Gastroprotective effect of leaves of Breyniavitis-Idaea

Poojitha M*1, Saravanakumar A S2, Satyanarayana S V3 1Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu, Andhra Pradesh, India 2Department of Pharmacology, Sri Venkateswara College of Pharmacy, RVS Nagar, Chittoor, Andhra Pradesh, India 3Department of Chemical Engineering, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu, Andhra Pradesh, India


INTRODUCTION
PUD (Peptic Ulcer Disease) distresses most of the people in the world and disabling their daily activ-ities. It is noted as one the commonest and stubborn diseases that occur in people and affect their lives (Antonio et al., 2004). There are various factors involved to cause the disease such as oxidative stress, chronic and excessive smoking, malnutrition and due to chronic use drugs like NSAID's and steroids. The imbalances in the offensive and defensive factors like HCl, enzymes, H. Pylori and Mucin, prostaglandins NO2 and carbonates and bicarbonates act as common factors in causing the PUD (Baggio et al., 2003;Chiang et al., 2005;Cho and Ogle, 1979;Cho et al., 1976).
One of the major setbacks of the PUD treatment is the incidence and development of drug tolerance and others being relapse of disease, noting of side effects on clinical observation after treatment.  These draw backs of synthetic drugs make the ef icacy of drugs quite arguable. This triggered the scope for search and development of newer drugs that treat PUD. Herbal drugs were seen as potential alternatives for the treatment of PUD without showing side effects and equaling the treatment ef icacy (Danielsson et al., 2003;Fellenius et al., 1981). Following the literature review on investigations of antiulcer activity of various plant drug, the current study was used to explore the antiulcer potential, chemical elements present in the methanol extract of leaves of Breynia vitis-idaea., (Family: Poaceae) and the same is being reported here (Flemstrom et al., 1982).

Collection and Authentication of the plant material
The plant part of Breyniavitis-idaea (Family: Phyllanthaceae) leaves were obtained from Chittoor District, validated by Dr. Madhava Chetty, Asst Profes-   sor, Department of Botany, Sri Venkateswara university, Tirupati 517502, A.P., India.

Animals Used
Albino rats of either sex weighing 150-200g were kept in a 12 h light or dark cycle at a constant moderate thermal condition at 25 • C with free access to libitum.

Acute toxicity study
Acute toxic dose of methanol extract of leaves of Breynia vitis-idaea., (Family: Poaceae) were determined following OECD guideline no. 423 (Acute Toxic Class Method). Found that the test extracts weren't deadly or toxic to the experimental animals was smooth at 2000mg/kg dose.

Ulcer inducing models
Ethanol Induced Gastric Ulcer 8ml/kg of alcohol was administered to all the assemblies of rats excluding the assembly 1 for which is well thought-out as normal healthy group (Grijalva and Novin, 1990;Gupta et al., 1985). The ulcer catalog for to each rat was taken as the mean ulcer score (Hase and Moss, 1973).

NSAIDS (Indomethacin) Induced Gastric Ulcer
Ulcers lesions in the stomachs of experimental animals were induced with the help of indomethacin (40 mg/kg p.o) administered to all groups after fasting for 24h (Hoogerwerf and Pasricha, 2001).

Pyloric Ligation Induced Gastric Ulcer
This procedure was not performed on normal healthy group (group 1). After 4h from surgery, animals were forfeited through cervical dislodgment and score of ulcers was noted on the dissevered stomachs (Kahraman et al., 2003).

Measurement of ulcer index
Ulcer index (UI), and percentage of inhibition of ulcer lesions (%I) were calculated as described in (Pillai and Santhakumari, 1984).

Statistical analysis
All the numbers were articulated as mean ± standard error mean (SEM). Implication of variances among the group was calculated by using ANOVA.

RESULTS AND DISCUSSION
The methanol extracts of leaves of Breynia vitisidaea showed a certainly defensive effects contrary to ulcers persuaded by alcohol, NSAIDS (indomethacin), Pylorus ligation and cold restraint models. The release of free radicles is the mechanism seen in ethanol induced ulcers which can be further supported by its improved activity in pylorus ligated method (Sasajima et al., 1978). The extract valor has also had an asset to rise prostaglandins thereby constructing a mucosal layer for shielding the ulcer from any direct exposure to the acid which is apparent from indomethacin induced gastric ulceration model (Shah et al., 1997). The NSAID drug is a COX inhibitor which disrupts the membrane integrity and causing rupture in the low of blood. The extract might have helped in production of endogenous prostaglandins for protecting the ulcers (Shay et al., 1945;Singh and Majumdar, 1999;Szabo et al., 1985). Gastric ulceration made by cold restraint stress is undoubtedly facilitated by the upsurge in gastric motility, degranulation of mast cells, decreased gastric mucosal blood, lowering in the prostaglandin synthesis and excretion of glycoproteins in the mucus (Taira et al., 2005;Valle, 2005;Warrier et al., 1995;Weiner, 1996). Any of the above factors could have played an important role in the formation of ulcers. Finally, the extract was proven to have antioxidant activity and it is proven successful in thwarting the ulcers produced due to the physical strain or stress which produces reactive oxygen species detrimental to the stomach lining (Yamamoto et al., 1992).

CONCLUSIONS
MEBV has exhibited dose reliant inhibition in ethanol induced stomach ulcer lesions and stimulate the secretion of prostaglandins or possess prostaglandins like-substances reveals that the as a potent inhibitor of stomach mucosal abrasions caused by ethanol, indomethacin, pylorus ligation and cold-restraint stress in rats. Further, our results support the ethnopharmacological claims of Breynia vitis-idaea as potent anti-ulcer agent. Etiologies of ulcers produced in different ulcer models are different. The study concluded that MEBV and its dynamic constituents may emerge as more effective healing agent to counter gastric ulcer.