statistically not signi icant (Pearson chi-square value = 0.744, p-value = 0.856 >0.05). Figure 13 shows there is no association between the year of the study and responses regarding the common clinical activity which causes   NSI (Pearson chi-square value = 11.753 p-value = 0.228 >0.05). Figure 14 shows there is no association between the year of the study and responses regarding the awareness of the diseases caused by NSI (Pearson chi-square value = 6.421, p-value = 0.093 >0.05). Figure 15 shows there is no association between the year of the study and responses regarding the safest technique for NSI (Pearson chisquare value = 5.291, p-value = 0.152> 0.05). Figure 16 shows there is no association between the   year of the study and responses regarding the measures of NSI (Pearson chi-square value = 8.573, Pvalue = 0.478> 0.05).
In the previous study, the results showed that 80% of the HIV disease can be reduced by using Antiretroviral Medications (Wilburn and Eijkemans, 2004). The study showed that the majority of the respondents have been vaccinated against the dis-   ease caused by NSI (Hakeem et al., 2019), which is similar to our study. Majority of the NSIsare occurred by Dental professionals where they are at high risk Callan et al. (2006). In another study, the author reported 19 indications about the NSI, the majority of the participants were aware of it (Rabbitts, 2003). To avoid NSI preventive strategies, have to be taken. All the medical students must be vaccinated against Hepatitis virus, and awareness on the protocols after NSI should be created. Another study explains that 53.4% of injuries occurred before and after the disposal of sharp objects, 63% injury due to recapping of the needle (Jahan, 2005), which is similar to our study. The study by Guruprasad and Chauhan showed that 11% of the participants has been affected by diseases, through infected needles. 67.5% of the respondents were not aware of the technique for needle disposal (Guruprasad and Chauhan, 2011). Another study showed that most common clinical activity to cause NSI are due to blood collection (55%) and then due to suturing (20.3%), (11.7%) due to vaccination, by needles recapping (66.3%) and needle bending before discarding (11.4%) (Muralidhar et al., 2010). Most of the cited articles are similar to our indings.

CONCLUSIONS
This study revealed the knowledge and awareness of the protocols to be followed after a NSI and the preventative measures was inadequate among the medical students. Only very few students (14%) were aware of the measures to be taken immediately after NSI, although the majority of the students (64%) were aware that single handed needle recapping is the safest method. The survey also shows that there is no association between the year of study and knowledge and awareness of the protocol of NSI. Hence measures to be taken to make the students aware of NSI and to train them on the protocol to be followed.

ACKNOWLEDGEMENT
The author expresses their sincere thanks to Saveetha Dental College for extending full support to complete this study.