EXAMINATION OF CREATININE AND PROTEIN LEVELS GIVEN ROYAL JELLY SUPPLEMENT

The aim of this study was to determine the effect of royal jelly on Creatinine and Protein levels in 1000 mg/day dose smoking addicts with controlled experimental design. For this purpose, Fagerström Nicotine Dependence Scale was applied to smokers, a total of 20 adult male cigarette addicts (n=10) with high addiction scores (6-7 points) were included in the study and the cigarette addict experimental group (n=10) taking royal jelly supplementation. Criteria such as smoking and dependence of the individuals included in the study, not having any disease and not having allergies to honey derived foods were determined and groups were formed in this way. The experimental group was given liquid royal jelly (n = 10/1000 mg /day) supplemented in glass vials in the refrigerator at the same time every morning for three weeks. No application was made to the control group. In order to determine Creatinine, Protein levels in both groups, blood samples were analyzed one day before and one day after the study. SPSS 22.0 package program was used for statistical analysis of measured data of supplementation and control groups, 2x2 mixed factor ANOVA and LSD tests were performed. Statistical results were evaluated at p <0,05 significance level. In the analysis of the levels of Creatinine and Protein between the pre-test and post-test of the supplementation group receiving royal jelly supplementation and the non-supplemented control group, no statistical significance was found (p>0,05). In the intergroup analysis of the two groups, again no significant difference was found (p>0,05). As a result of this study, it can be said that royal jelly supplementation at a dose of 1000 mg/day for 21 days does not affect Creatinine, Protein levels in cigarette addicts.


INTRODUCTION
It is emphasized that the increased risk of cardiovascular diseases, cancer and respiratory diseases due to smoking may be related to differences in lifestyle behaviors and eating habits in smokers (Yılmaz and Aykut, 2012). Nutritional habits of smoking individuals compared to other individuals; some vitamin and mineral levels may change because it is different. Looking at the studies; the intake of vitamin C, folic acid, pulp, vitamin A, polyunsaturated fatty acids, iron, carotene and vitamin E was found to be lower and saturated fatty acid intake was higher in smokers than non-smokers (Jitnarin, Kosulwat, Boonpraderm, Haddock & Poston, 2008;Yılmaz & Aykut, 2012). When the consumption of food is examined, individuals who smoke are consuming more white bread, tea sugar, red meat, butter, whole milk, eggs than non-smokers; they consume less whole wheat bread, high pulp breakfast cereals, vegetables and fruits (Yılmaz & Aykut, 2012). When the studies are examined; it is emphasized that correct nutritional supplements and physical activities increase the quality of life of individuals and have psychological and psychomotor (Alıncak, 2016a;Alıncak, 2016b;Alıncak, 2017) mandibular glands of worker bees for feeding the queen bees, water, protein, sugar, fatty acids, free amino acids, minerals iron and calcium and vitamins are a food rich in thiamine, niacin, riboflavin (Taniguchi, Kohno, Inoue et al., 2003;Okamoto, Taniguchi, Kunikata et al., 2003). It is reported that royal jelly, which is widely consumed by humans, contains very low amounts of biologically active substances such as ptrein, neopterin, biopterin, xantopterin and hormones due to the thought that it provides vigor, vitality and contributes to cell renewal (Akyol, 2013;Rembold and Dietz, 1965). In many studies on royal jelly, it suppresses humoral immunity in rats, stimulates proliferation and antibody production of immune competent cells in mice (Sver et al. 1996), increases hemopoietic origin cell production , and decreases cholesterol level. effects .
In addition to these, in human studies; it has been emphasized that it has cell repairing and rejuvenating effects along with its therapeutic role in skin and hair diseases, regulatory effects of sexual functions (Akyol, 2013;Yatsunami and Echigo, 1985). In this study; It was wondered that royal jelly, which is a rich and natural food source, will affect the Creatinine and Protein levels of cigarette addicts.

Subjects
The study protocol was explained to all participants prior to the study and voluntary participation certificate was obtained from the participants. A total of 20 healthy sedentary smokers aged 20-25 years participated voluntarily.

Experimental Design
This is an experimental design study. A total of 20 men were randomly divided into two groups. For this purpose, the Fagerström Nicotine Addiction Scale was administered to smokers and those with advanced addiction scores (6-7 points) were identified. A total of 20 adult males; smoking addict control group (n = 10) and royal jelly supplement (n = 10) were included in the study. Criteria such as smoking and dependence of the individuals included in the study, not having any disease, and no allergies to honey-derived foods were determined. The supplementation group was given liquid royal jelly (n = 10/1000 mg / day), which was kept refrigerated in glass vials at the same time every morning for three weeks. The control group was not given any supplementation.

Blood Test Procedure
5 ml of venous blood samples were collected from the right arm in yellow cap tubes between 09:00-10:30 in the morning in Gaziantep University biochemistry laboratory before and after 1 day of royal jelly supplementation. Blood samples were centrifuged in Nüve-NF800 apparatus at 4000 rpm for a total of 7 minutes and their sera were separated. The Spectrophotometric method was used to determine serum levels in both groups; Creatinine and Protein levels were analyzed.

Statistical Analysis
SPSS 22.0 package program was used for statistical analysis of the measured data of the supplementation and control groups. Data were presented as mean and standard deviation. Statistical results were evaluated at p<0.05 significance level. For the analysis of the measured data of the supplementation and Control groups 2x2 mixed factor ANOVA and LSD tests were performed.

RESULTS
The data of the researches are presented in the table as mean and standard deviation.

CONCLUSION
Creatinine and Protein levels of royal jelly supplement given to cigarette addicts were investigated. There were changes in Creatinine and Protein levels in the supplementation and control groups, but these changes were not statistically significant. The content of royal jelly varies according to natural feeding of bees, season and age of larvae and production method. The water-soluble pH of 3-5 royal jelly contains proteins, lipids and carbohydrates. Vigor, vitality, because of the thought that contributes to cell renewal; royal jelly, which is widely consumed by humans, has been reported to contain very low amounts of biologically active substances such as ptrein, neopterin, biopterin, xantopterin and hormones (Rembold and Dietz, 1965). While plasma proteins have critical roles in defending the body and regulating body water balance, proteins are primarily involved in the binding and transport of a large number of molecules, such as nutrients, metabolites, hormones and drugs (Michael, Janet, Edwards, 2000;Shauna and Susan, 1993). In the studies, although plasma total protein concentration changes in very small amounts with aging, especially albumin levels decrease with aging, whereas alpha-1 antitrypsin, alpha-1 acid glycoprotein and gamma globulin levels increase and haptoglobin levels do not change (Rochman, 1998;Tietz, Shaey, Welcstein, 1994). Creatinine is a chemical waste product produced from muscle metabolism. Serum creatinine and an associated increase in blood urea nitrogen often reflect a decrease in Glomerular filtration rate. Creatinine is a creatine phosphate metabolite that acts as an energy source in muscle. Although this molecule varies depending on muscle mass, it is produced at a fairly constant rate in the body (Özkan and Ulusoy, 2016). In our study, it was observed that royal jelly supplementation did not make any difference in creatinine and protein levels. Royal jelly is mostly used in the treatment of diseases such as bronchial asthma, arteriosclerosis, stomach and intestinal diseases, rheumatism. In addition, it has been stated that it has high blood pressure prevention and kidney and urinary tract disorders. Royal jelly as well as mental and physical fatigue, as well as the aging of the skin and against the deterioration of the skin is used effectively against stress (Anonymous, 1992). It is stated that royal jelly lowers blood cholesterol, total lipid, phospholipid, triglyceride, blipoprotein levels, has a blood pressure lowering and vasodilatory activity, and has insulin-like peptides, hypoglycemic (lowering blood sugar) and immunological effects. In addition to these therapeutic role in skin and hair diseases, sexual functions, as well as regulatory effects, cell repair and rejuvenating effects have been emphasized (Meydanoğlu, 1985;Yatsunami and Echigo, 1985). As a result, it can be said that royal jelly supplementation does not affect creatinine and protein levels.