Antioxidant activity of silver nanoparticles synthesis using Cinnamomum verum and Phyllanthus emblica formulation

Nanotechnology is de(cid:977)ined as a very broad (cid:977)ield of science which is as diverse as surface science, organic chemistry, molecular biology, semiconductor physics, molecular engineering etc. Nanotechnology might have the option to make numerous new materials and devices with a huge scope of use, for example, in Nano medicine, Nano electronics, and bio materials energy cre-ation. Phyllanthus emblica is a remarkable supply of diet C; consequently, it facilitates increase your immunity. Its dietary pro(cid:977)ile additionally comes stud-ded with a variety of polyphenols which are acknowledged to combat towards the improvement of most cancers cells. Cinnamomum verum is stocked with powerful antioxidants which protects the body from oxidative damage caused by free radicals. The physical characterization of these silver nanoparticles was veri(cid:977)ied using UV - visible spectroscopy and the surface Plasmon reso-nance band was formed at 400nm of silver nanoparticles. The silver nanoparticles synthesized using Cinnamomum verum and Phyllanthus emblica extract showed higher antioxidant activity.


INTRODUCTION
Phyllanthus emblica, which belongs to the family Phyllanthaceae, is also known as emblic, emblic myrobalan, myrobalan, Indian gooseberry, Malacca tree or amla. This extract from these fruits has been utilized in traditional medicine to treat symptoms ranging from constipation to the treatment of tumors (Unander et al., 1990). Phyllanthus emblica extracts have been found to have antitumour properties against certain tumours (Ngamkitidechakul et al., 2010), and antioxidant, antidiabetics, hypo-lipidemic, antibacterial, gastro protective and chemo preventive properties (Mirunalini and Krishnaveni, 2010). The small, tropical, evergreen tree most noted for its bark, Cinnamomum velum or Cinnamomum zeylanicum, provides the world with the most widely known spice, cinnamon. The scienti ic name is Cinnamomum zeylanicum; it is one of the most important and common spices used not only for cooking and modern medicine. In general, among the cinnamon genus, more or less 250 species have been described, with trees scattered around the world (Sangal, 2011;Vangalapati et al., 2012).
Nanotechnology can be described as the engineering and science elaborate in the synthesis, design, application of materials and characterization and devices whose compact, functional organization, in at least one dimension, is on the nanometer scale or one of a billionth of a meter (Agarwal et al., 2017). Tokyo Science university was irst described the "Nanotechnology", Norio Taniguchi in 1974 (Santhoshkumar et al., 2017). These technologies encompass protein arrays, Nano pore technology, Nano arrays, nanoparticles (NPs) as an expedient in immunoassays and Nano sensors, among others. Quantum dots (semiconductors) and gold NPs are the most extensively used, but new materials are accessible as more molecular entities are ind as amenable to Nano scale design and fabrication. The expanse of a phenomenon is Nano biosensors, in which antibody-based piezoelectric Nano biosensors are well maturing (Menon, 2017;. The currently being developed application of nanotechnology in medicine requires the use of nanoparticles to transmit drugs, heat, light or other substances to speci ic cell types (such as cancer cells). This approach prevents damage to healthy cells in the body and helps diseases to be identi ied sooner. (Agarwal et al., 2018).
Silver nanoparticles are nanoparticles of sliver size ranging from 1 nm to 100 nm. Although often described as 'sliver,' some are made up of a large percentage of silver oxide because of their large surface to bulk silver atom ratio (Santhoshkumar et al., 2019). The properties of human-suitable silver nanoparticles are being studied in laboratory and animal studies to determine potential effectiveness, toxicity and cost. Silver nanoparticles have shown excellent bactericidal properties against a wide variety of microorganisms. (Rajeshkumar, 2016;Rajeshkumar and Malarkodi, 2017). As indicated, the physiological function of antioxidants is to prevent damage to cellular components that occur as reverberations of chemical reactions involving free radicals. Rajeshkumar et al., 2016). The redox reaction of Ag+ requires a reducing agent. They are used in wound dressings as antimicrobial agents, as topical creams to stop wound infections Rajeshkumar and Bharath, 2017) and as anticancer agents. (Rajeshkumar and Naik, 2018). Therefore, in this study, we evaluated the antioxidant activity of silver nanoparticles synthesis using Cinnamomum verum and Phyllanthus emblica.

Preparation of fruit extract
Fresh Cinnamomum verum and Phyllanthus emblica extract powder was purchased from herbal health care Centre. 0.5 gm of Cinnamomum verum and Phyllanthus emblica fruit powder was added to 50ml of distilled water to the conical lask and boiled for 7 to 8 minutes in the heating mantle. The boiled extract was iltered using a ilter paper.

Synthesis of nanoparticles
0.0169g of sliver nitrate is added to 90ml of distilled water and 100ml of plant extract. The plant extract of the solution is kept in the shaker. The colour change is observed after 2 hours for 3 days for analysis of the synthesis of nanoparticles. The nanoparticle synthesis is measured using the instrument of double beam spectrophotometer. This mixture is centrifuged for 10 minutes and nanoparticles are settled down is taken out with the help of pellet.

Application of antioxidant
Each extract (10-50 µg / ml) in water and ethanol is mixed with 1 ml of methanol ice solution containing DPPH free radicals to determine the scavenging potential for DPPH. Until measuring absorbance at 517 nm, the mixture solution was shaken vigorously and left to stand for 20 minutes in the dark. The scavenging potential was then determined using the equation.

RESULTS
The formation of the nanoparticles began after the extract was combined with the silver nitrate solution. The apparent colour shift of the solution from light brown to dark brown and the formation of silver nanoparticles was con irmed by spectral analysis. It con irmed the AgNPs and vitamin c has the antioxidant activity.

DISCUSSION
In the present study, the synthesis silver nanoparticle from Cinnamomum verum and Phyllanthus emblica extract was elementary. Our study showed a signi icant percentage of inhibition as the concentration of the extract increases when compared to the standard vitamin C which may be due to the presence of lavonoids and phenolic compounds that is responsible for its antioxidant activity and also it's reducing power. Our results clearly demonstrate the ability of Amla and cinnamon based AgNPs to scavenge free radicals which could be attributed to the presence of functional groups present on the surface of nanoparticles. The same antioxidant property was also demonstrated by V. Ravichandran et al. with the AgNPs synthesized using Parkia speciosa leaves. Free radicals are known to have a de inite role in a wide variety of pathological manifestation which is combated by the antioxidants.
The colour change of the solution has been observed from light brown to dark brown after 3 days (Figures 1 and 2). The UV-vid spectrometer readings were recorded, the peak at 400nm con irms the nanoparticle synthesis. The antioxidant activity of Cinnamomum verum and Phyllanthus emblica was compared with the activity of Cinnamomum verum and Phyllanthus emblica by DPPH method. The concentration of (10,20,30,40,50) µl the absorbance is (0.223,0.197,0.263,0.534,0.603).
The previous studies shows, that the silver nanoparticles synthesized using various plants, algae and bacteria having signi icant antimicrobial, antioxidant and anticancer activities (Jeevitha and Rajeshkumar, 2019;Rajeshkumar, 2017).

CONCLUSIONS
Cinnamomum verum and Phyllanthus emblica based silver nanoparticles has antioxidant activity against pathogen by scavenging free radical. Antioxidant minimize damage the cells from oxidants and can help ight against ageing, cancer and chronic disease like cardiovascular disease. In dentistry, it can be used as toothpaste, tooth powder and mouth wash.

Funding Support
The authors declare that they have no funding support for this study.